Invitrogen

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), APC-eFluor™ 780, eBioscience™

Product Image Gallery

CD24 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD24 APC-eFluor® 780 (purple histogram). Cells in the granulocyte gate were used for analysis.

Product Details

47-0247-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 21 publications 21 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBioSN3 (SN3 A5-2H10)
Conjugate	APC-eFluor™ 780 Alexa Fluor 700 APC Biotin eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 585 NovaFluor Blue 690 NovaFluor Red 725 NovaFluor Yellow 570 NovaFluor Yellow 660 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10735091

Product Specific Information

Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Elevated transcription and glycosylation of B3GNT5 promotes breast cancer aggressiveness.

Journal of experimental & clinical cancer research : CR

Miao Z,Cao Q,Liao R,Chen X,Li X,Bai L,Ma C,Deng X,Dai Z,Li J,Dong C

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Sat May 07 00:00:00 EDT 2022

Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.

Oxidative medicine and cellular longevity

Chen R,Gao S,Guan H,Zhang X,Gao Y,Su Y,Song Y,Jiang Y,Li N

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Thu Mar 24 00:00:00 EDT 2022

A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yu S,Lu Y,Su A,Chen J,Li J,Zhou B,Liu X,Xia Q,Li Y,Li J,Huang M,Ye Y,Zhao Q,Jiang S,Yan X,Wang X,Di C,Pan J,Su S

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α.

Cancer management and research

Gu J,Xu T,Huang QH,Zhang CM,Chen HY

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling.

International journal of molecular sciences

Sabol RA,Villela VA,Denys A,Freeman BT,Hartono AB,Wise RM,Harrison MAA,Sandler MB,Hossain F,Miele L,Bunnell BA

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

BRCA1 regulates the cancer stem cell fate of breast cancer cells in the context of hypoxia and histone deacetylase inhibitors.

Scientific reports

Kim H,Lin Q,Yun Z

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2019

Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.

Theranostics

Cao Y,Lin SH,Wang Y,Chin YE,Kang L,Mi J

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Mon Apr 30 00:00:00 EDT 2018

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Tue Mar 06 00:00:00 EST 2018

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Molecular cancer

Shah M,Cardenas R,Wang B,Persson J,Mongan NP,Grabowska A,Allegrucci C

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Thu Feb 16 00:00:00 EST 2017

Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.

Oncotarget

Greenwood E,Maisel S,Ebertz D,Russ A,Pandey R,Schroeder J

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Tue Sep 20 00:00:00 EDT 2016

The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.

BMC biology

Toneff MJ,Sreekumar A,Tinnirello A,Hollander PD,Habib S,Li S,Ellis MJ,Xin L,Mani SA,Rosen JM

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Fri Jun 17 00:00:00 EDT 2016

Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1.

Molecular medicine reports

Sun Z,Zhang C,Zou X,Jiang G,Xu Z,Li W,Xie H

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1.

Scientific reports

Lin Y,Liu AY,Fan C,Zheng H,Li Y,Zhang C,Wu S,Yu D,Huang Z,Liu F,Luo Q,Yang CJ,Ouyang G

47-0247 was used in Flow cytometry/Cell sorting to investigate how miR-33b acts as an onco-suppressive microRNA in breast cancer progression by inhibiting the stemness and metastasis of breast cancer cells.

Tue Apr 28 00:00:00 EDT 2015

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Oncogene

Cyr AR,Kulak MV,Park JM,Bogachek MV,Spanheimer PM,Woodfield GW,White-Baer LS,O'Malley YQ,Sugg SL,Olivier AK,Zhang W,Domann FE,Weigel RJ

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Thu Jan 22 00:00:00 EST 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Tue Jul 15 00:00:00 EDT 2014

Twist2 promotes self-renewal of liver cancer stem-like cells by regulating CD24.

Carcinogenesis

Liu AY,Cai Y,Mao Y,Lin Y,Zheng H,Wu T,Huang Y,Fang X,Lin S,Feng Q,Huang Z,Yang T,Luo Q,Ouyang G

47-0247 was used in Flow cytometry/Cell sorting to demonstrate that Twist2 promotes self-renewal in liver cancer stem-like cells by regulating CD24.

Sat Mar 01 00:00:00 EST 2014

Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay.

Gastroenterology

Wang F,Scoville D,He XC,Mahe MM,Box A,Perry JM,Smith NR,Lei NY,Davies PS,Fuller MK,Haug JS,McClain M,Gracz AD,Ding S,Stelzner M,Dunn JC,Magness ST,Wong MH,Martin MG,Helmrath M,Li L

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.

Breast cancer research : BCR

Iwanaga R,Wang CA,Micalizzi DS,Harrell JC,Jedlicka P,Sartorius CA,Kabos P,Farabaugh SM,Bradford AP,Ford HL

Published figure using CD24 monoclonal antibody (Product # 47-0247-42) in Flow Cytometry

Thu Jul 05 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); FLJ22950; FLJ43543; MGC75043; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

Gene Aliases: CD24; CD24A

UniProt ID: (Human) P25063

Entrez Gene ID: (Human) 100133941

Function(s)