Invitrogen

CD25 Monoclonal Antibody (BC96), eFluor™ 450, eBioscience™

Product Image Gallery

CD25 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and of Mouse IgG1 kappa Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD25 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Details

48-0259-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 27 publications 27 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	BC96
Conjugate	eFluor™ 450 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 615 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 711 FITC NovaFluor Red 685 NovaFluor Red 700 NovaFluor Yellow 700 PE PE-Cyanine5 PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 PerCP-eFluor 710 Super Bright
Excitation/Emission Max	405/445 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1834362

Product Specific Information

Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.

Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Identification of celastrol as a novel HIV-1 latency reversal agent by an image-based screen.

PloS one

Liu H,Hu PW,Dubrulle J,Stossi F,Nikolai BC,Mancini MA,Rice AP

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Tue Sep 14 00:00:00 EDT 2021

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Soluble fibrinogen‑like protein 2 levels are decreased in patients with ischemic heart failure and associated with cardiac function.

Molecular medicine reports

You Y,Huang S,Liu H,Fan C,Liu K,Wang Z

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.

Molecular therapy. Nucleic acids

Chang GP,Yang XL,Liu W,Lin S,Yang SL,Zhao MY

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9.

Scientific reports

Jarrell JA,Sytsma BJ,Wilson LH,Pan FL,Lau KHWJ,Kirby GTS,Lievano AA,Pawell RS

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Thu Jun 03 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Functionally specialized human CD4⁺ T-cell subsets express physicochemically distinct TCRs.

eLife

Kasatskaya SA,Ladell K,Egorov ES,Miners KL,Davydov AN,Metsger M,Staroverov DB,Matveyshina EK,Shagina IA,Mamedov IZ,Izraelson M,Shelyakin PV,Britanova OV,Price DA,Chudakov DM

48-0259-42 was used in Flow Cytometry to show that cumulative repertoire portraits establish a link between clonotype fate decisions in the complex world of CD4+ T cells and the intrinsic properties of somatically rearranged TCRs.

Tue Dec 08 00:00:00 EST 2020

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Potential of neutrophil to lymphocyte ratio in predicting sustained remission in rheumatoid arthritis compared to other immune activation markers.

The Indian journal of medical research

Chandrashekara S,Lingaraju DC,Renuka P,Anupama KR

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Dominant extrafollicular B cell responses in severe COVID-19 disease correlate with robust viral-specific antibody production but poor clinical outcomes.

medRxiv : the preprint server for health sciences

Woodruff M,Ramonell R,Cashman K,Nguyen D,Saini A,Haddad N,Ley A,Kyu S,Howell JC,Ozturk T,Lee S,Chen W,Estrada J,Morrison-Porter A,Derrico A,Anam F,Sharma M,Wu H,Le S,Jenks S,Tipton CM,Hu W,Lee FE,Sanz I

48-0259 was used in Flow cytometry/Cell sorting to find that critically-ill patients with COVID-19 robustly upregulate constituents of the extrafollicular pathway, produce enormous numbers of antibody secreting cells, and lose unique transitional B cell populations that correlate with positive prognosis.

Mon Jun 22 00:00:00 EDT 2020

Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.

JCI insight

Henderson LA,Hoyt KJ,Lee PY,Rao DA,Jonsson AH,Nguyen JP,Rutherford K,Julé AM,Charbonnier LM,Case S,Chang MH,Cohen EM,Dedeoglu F,Fuhlbrigge RC,Halyabar O,Hazen MM,Janssen E,Kim S,Lo J,Lo MS,Meidan E,Son MBF,Sundel RP,Stoll ML,Nusbaum C,Lederer JA,Chatila TA,Nigrovic PA

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

The Changing Landscape of Naive T Cell Receptor Repertoire With Human Aging.

Frontiers in immunology

Egorov ES,Kasatskaya SA,Zubov VN,Izraelson M,Nakonechnaya TO,Staroverov DB,Angius A,Cucca F,Mamedov IZ,Rosati E,Franke A,Shugay M,Pogorelyy MV,Chudakov DM,Britanova OV

48-0259 was used in Flow cytometry/Cell sorting to study the differences in the architecture of TCR beta repertoires with human aging.

Wed Nov 20 00:00:00 EST 2019

Transcriptional down-regulation of ccr5 in a subset of HIV+ controllers and their family members.

eLife

Gonzalo-Gil E,Rapuano PB,Ikediobi U,Leibowitz R,Mehta S,Coskun AK,Porterfield JZ,Lampkin TD,Marconi VC,Rimland D,Walker BD,Deeks S,Sutton RE

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Tue Apr 09 00:00:00 EDT 2019

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

A probiotic modulates the microbiome and immunity in multiple sclerosis.

Annals of neurology

Tankou SK,Regev K,Healy BC,Tjon E,Laghi L,Cox LM,Kivisäkk P,Pierre IV,Hrishikesh L,Gandhi R,Cook S,Glanz B,Stankiewicz J,Weiner HL

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.

Scientific reports

Albert BJ,Niu A,Ramani R,Marshall GR,Wender PA,Williams RM,Ratner L,Barnes AB,Kyei GB

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

Alteration of Th17 and Foxp3⁺ regulatory T cells in patients with unexplained recurrent spontaneous abortion before and after the therapy of hCG combined with immunoglobulin.

Experimental and therapeutic medicine

Sha J,Liu F,Zhai J,Liu X,Zhang Q,Zhang B

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

48-0259 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 EST 2017

Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.

Experimental and therapeutic medicine

Zhao J,Ma J,Zhang X,Li Q,Yang X

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.

The Journal of clinical investigation

MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using CD25 monoclonal antibody (Product # 48-0259-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; sIL 2R; soluble IL 2 receptor; TAC antigen

Gene Aliases: CD25; IDDM10; IL2R; IL2RA; IMD41; p55; TCGFR

UniProt ID: (Human) P01589

Entrez Gene ID: (Human) 3559

Molecular Function: transmembrane signal receptor

Function(s)

MAPK cascade inflammatory response to antigenic stimulus regulation of T cell tolerance induction apoptotic process activation-induced cell death of T cells inflammatory response immune response cell surface receptor signaling pathway Notch signaling pathway cell proliferation interleukin-2-mediated signaling pathway positive regulation of activated T cell proliferation negative regulation of T cell proliferation positive regulation of GTPase activity positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response

Process(es)

interleukin-2 receptor activity Ras guanyl-nucleotide exchange factor activity drug binding interleukin-2 binding

CD25 Monoclonal Antibody (BC96), eFluor™ 450, eBioscience™

Product Details

48-0259-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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