|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding human CD23 48-248aa|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inadequate T follicular cell help impairs B cell immunity during HIV infection.
MA5-16335 was used in immunohistochemistry to study the expansion of follicular helper T-cells that fail to provide adequate help to B-cells in individuals with HIV infection
|Cubas RA,Mudd JC,Savoye AL,Perreau M,van Grevenynghe J,Metcalf T,Connick E,Meditz A,Freeman GJ,Abesada-Terk G,Jacobson JM,Brooks AD,Crotty S,Estes JD,Pantaleo G,Lederman MM,Haddad EK||Nature medicine (19:494)||2013|
Pediatric lymphomas in Brazil.
MA5-16335 was used in immunohistochemistry to study the characteristics of pediatric lymphomas in the Brazilian population
|Gualco G,Klumb CE,Barber GN,Weiss LM,Bacchi CE||Clinics (Sao Paulo, Brazil) (65:1267)||2011|
Cyclin D1 expression in B-cell lymphomas.
MA5-16335 was used in immunohistochemistry to study the expression of cyclin D1 in B-cell lymphomas
|Gladkikh A,Potashnikova D,Korneva E,Khudoleeva O,Vorobjev I||Experimental hematology (38:1047)||2010|