Invitrogen

CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), APC, eBioscience™

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Antibody Testing Data (1)
Published Figures (6)

CD252 (OX40 Ligand) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD252 (OX40 Ligand) Antibody (17-5905-82) in Flow

Mouse splenocytes were stimulated for 4 days with F (ab')2 Anti-Mouse IgM, u chain specific and Anti-Mouse CD40 Functional Grade Purifieds (Product # 16-5092-85 and Product # 16-0401-82). Cells were stained with 0.25 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (blue histogram) or 0.25 µg of Anti-Mouse CD252 (OX40 Ligand) APC (purple histogram). Total viable cells, as determined by Fixable V... View More

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Product Details

17-5905-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RM134L

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11044244

Product Specific Information

Description: The RM134L monoclonal antibody reacts with mouse CD252 also known as OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.

Applications Reported: This RM134L antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RM134L antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Regulatory T Cell-Derived TGF-β1 Controls Multiple Checkpoints Governing Allergy and Autoimmunity.

Immunity

Turner JA,Stephen-Victor E,Wang S,Rivas MN,Abdel-Gadir A,Harb H,Cui Y,Fanny M,Charbonnier LM,Fong JJH,Benamar M,Wang L,Burton OT,Bansal K,Bry L,Zhu C,Li QZ,Clement RL,Oettgen HC,Crestani E,Rachid R,Sage PT,Chatila TA

17-5905-82 was used in Flow Cytometry to show that Treg cells in food allergy (FA) had decreased expression of transforming growth factor beta 1 (TGF-β1) because of interleukin-4 (IL-4)- and signal transducer and activator of transciription-6 (STAT6)-dependent inhibition of Tgfb1 transcription.

Tue Dec 15 00:00:00 EST 2020

Localized Treatment with Oncolytic Adenovirus Delta-24-RGDOX Induces Systemic Immunity against Disseminated Subcutaneous and Intracranial Melanomas.

Clinical cancer research : an official journal of the American Association for Cancer Research

Jiang H,Shin DH,Nguyen TT,Fueyo J,Fan X,Henry V,Carrillo CC,Yi Y,Alonso MM,Collier TL,Yuan Y,Lang FF,Gomez-Manzano C

17-5905 was used in Flow cytometry/Cell sorting to show that localized intratumoral injection of Delta-24-RGDOX induces an in situ antovaccination of the treated melanoma, the effect of which changes the immune landscape of the treated mice, resulting in systemic immunity against disseminated s.c. and i.c. tumors.

Fri Nov 15 00:00:00 EST 2019

Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells.

Scientific reports

Knox T,Sahakian E,Banik D,Hadley M,Palmer E,Noonepalle S,Kim J,Powers J,Gracia-Hernandez M,Oliveira V,Cheng F,Chen J,Barinka C,Pinilla-Ibarz J,Lee NH,Kozikowski A,Villagra A

Published figure using CD252 (OX40 Ligand) monoclonal antibody (Product # 17-5905-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Bovine κ-Casein Fragment Induces Hypo-Responsive M2-Like Macrophage Phenotype.

Nutrients

Lalor R,O'Neill S

Published figure using CD252 (OX40 Ligand) monoclonal antibody (Product # 17-5905-82) in Flow Cytometry

Tue Jul 23 00:00:00 EDT 2019

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

17-5905 was used in Flow cytometry/Cell sorting to demonstrate that combining an oncolytic virus with tumor-targeting immune checkpoint modulators elicits potent in situ autologous cancer vaccination, resulting in an efficacious, tumor-specific, and long-lasting therapeutic effect.

Sat Jul 15 00:00:00 EDT 2017

Curcumin improves regulatory T cells in gut-associated lymphoid tissue of colitis mice.

World journal of gastroenterology

Zhao HM,Xu R,Huang XY,Cheng SM,Huang MF,Yue HY,Wang X,Zou Y,Lu AP,Liu DY

17-5905 was used in Flow cytometry/Cell sorting to investigate the pathway by which curcumin regulates the function of Tregs.

Tue Jun 21 00:00:00 EDT 2016

CD4+ T cell responses in Balb/c mice with food allergy induced by trinitrobenzene sulfonic acid and ovalbumin.

Molecular medicine reports

Sun CY,Bai J,Hu TY,Cheng BH,Ma L,Fan XQ,Yang PC,Zheng PY,Liu ZQ

17-5905-82 was used in Flow Cytometry to suggest that TNBS, as a hapten, combined with food antigens may lead to a Th2 cell response in the intestinal mucosa.

Wed Jun 01 00:00:00 EDT 2016

Dectin-1-activated dendritic cells trigger potent antitumour immunity through the induction of Th9 cells.

Nature communications

Zhao Y,Chu X,Chen J,Wang Y,Gao S,Jiang Y,Zhu X,Tan G,Zhao W,Yi H,Xu H,Ma X,Lu Y,Yi Q,Wang S

Published figure using CD252 (OX40 Ligand) monoclonal antibody (Product # 17-5905-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2016

Neutralization of TSLP inhibits airway remodeling in a murine model of allergic asthma induced by chronic exposure to house dust mite.

PloS one

Chen ZG,Zhang TT,Li HT,Chen FH,Zou XL,Ji JZ,Chen H

Published figure using CD252 (OX40 Ligand) monoclonal antibody (Product # 17-5905-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: atherosclerosis 1; CD252; OX40 ligand; OX40L; tax-transcriptionally activated glycoprotein 1 ligand; Tumor necrosis factor ligand superfamily member 4

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Gene Aliases: Ath-1; Ath1; CD134L; gp34; OX-40L; Ox40l; Tnfsf4; TXGP1; Txgp1l

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UniProt ID: (Mouse) P43488

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Entrez Gene ID: (Mouse) 22164

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Function(s)

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

Process(es)

Performance Guarantee

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CD252 (OX40 Ligand) Monoclonal Antibody (RM134L), APC, eBioscience™

CD252 (OX40 Ligand) Antibody (17-5905-82) in Flow

Product Details

17-5905-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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