Immunofluorescent analysis of CD24 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11828) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 13 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Flow Cytometry (Flow)||See 4 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
MA5-11828 targets CD24 in FACS, IHC (F), ICC/IF and WB applications and shows reactivity with Human and Mouse samples. This antibody detects a few non-specific bands in BAF-3 cell lysates.
The MA5-11828 immunogen is cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line.
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. It is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Differential expression of ligands for NKG2D and DNAM-1 receptors by epithelial ovarian cancer-derived exosomes and its influence on NK cell cytotoxicity.
MA5-11828 was used in immunocytochemistry and western blot to characterize the influence on NK cell cytotoxicity by differential expression of ligands for DNAM-1 and NKG2D receptors by epithelial ovarian cancer-derived exosomes
|Labani-Motlagh A,Israelsson P,Ottander U,Lundin E,Nagaev I,Nagaeva O,Dehlin E,Baranov V,Mincheva-Nilsson L||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (37:5455)||2016|
|Not Applicable||Not Cited||
CD24 Overexpression Is Associated with Poor Prognosis in Luminal A and Triple-Negative Breast Cancer.
MA5-11828 was used in western blot to elucidate a poor prognosis in triple-negative breast cancer and luminal A by CD24 overexpression
|Kwon MJ,Han J,Seo JH,Song K,Jeong HM,Choi JS,Kim YJ,Lee SH,Choi YL,Shin YK||PloS one (10:null)||2015|
CD24 expression as a marker for predicting clinical outcome in human gliomas.
MA5-11828 was used in western blot to study the prognostic value of CD24 expression in human gliomas
|Deng J,Gao G,Wang L,Wang T,Yu J,Zhao Z||Journal of biomedicine and biotechnology (2012:null)||2012|
CD24 expression is a novel prognostic factor in esophageal squamous cell carcinoma.
MA5-11828 was used in western blot to study the prognostic value of CD24 overexpression in esophageal squamous cell carcinoma
|Sano A,Kato H,Sakurai S,Sakai M,Tanaka N,Inose T,Saito K,Sohda M,Nakajima M,Nakajima T,Kuwano H||Annals of surgical oncology (16:506)||2009|
The role of extracellular factors in human metastatic chordoma cell growth in vitro.
MA5-11828 was used in western blot to study the role of the extracellular microenvironment in human metastatic chordoma cell growth in vitro
|Ostroumov E,Hunter CJ||Spine (32:2957)||2007|
The clinicopathological and prognostic significance of CD24, CD44, CD133, ALDH1 expressions in invasive ductal carcinoma of the breast: CD44/CD24 expression in breast cancer.
MA5-11828 was used in immunohistochemistry to evaluate the expression of CD24, CD44, CD133 and ALDH1 in invasive ductal carcinoma of the breast
|Kapucuo¿lu N,Bozkurt KK,Ba¿p¿nar ¿,Koçer M,Ero¿lu HE,Akdeniz R,Akçil M||Pathology, research and practice (211:740)||2015|
Fibromatosis-like metaplastic carcinoma of the breast has a claudin-low immunohistochemical phenotype.
MA5-11828 was used in immunohistochemistry to study the claudin expression in fibromatosis-like metaplastic carcinoma of the breast
|Rito M,Schmitt F,Pinto AE,André S||Virchows Archiv : an international journal of pathology (465:185)||2014|
CD24 and CD44 in salivary gland pleomorphic adenoma and in human salivary gland morphogenesis: differential markers of glandular structure or stem cell indicators?
MA5-11828 was used in immunohistochemistry to study whether CD24 and CD44 expression in salivary glands can be used as markers of cancer stem cells
|Ianez RC,Coutinho-Camillo CM,Buim ME,Pinto CA,Soares FA,Lourenço SV||Histopathology (62:1075)||2013|
Acetaminophen-induced differentiation of human breast cancer stem cells and inhibition of tumor xenograft growth in mice.
MA5-11828 was used in immunohistochemistry to screen compounds able to induce differentiation of cancer stem cells
|Takehara M,Hoshino T,Namba T,Yamakawa N,Mizushima T||Biochemical pharmacology (81:1124)||2011|
Different prognostic significance of CD24 and CD44 expression in breast cancer according to hormone receptor status.
MA5-11828 was used in immunohistochemistry to study the clinical significance of CD24 and CD44 expression in breast cancer.
|Kim HJ,Kim MJ,Ahn SH,Son BH,Kim SB,Ahn JH,Noh WC,Gong G||Breast (Edinburgh, Scotland) (20:78)||2011|
CD24 is a novel predictor for poor prognosis of hepatocellular carcinoma after surgery.
MA5-11828 was used in immunohistochemistry to study the role of CD24 in tumor invasion and its prognostic significance in hepatocellular carcinoma
|Yang XR,Xu Y,Yu B,Zhou J,Li JC,Qiu SJ,Shi YH,Wang XY,Dai Z,Shi GM,Wu B,Wu LM,Yang GH,Zhang BH,Qin WX,Fan J||Clinical cancer research : an official journal of the American Association for Cancer Research (15:5518)||2009|
Association of breast cancer stem cells identified by aldehyde dehydrogenase 1 expression with resistance to sequential Paclitaxel and epirubicin-based chemotherapy for breast cancers.
MA5-11828 was used in immunohistochemistry to study whether cancer stem cells are clinically significant for resistance to chemotherapy in human breast cancers
|Tanei T,Morimoto K,Shimazu K,Kim SJ,Tanji Y,Taguchi T,Tamaki Y,Noguchi S||Clinical cancer research : an official journal of the American Association for Cancer Research (15:4234)||2009|
Laser capture microdissection-microarray analysis of focal segmental glomerulosclerosis glomeruli.
