|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Analyte Specific Reagent
Helicobacter pylori induces in-vivo expansion of human regulatory T cells through stimulating interleukin-1ß production by dendritic cells.
MHCD2504-4 was used in flow cytometry to elucidate the influence of H. pylori on T(reg) function and proliferation.
|Mitchell PJ,Afzali B,Fazekasova H,Chen D,Ali N,Powell N,Lord GM,Lechler RI,Lombardi G||Clinical and experimental immunology (170:300)||2012|
Efficient and reproducible large-scale isolation of human CD4+ CD25+ regulatory T cells with potent suppressor activity.
MHCD2504-4 was used in flow cytometry to discuss methods to purify human CD4+ CD25+ regulatory T cells.
|Wichlan DG,Roddam PL,Eldridge P,Handgretinger R,Riberdy JM||Journal of immunological methods (315:27)||2006|
Human CD4+CD25+ regulatory cells have marked and sustained effects on CD8+ T cell activation.
MHCD2504-4 was used in flow cytometry to study the interactions between CD4(+)CD25(+) T cells and human CD8(+) T cells.
|Câmara NO,Sebille F,Lechler RI||European journal of immunology (33:3473)||2003|
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
MHCD2504-4 was used in flow cytometry to characterize CD4(+)CD25(+) human T cells.
|Ng WF,Duggan PJ,Ponchel F,Matarese G,Lombardi G,Edwards AD,Isaacs JD,Lechler RI||Blood (98:2736)||2001|
In vivo inhibition of human CD19-targeted effector T cells by natural T regulatory cells in a xenotransplant murine model of B cell malignancy.
MHCD2504-4 was used in flow cytometry to examine the impact of Tregs on CAR-modified T cells in the SCID-Beige xenotransplant model.
|Lee JC,Hayman E,Pegram HJ,Santos E,Heller G,Sadelain M,Brentjens R||Cancer research (71:2871)||2011|