|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Protein encoding the external domain of human CD22|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:30|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-11421 targets CD22 in IHC (P) and Western blot applications and shows reactivity with Human samples. This antibody is not recommended for Raji cells in Western blot applications.
The MA5-11421 immunogen is recombinant Protein encoding the external domain of human CD22.
CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types.In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+.It is not expressed by plasma cells. CD22 is found highly expressed in follicular mantle and marginal zone B-cells, while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Rituximab-induced changes in hematolymphoid tissues found at autopsy.
MA5-11421 was used in immunohistochemistry to investigate the effect of rituximab on inducing depletion of B lymphocyte populations
|Cioc AM,Vanderwerf SM,Peterson BA,Robu VG,Forster CL,Pambuccian SE||American journal of clinical pathology (130:604)||2008|