Splenocytes were cultured and stimulated with Anti-Mouse CD3 and Anti-Mouse CD28 for 3 days or harvested fresh on day 3 and then surface-stained with 0.25 ug of Anti-Mouse CD213a1 (IL-13Ra1) PE. The plot is an overlay of the stimulated cells (purple) and freshly isolated splenocytes (blue). Isotype control staining of the stimulated cells is indicated by the dotted line. Cells in the CD4+ lymphocyte gate were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The 13MOKA monoclonal antibody reacts with mouse CD213 alpha 1 (interleukin-13 receptor alpha 1, IL-13Ra1). CD213a1 is the low-affinity IL-13 receptor which mediates normal IL-13 inflammatory signaling. It has been shown that CD213a1 binds to IL-13 which then recruits CD124 (IL-4Ralpha) to stabilize the low-affinity interaction and form the active signaling complex. The CD213a1/CD124 complex can also serve as a receptor for IL-4, although the role of CD213a1 in this complex is poorly understood. CD213a1 is expressed on activated B and T cells and other cells.
Applications Reported: This 13MOKA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 13MOKA antibody has been tested on mouse splenocytes stimulated for 3 days with Anti-CD3 and Anti-CD28 Functional Grade antibodies. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||A functional IL-13 receptor is expressed on polarized murine CD4+ Th17 cells and IL-13 signaling attenuates Th17 cytokine production.||Newcomb DC,Zhou W,Moore ML,Goleniewska K,Hershey GK,Kolls JK,Peebles RS||Journal of immunology (Baltimore, Md. : 1950) (182:5317)||2009|
|Not Applicable||Not Cited||Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.||Munitz A,Brandt EB,Mingler M,Finkelman FD,Rothenberg ME||Proceedings of the National Academy of Sciences of the United States of America (105:7240)||2008|
|Not Applicable||Not Cited||Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system.||LaPorte SL,Juo ZS,Vaclavikova J,Colf LA,Qi X,Heller NM,Keegan AD,Garcia KC||Cell (132:259)||2008|
|Not Applicable||Not Cited||Regulation of IL-13 receptor alpha 1 expression and signaling on human tonsillar B-lymphocyte subsets.||Hajoui O,Zheng H,Guay J,Letuve S,Fawaz LM,Mazer BD||The Journal of allergy and clinical immunology (120:1425)||2007|