Staining of C57BL/6 splenocytes with an anti-mouse CD3-eFluor® 450 antibody and 0.06 μg of a rat IgG1,K-PE-Cy®7 isotype control (left), or 0.06 μg of rat anti-mouse CD24-PE-Cy®7 monoclonal antibody (Cat. no. A14776) (right). Cells in the lymphocyte gate were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||mouse CD24 antigen.|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. It is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics.
A14776 was used in flow cytometry to demonstrate that proliferating neural stem cells lengthen their G1 phase during aging.
|Daynac M,Morizur L,Kortulewski T,Gauthier LR,Ruat M,Mouthon MA,Boussin FD||Journal of visualized experiments : JoVE (null:null)||2015|