Invitrogen

CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), APC, eBioscience™

Product Image Gallery

CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control APC (Product # 17-4732-81) (blue histogram) or Anti-Human CD235a (Glycophorin A) APC (purple histogram). Non-leukocytes were used for analysis.

Product Details

17-9987-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.015 µg)/test	View 24 publications 24 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2b, kappa
Recommended Isotype Control	Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HIR2 (GA-R2)
Conjugate	APC Alexa Fluor 700 APC-eFluor 780 Biotin eFluor 450 FITC PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2043823

Product Specific Information

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

The immunoregulatory function of peripheral blood CD71⁺ erythroid cells in systemic-onset juvenile idiopathic arthritis.

Scientific reports

Kanemasa H,Ishimura M,Eguchi K,Tanaka T,Nanishi E,Shiraishi A,Goto M,Motomura Y,Ohga S

17-9987-42 was used in Flow Cytometry to conclude that circulating CECs may control excessive inflammation via the immunoregulatory pathways, partly involving arginase-2.

Tue Jul 13 00:00:00 EDT 2021

Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

Frontiers in cell and developmental biology

García-Montolio M,Ballaré C,Blanco E,Gutiérrez A,Aranda S,Gómez A,Kok CH,Yeung DT,Hughes TP,Vizán P,Di Croce L

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Extracellular vesicles are associated with C-reactive protein in sepsis.

Scientific reports

Fendl B,Weiss R,Eichhorn T,Linsberger I,Afonyushkin T,Puhm F,Binder CJ,Fischer MB,Weber V

17-9987 was used in Flow cytometry/Cell sorting to provide evidence that CRP+ EVs exhibit pro-inflammatory characteristics and can contribute to the spreading of inflammation throughout the circulation on top of their pro-coagulant activity.

Fri Mar 26 00:00:00 EDT 2021

Iron control of erythroid microtubule cytoskeleton as a potential target in treatment of iron-restricted anemia.

Nature communications

Goldfarb AN,Freeman KC,Sahu RK,Elagib KE,Holy M,Arneja A,Polanowska-Grabowska R,Gru AA,White Z,Khalil S,Kerins MJ,Ooi A,Leitinger N,Luckey CJ,Delehanty LL

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Fri Mar 12 00:00:00 EST 2021

Common variants in signaling transcription-factor-binding sites drive phenotypic variability in red blood cell traits.

Nature genetics

Choudhuri A,Trompouki E,Abraham BJ,Colli LM,Kock KH,Mallard W,Yang ML,Vinjamur DS,Ghamari A,Sporrij A,Hoi K,Hummel B,Boatman S,Chan V,Tseng S,Nandakumar SK,Yang S,Lichtig A,Superdock M,Grimes SN,Bowman TV,Zhou Y,Takahashi S,Joehanes R,Cantor AB,Bauer DE,Ganesh SK,Rinn J,Albert PS,Bulyk ML,Chanock SJ,Young RA,Zon LI

17-9987 was used in Flow cytometry/Cell sorting to propose that the majority of the RBC-trait-associated variants that reside on transcription-factor-binding sequences fall in STF target sequences, suggesting that the phenotypic variation of RBC traits could stem from altered responsiveness to extracellular stimuli.

Tue Dec 01 00:00:00 EST 2020

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Genome-Wide Transcriptional Regulation of the Long Non-coding RNA Steroid Receptor RNA Activator in Human Erythroblasts.

Frontiers in genetics

Sawaengdee W,Cui K,Zhao K,Hongeng S,Fucharoen S,Wongtrakoongate P

17-9987 was used in Flow cytometry/Cell sorting to establish that the lncRNA SRA occupies chromatin, and promotes transcription of erythroid genes, therefore facilitating human erythroid transcriptional program.

Mon Sep 28 00:00:00 EDT 2020

Proteosomal degradation of NSD2 by BRCA1 promotes leukemia cell differentiation.

Communications biology

Park JW,Kang JY,Hahm JY,Kim HJ,Seo SB

17-9987 was used in Flow cytometry/Cell sorting to study the regulation of NSD2 stability by BRCA1-mediated ubiquitination as a potential therapeutic target process in multiple myeloma.

Fri Aug 21 00:00:00 EDT 2020

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

17-9987-42 was used in Flow Cytometry to reveal stage-specific transcriptional states and chromatin accessibility during various stages of erythropoiesis.

Tue Jun 11 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

EDAG promotes the expansion and survival of human CD34+ cells.

PloS one

Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

17-9987 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.

Tue Feb 06 00:00:00 EST 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

17-9987 was used in Flow cytometry/Cell sorting to demonstrate how variation in a single cytokine can lead to biased downstream signaling and can thereby cause human disease.

Thu Mar 09 00:00:00 EST 2017

The Impact of Lipoprotein-Associated Oxidative Stress on Cell-Specific Microvesicle Release in Patients with Familial Hypercholesterolemia.

Oxidative medicine and cellular longevity

Nielsen MH,Irvine H,Vedel S,Raungaard B,Beck-Nielsen H,Handberg A

17-9987 was used in Flow cytometry/Cell sorting to measure cell-specific microvesicles in familial hypercholesterolemia patients with and without Achilles tendon xanthomas, and their association with atherogenic lipid profile.

Tue Dec 13 00:00:00 EST 2016

Platelets confound the measurement of extracellular miRNA in archived plasma.

Scientific reports

Mitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2016

LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.

Nature communications

Lu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J

17-9987 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which cytokines in the blood affect HSC homeostasis, showing that LECT2 drives HSC expansion and mobilisation.

Tue Sep 06 00:00:00 EDT 2016

Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2016

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.

Stem cells (Dayton, Ohio)

Zheng WW,Dong XM,Yin RH,Xu FF,Ning HM,Zhang MJ,Xu CW,Yang Y,Ding YL,Wang ZD,Zhao WB,Tang LJ,Chen H,Wang XH,Zhan YQ,Yu M,Ge CH,Li CY,Yang XM

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Altered translation of GATA1 in Diamond-Blackfan anemia.

Nature medicine

Ludwig LS,Gazda HT,Eng JC,Eichhorn SW,Thiru P,Ghazvinian R,George TI,Gotlib JR,Beggs AH,Sieff CA,Lodish HF,Lander ES,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

A flow cytometric method for characterization of circulating cell-derived microparticles in plasma.

Journal of extracellular vesicles

Nielsen MH,Beck-Nielsen H,Andersen MN,Handberg A

17-9987 was used in Flow cytometry/Cell sorting to establish a method to analyze microparticle subpopulations above the threshold of detection of a new generation BD FACSAriaTM III digital flow cytometer.

Mon Feb 10 00:00:00 EST 2014

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 17-9987-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha

Gene Aliases: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2

UniProt ID: (Human) P02724

Entrez Gene ID: (Human) 2993

Function(s)

viral entry into host cell

Process(es)

virus receptor activity identical protein binding