Invitrogen

CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PerCP-eFluor™ 710, eBioscience™

Product Image Gallery

CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD235a (Glycophorin A) PerCP-eFluor® 710 (purple histogram). Non-leukocytes were used for analysis.

Product Details

46-9987-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.002 µg)/test	View 12 publications 12 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG2b, kappa
Recommended Isotype Control	Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HIR2 (GA-R2)
Conjugate	PerCP-eFluor™ 710 Alexa Fluor 700 APC APC-eFluor 780 Biotin eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 685 NovaFluor Red 700 NovaFluor Yellow 610 NovaFluor Yellow 660 PE PE-Cyanine7 PE-eFluor 610 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1944359

Product Specific Information

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analsyis of human whole blood. This can be used at 5 µL (0.002 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

Frontiers in cell and developmental biology

García-Montolio M,Ballaré C,Blanco E,Gutiérrez A,Aranda S,Gómez A,Kok CH,Yeung DT,Hughes TP,Vizán P,Di Croce L