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Invitrogen
CD229 (Ly-9) Monoclonal Antibody (HLy9.25), APC, eBioscience™
-Flow-20170216130641.jpg?time=20240507 "CD229 (Ly-9) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
CD229 (Ly-9) Antibody (17-2299-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD3 PerCP-Cy5.5 (Product # 45-0037-41) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD229 (Ly-9) APC (right). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20240507)
-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD229 (Ly-9) Antibody (17-2299-42) in Flow")
Product Details
17-2299-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HLy9.25
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1311158
Product Specific Information
Description: This HLy9.25 monoclonal antibody reacts with human CD229 (also known as Ly9 or SLAMF3), an approximately 120-kDa member of the Signaling Lymphocyte Activation Molecule (SLAM) immunoglobulin superfamily. This type I transmembrane glycoprotein is expressed on a variety of immune cell types, including thymocytes, T and B lymphocytes, and natural killer cells. Expression of CD229 is also detected on T and B cell malignancies, including Jurkat and Burkitt lymphoma cells, respectively. CD229 has been shown to interact with SH2-binding phosphatases and the adaptor proteins SLAM-associated protein (SAP) and EAT-2. Moreover, CD229 localizes to the immunological synapse following T cell activation.
Applications Reported: This HLy9.25 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HLy9.25 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD229; Cell surface molecule Ly-9; Lymphocyte antigen 9; RP11-312J18.1; Signaling lymphocytic activation molecule 3; SLAM family member 3; SLAMF3; T-lymphocyte surface antigen Ly-9
Gene Aliases: CD229; CDABP0070; hly9; LY9; mLY9; SLAMF3
UniProt ID: (Human) Q6P2J4
Entrez Gene ID: (Human) 4063
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