Invitrogen

CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eFluor™ 450, eBioscience™

Product Image Gallery

CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (blue histogram) or Anti-Human CD235a (Glycophorin A) eFluor® 450 (purple histogram). All non-leukocytes were used for analysis.

CD235a (Glycophorin A) Antibody in Immunocytochemistry (ICC/IF) — Figure 3 (A) Morphological comparison of sarcomas, reprogrammed sarcomas, and reprogrammed sarcomas at days 7, 14, 21 and 28 of an erythroid differentiation protocol. Scale bars=10uM. (B) Individual cells on the verge of enucleation from day 28 erythroid differentiation cultures. Scale bars=5uM. The original magnification for all panels in A were x200; and x1000 for B. (C) Percentage of cells from HOS, SW872, SKNEP, Rep-HOS, Rep-SW872, and Rep-SKNEP cultures undergoing erythroid differentiation expressing CD71, CD235a (GPA), CD36, and CD34. Error bars=standard deviation. (D) CD71 and CD235(GPA) immunofluorescence in reprogrammed SW872 cells after 14 and 28 days of erythroid differentiation. green circle=CD235a+/DAPI+; blue circle=CD235-/DAPI+; yellow circle=CD235a+/DAPI-. Scale bars=10um.

Product Details

48-9987-42

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	5 µL (0.03 µg)/test	View 15 publications 15 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2b, kappa
Recommended Isotype Control	Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	HIR2 (GA-R2)
Conjugate	eFluor™ 450 Alexa Fluor 700 APC APC-eFluor 780 Biotin FITC NovaFluor Blue 510 NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 685 NovaFluor Red 700 NovaFluor Yellow 610 NovaFluor Yellow 660 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574141

Product Specific Information

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of diluted normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

Frontiers in cell and developmental biology

García-Montolio M,Ballaré C,Blanco E,Gutiérrez A,Aranda S,Gómez A,Kok CH,Yeung DT,Hughes TP,Vizán P,Di Croce L

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Iron control of erythroid microtubule cytoskeleton as a potential target in treatment of iron-restricted anemia.

Nature communications

Goldfarb AN,Freeman KC,Sahu RK,Elagib KE,Holy M,Arneja A,Polanowska-Grabowska R,Gru AA,White Z,Khalil S,Kerins MJ,Ooi A,Leitinger N,Luckey CJ,Delehanty LL

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Fri Mar 12 00:00:00 EST 2021

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

48-9987 was used in Flow cytometry/Cell sorting to identify a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74- and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF-β3) and decreased levels of TGF-β1.

Sun Nov 01 00:00:00 EDT 2020

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

Sun Nov 01 00:00:00 EDT 2020

Pro-Fibrotic CD26-Positive Fibroblasts Are Present in Greater Abundance in Breast Capsule Tissue of Irradiated Breasts.

Aesthetic surgery journal

Borrelli MR,Irizzary D,Patel RA,Nguyen D,Momeni A,Longaker MT,Wan DC

48-9987-42 was used in Flow Cytometry to investigate the role of CD26-positive fibroblasts in the formation of breast implant capsules following radiation therapy.

Mon Mar 23 00:00:00 EDT 2020

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

Genetic programming of macrophages generates an in vitro model for the human erythroid island niche.

Nature communications

Lopez-Yrigoyen M,Yang CT,Fidanza A,Cassetta L,Taylor AH,McCahill A,Sellink E,von Lindern M,van den Akker E,Mountford JC,Pollard JW,Forrester LM

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Wed Feb 20 00:00:00 EST 2019

Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease.

Nature communications

Moss J,Magenheim J,Neiman D,Zemmour H,Loyfer N,Korach A,Samet Y,Maoz M,Druid H,Arner P,Fu KY,Kiss E,Spalding KL,Landesberg G,Zick A,Grinshpun A,Shapiro AMJ,Grompe M,Wittenberg AD,Glaser B,Shemer R,Kaplan T,Dor Y

48-9987 was used in Flow cytometry/Cell sorting to develop a protocol for unbiased determination of the tissue origins of circulating cell-free DNA, using a reference methylation atlas of 25 human tissues and cell types.

Thu Nov 29 00:00:00 EST 2018

Platelets confound the measurement of extracellular miRNA in archived plasma.

Scientific reports

Mitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2016

Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.

Stem cells (Dayton, Ohio)

Zheng WW,Dong XM,Yin RH,Xu FF,Ning HM,Zhang MJ,Xu CW,Yang Y,Ding YL,Wang ZD,Zhao WB,Tang LJ,Chen H,Wang XH,Zhan YQ,Yu M,Ge CH,Li CY,Yang XM

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Altered translation of GATA1 in Diamond-Blackfan anemia.

Nature medicine

Ludwig LS,Gazda HT,Eng JC,Eichhorn SW,Thiru P,Ghazvinian R,George TI,Gotlib JR,Beggs AH,Sieff CA,Lodish HF,Lander ES,Sankaran VG

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Immunofluorescence

Thu May 02 00:00:00 EDT 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD235a (Glycophorin A) monoclonal antibody (Product # 48-9987-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha

Gene Aliases: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2

UniProt ID: (Human) P02724

Entrez Gene ID: (Human) 2993

Function(s)

viral entry into host cell

Process(es)

virus receptor activity identical protein binding