MA5-11828 was used in immunohistochemistry to analyse the focal segmental glomerulosclerosis glomeruli by laser capture microdissection-microarray method
|Bennett MR,Czech KA,Arend LJ,Witte DP,Devarajan P,Potter SS||Nephron. Experimental nephrology (107:e30)||2007|
CD24 expression is a prognostic factor in intrahepatic cholangiocarcinoma.
MA5-11828 was used in immunohistochemistry to study CD24 expression as a prognostic factor in intrahepatic cholangiocarcinoma
|Su MC,Hsu C,Kao HL,Jeng YM||Cancer letters (235:34)||2006|
Cytoplasmic CD24 expression in colorectal cancer independently correlates with shortened patient survival.
MA5-11828 was used in immunohistochemistry to study the prognostic value of cytoplasmic CD24 expression in colorectal cancer
|Weichert W,Denkert C,Burkhardt M,Gansukh T,Bellach J,Altevogt P,Dietel M,Kristiansen G||Clinical cancer research : an official journal of the American Association for Cancer Research (11:6574)||2005|
Molecular and prognostic distinction between serous ovarian carcinomas of varying grade and malignant potential.
MA5-11828 was used in immunohistochemistry to distinguish low malignant potential serous ovarian carcinomas from invasive counterparts
|Meinhold-Heerlein I,Bauerschlag D,Hilpert F,Dimitrov P,Sapinoso LM,Orlowska-Volk M,Bauknecht T,Park TW,Jonat W,Jacobsen A,Sehouli J,Luttges J,Krajewski M,Krajewski S,Reed JC,Arnold N,Hampton GM||Oncogene (24:1053)||2005|
Expression profiling of microdissected matched prostate cancer samples reveals CD166/MEMD and CD24 as new prognostic markers for patient survival.
MA5-11828 was used in immunohistochemistry to profile the expression patterns of microdissected prostate cancer samples to identify new prognostic markers
|Kristiansen G,Pilarsky C,Wissmann C,Kaiser S,Bruemmendorf T,Roepcke S,Dahl E,Hinzmann B,Specht T,Pervan J,Stephan C,Loening S,Dietel M,Rosenthal A||The Journal of pathology (205:359)||2005|
CD24 expression is a significant predictor of PSA relapse and poor prognosis in low grade or organ confined prostate cancer.
MA5-11828 was used in immunohistochemistry to study the prognostic value of CD24 expression in low grade or organ confined prostate cancer
|Kristiansen G,Pilarsky C,Pervan J,Stürzebecher B,Stephan C,Jung K,Loening S,Rosenthal A,Dietel M||The Prostate (58:183)||2004|
Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer.
MA5-11828 was used in flow cytometry to assess the effects of palladium(II) and niclosamide on the Wnt signaling pathway associated with breast cancer stem cells
|Karakas D,Cevatemre B,Aztopal N,Ari F,Yilmaz VT,Ulukaya E||Bioorganic and medicinal chemistry (23:5580)||2015|
Multiparameter flow cytometry and bioanalytics for B cell profiling in systemic lupus erythematosus.
MA5-11828 was used in flow cytometry to generate specialized B cell reagent panels to characterize B cell disorders.
|Kaminski DA,Wei C,Rosenberg AF,Lee FE,Sanz I||Methods in molecular biology (Clifton, N.J.) (900:109)||2012|
Diversity, cellular origin and autoreactivity of antibody-secreting cell population expansions in acute systemic lupus erythematosus.
MA5-11828 was used in flow cytometry to examine the antibody repertoire of patients with acute systemic lupus erythematosus.
|Tipton CM,Fucile CF,Darce J,Chida A,Ichikawa T,Gregoretti I,Schieferl S,Hom J,Jenks S,Feldman RJ,Mehr R,Wei C,Lee FE,Cheung WC,Rosenberg AF,Sanz I||Nature immunology (16:755)||2015|
|Not Applicable||Not Cited||
Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers.
MA5-11828 was used in flow cytometry to determine the phenotype and function of stem/progenitor cells in normal mammary epithelial cell populations and their malignant counterparts
|Ghebeh H,Sleiman GM,Manogaran PS,Al-Mazrou A,Barhoush E,Al-Mohanna FH,Tulbah A,Al-Faqeeh K,Adra CN||BMC cancer (13:null)||2013|
|Not Applicable||Not Cited||
A new population of cells lacking expression of CD27 represents a notable component of the B cell memory compartment in systemic lupus erythematosus.
MA5-11828 was used in flow cytometry to discuss the frequency and contribution of CD27- memory B cells to systemic lupus erythematosus
|Wei C,Anolik J,Cappione A,Zheng B,Pugh-Bernard A,Brooks J,Lee EH,Milner EC,Sanz I||Journal of immunology (Baltimore, Md. : 1950) (178:6624)||2007|
|Not Applicable||Not Cited||
Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.
MA5-11828 was used in flow cytometry to evaluate the use of flow cytometry to identify the Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma
|Fromm JR,Kussick SJ,Wood BL||American journal of clinical pathology (126:764)||2006|
Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors.
MA5-11828 was used in immunohistochemistry - paraffin section to characterize Brazilian breast cancer cell lines
|Corrêa NC,Kuasne H,Faria JA,Seixas CC,Santos IG,Abreu FB,Nonogaki S,Rocha RM,Aparecida Borges Silva G,Gobbi H,Rogatto SR,Goes AM,Gomes DA||Oncology reports (29:1299)||2013|