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Invitrogen
CD249 (BP-1) Monoclonal Antibody (6C3), FITC, eBioscience™
-Flow-20170216130641.jpg?time=20220826 "CD249 (BP-1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 2
CD249 (BP-1) Antibody (11-5891-82) in Flow
Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) (Product # 12-0452-82) and 0.5 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse BP-1 FITC (right). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD249 (BP-1) Antibody (11-5891-82) in Flow")
Product Details
11-5891-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
6C3
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_465295
Product Specific Information
Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.
Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aminopeptidase A; aminopeptidase-A; AP-A; BP-1/6C3 antigen; BP1; CD249; EAP; Glutamyl aminopeptidase
Gene Aliases: 6030431M22Rik; APA; Bp-1/6C3; Enpep; Ly-51; Ly51
UniProt ID: (Mouse) P16406
Entrez Gene ID: (Mouse) 13809
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