Invitrogen

CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PerCP-eFluor™ 710, eBioscience™

Product Image Gallery

CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD235a (Glycophorin A) PerCP-eFluor® 710 (purple histogram). Non-leukocytes were used for analysis.

Product Details

46-9987-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.002 µg)/test	View 12 publications 12 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG2b, kappa
Recommended Isotype Control	Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HIR2 (GA-R2)
Conjugate	PerCP-eFluor™ 710 Alexa Fluor 700 APC APC-eFluor 780 Biotin eFluor 450 FITC PE PE-Cyanine7 PE-eFluor 610 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1944359

Product Specific Information

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analsyis of human whole blood. This can be used at 5 µL (0.002 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

Frontiers in cell and developmental biology

García-Montolio M,Ballaré C,Blanco E,Gutiérrez A,Aranda S,Gómez A,Kok CH,Yeung DT,Hughes TP,Vizán P,Di Croce L