Invitrogen

CD25 Monoclonal Antibody (IL2R.1)

Advanced Verification

IP-mass spectrometry

13 References

View all (238) CD25 antibodies

Datasheet

Protocols

Datasheet

Protocols

Antibody Testing Data (5)
Advanced Verification (1)

CD25 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CD25 Antibody (MA5-12680) in ICC/IF

Immunofluorescent analysis of CD25 (green) showing staining in the cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD25 monoclonal antibody (Product # MA5-12680) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugate... View More

CD25 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CD25 Antibody — IP-MS enrichment of IL2RA (LFQ intensity): IL2RA was enriched 244-fold from SR lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and IL2RA antibody (Product # MA5-12680). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

MA5-12680

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

IL2R.1

Immunogen

Recombinant human interleukin-2 receptor

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979943

Product Specific Information

MA5-12680 targets CD25 in FACS, IHC (P) and IF applications and shows reactivity with Human and Mouse samples.^M
^M
The MA5-12680 immunogen is recombinant human interleukin-2 receptor.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization and Optimization of the Tumor Microenvironment in Patient-Derived Organotypic Slices and Organoid Models of Glioblastoma.

Cancers

Nickl V,Eck J,Goedert N,Hübner J,Nerreter T,Hagemann C,Ernestus RI,Schulz T,Nickl RC,Keßler AF,Löhr M,Rosenwald A,Breun M,Monoranu CM

MA5-12680 was used in Immunohistochemistry to present an analysis of tumor microenvironment (TME) composition in a patient-derived organoid model (PDO) as well as in organotypic slice cultures (OSC).

Wed May 10 00:00:00 UTC 2023

Autologous olfactory mucosa mesenchymal stem cells treatment improves the neural network in chronic refractory epilepsy.

Stem cell research & therapy

Liu ZZ,Huang Y,Hong CG,Wang X,Duan R,Liu JY,He JL,Duan D,Xie H,Lu M

MA5-12680 was used in Immunohistochemistry to reveal that OM-MSC treatment recruited Treg cells to the brain, inhibited inflammation, rebuilt the neural network, and improved the cognitive, locomotive, and perceptive functions of epileptic mice.

Thu Sep 07 00:00:00 UTC 2023

Effects of life-stage and passive tobacco smoke exposure on pulmonary innate immunity and influenza infection in mice.

Journal of toxicology and environmental health. Part A

Wang L,Rajavel M,Wu CW,Zhang C,Poindexter M,Fulgar C,Mar T,Singh J,Dhillon JK,Zhang J,Yuan Y,Abarca R,Li W,Pinkerton KE

MA5-12680 was used in Immunohistochemistry to suggest perinatal ETS exposure induces an exaggerated innate immune response, which may overwhelm protective anti-inflammatory defenses against IAV, and enhances severity of infection at early life stages (e.g., in infants and young children).

Fri Jun 03 00:00:00 UTC 2022

Modulation of PTPN2/22 Function by Spermidine in CRISPR-Cas9-Edited T-Cells Associated with Crohn's Disease and Rheumatoid Arthritis.

International journal of molecular sciences

Shaw AM,Qasem A,Naser SA

Published figure using CD25 monoclonal antibody (Product # MA5-12680) in Immunocytochemistry

Wed Aug 18 00:00:00 UTC 2021

Modulation of PTPN2/22 Function by Spermidine in CRISPR-Cas9-Edited T-Cells Associated with Crohn's Disease and Rheumatoid Arthritis.

International journal of molecular sciences

Shaw AM,Qasem A,Naser SA

Published figure using CD25 monoclonal antibody (Product # MA5-12680) in Immunocytochemistry

Wed Aug 18 00:00:00 UTC 2021

Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.

Cancer cell

Costa A,Kieffer Y,Scholer-Dahirel A,Pelon F,Bourachot B,Cardon M,Sirven P,Magagna I,Fuhrmann L,Bernard C,Bonneau C,Kondratova M,Kuperstein I,Zinovyev A,Givel AM,Parrini MC,Soumelis V,Vincent-Salomon A,Mechta-Grigoriou F

MA5-12680 was used in Immunohistochemistry-immunofluorescence to study the role of carcinoma associated fibroblasts in the tumour microenvironment.

Mon Mar 12 00:00:00 UTC 2018

A kinetic investigation of interacting, stimulated T cells identifies conditions for rapid functional enhancement, minimal phenotype differentiation, and improved adoptive cell transfer tumor eradication.

PloS one

Zhou J,Bethune MT,Malkova N,Sutherland AM,Comin-Anduix B,Su Y,Baltimore D,Ribas A,Heath JR

MA5-12680 was used in Immunohistochemistry to investigate how the short-term kinetics of T cell functional activation, through molecular stimulation and cell-cell interactions, competes with phenotype differentiation.

Mon Feb 26 00:00:00 UTC 2018

CD25 is a novel marker of hepatic bile canaliculus.

International journal of surgical pathology

Lu ZH,-Chen W,Ju CX,-Den J,Kuai SG,Pei H,Huang LH,Gu XB,Ying YX,Hu XQ,Zhu TF,Xing YP,Fan QH

MA5-12680 was used in immunohistochemistry to study CD25 as a new marker of bile canaliculus

Mon Oct 01 00:00:00 UTC 2012

FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity.

Oral diseases

Tao XA,Xia J,Chen XB,Wang H,Dai YH,Rhodus NL,Cheng B

MA5-12680 was used in immunohistochemistry to investigate the influence of FOXP3 T regulatory cells in the pathogenesis of oral lichen planus

Fri Jan 01 00:00:00 UTC 2010

Regulatory T cells and liver pathology in a murine graft versus host response model.

Hepatology research : the official journal of the Japan Society of Hepatology

Miyazaki T,Doy M,Unno R,Honda A,Ikegami T,Itoh S,Bouscarel B,Matsuzaki Y

MA5-12680 was used in immunohistochemistry to study regulatory T cell activity and liver pathology in a mouse model of graft-versus-host response

Mon Jun 01 00:00:00 UTC 2009

Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury.

Journal of the American Society of Nephrology : JASN

Matsusaka T,Xin J,Niwa S,Kobayashi K,Akatsuka A,Hashizume H,Wang QC,Pastan I,Fogo AB,Ichikawa I

MA5-12680 was used in immunohistochemistry to generate a mouse model of acquired glomerular sclerosis

Fri Apr 01 00:00:00 UTC 2005

Adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures via recruitment of p120 catenin to E-cadherin.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tricaud N,Perrin-Tricaud C,Brusés JL,Rutishauser U

MA5-12680 was used in western blot to study the mechanism by which the adherens junctions in myelinating Schwann cells stabilize Schmidt-Lanterman incisures

Wed Mar 30 00:00:00 UTC 2005

Association of increased levels of heavy-chain ferritin with increased CD4+ CD25+ regulatory T-cell levels in patients with melanoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gray CP,Arosio P,Hersey P

MA5-12680 was used in flow cytometry to study the association between increased levels of heavy-chain ferritin and increased CD4+ CD25+ regulatory T-cell levels in patients with melanoma

Tue Jul 01 00:00:00 UTC 2003

Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.

Journal of molecular and cellular cardiology

Willey CD,Balasubramanian S,Rodríguez Rosas MC,Ross RS,Kuppuswamy D

MA5-12680 was used in western blot to investigate the involvement of beta3 integrin and c-Src in focal adhesion complex formation in adult cardiomyocytes

Sun Jun 01 00:00:00 UTC 2003

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more