Immunofluorescent analysis of CD25 (green) showing staining in the cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD25 monoclonal antibody (Product # MA5-12680) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human interleukin-2 receptor|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12680 targets CD25 in FACS, IHC (P) and IF applications and shows reactivity with Human and Mouse samples.
The MA5-12680 immunogen is recombinant human interleukin-2 receptor.
IL-2R (T-cell growth factor receptor) consists of a chain of 55kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2Ralpha is over-expressed on the surface of activated T-cells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages.
IP-MS enrichment of IL2RA (LFQ intensity): IL2RA was enriched 571-fold from SR lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and IL2RA antibody (Part No. 710390). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD25 is a novel marker of hepatic bile canaliculus.
MA5-12680 was used in immunohistochemistry to study CD25 as a new marker of bile canaliculus
|Lu ZH,-Chen W,Ju CX,-Den J,Kuai SG,Pei H,Huang LH,Gu XB,Ying YX,Hu XQ,Zhu TF,Xing YP,Fan QH||International journal of surgical pathology (20:455)||2012|
FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity.
MA5-12680 was used in immunohistochemistry to investigate the influence of FOXP3 T regulatory cells in the pathogenesis of oral lichen planus
|Tao XA,Xia J,Chen XB,Wang H,Dai YH,Rhodus NL,Cheng B||Oral diseases (16:76)||2010|
Regulatory T cells and liver pathology in a murine graft versus host response model.
MA5-12680 was used in immunohistochemistry to study regulatory T cell activity and liver pathology in a mouse model of graft-versus-host response
|Miyazaki T,Doy M,Unno R,Honda A,Ikegami T,Itoh S,Bouscarel B,Matsuzaki Y||Hepatology research : the official journal of the Japan Society of Hepatology (39:585)||2009|
Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury.
MA5-12680 was used in immunohistochemistry to generate a mouse model of acquired glomerular sclerosis
|Matsusaka T,Xin J,Niwa S,Kobayashi K,Akatsuka A,Hashizume H,Wang QC,Pastan I,Fogo AB,Ichikawa I||Journal of the American Society of Nephrology : JASN (16:1013)||2005|
Adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures via recruitment of p120 catenin to E-cadherin.
MA5-12680 was used in western blot to study the mechanism by which the adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures
|Tricaud N,Perrin-Tricaud C,Brusés JL,Rutishauser U||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:3259)||2005|
Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.
MA5-12680 was used in western blot to investigate the involvement of beta3 integrin and c-Src in focal adhesion complex formation in adult cardiomyocytes
|Willey CD,Balasubramanian S,Rodríguez Rosas MC,Ross RS,Kuppuswamy D||Journal of molecular and cellular cardiology (35:671)||2003|
Association of increased levels of heavy-chain ferritin with increased CD4+ CD25+ regulatory T-cell levels in patients with melanoma.
MA5-12680 was used in flow cytometry to study the association between increased levels of heavy-chain ferritin and increased CD4+ CD25+ regulatory T-cell levels in patients with melanoma
|Gray CP,Arosio P,Hersey P||Clinical cancer research : an official journal of the American Association for Cancer Research (9:2551)||2003|