Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Flow Cytometry (Flow) |
5 µL (0.002 µg)/test | View 12 publications 12 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Not Applicable |
Host/Isotype |
Mouse / IgG2b, kappa |
Recommended Isotype Control |
Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
HIR2 (GA-R2) |
Conjugate |
PerCP-eFluor™ 710
|
Excitation/Emission Profile |
View spectra |
Form |
Liquid |
Concentration |
5 µL/Test |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_1944359 |
Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.
Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analsyis of human whole blood. This can be used at 5 µL (0.002 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha
Gene Aliases: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2
UniProt ID: (Human) P02724
Entrez Gene ID: (Human) 2993
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