|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The monoclonal antibody H194-112 recognizes mouse CD26, a thymocyte-activating molecule (THAM). CD26 is a surface expressed and soluble ectoenzyme (dipeptidyl peptidase IV) that cleaves dipeptides from the amino terminus of proteins. In particular CD26 has been shown to cleave CXCL12, CCL22, neuropeptide Y and glucagon. CD26 is involved in adhesion, apoptosis, disease regulation, and engraftment. Expression is regulated during thymic development at the messenger RNA level. Additionally, CD26 protein is found on thymocytes, a percentage of resting B cells, stimulated B cells, NK cells and a percentage of resting and activated T cells and other epithelial cell types. On human cells more CD26 protein is found on Th2 cells. Functional CD26 is expressed on Sca-1+c-Kit+lin- cells. This expression has been shown to correlate with mobilization of progenitor cells. Inhibition of CD26 activity through a variety of compounds enhances allogenic stem cell homing and engraftment.
This antibody is reported to have activating function.
Applications Reported: This H194-112 (H194-184.108.40.206.5) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This H194-112 (H194-220.127.116.11.5) antibody has been tested by flow cytometric analysis of mouse splenocytes or thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
CD26 [also known as dipeptidyl peptidase IV (DPP IV), adenosine deaminase (ADA) binding protein]. 1 - 3 is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti CD3-induced IL - 2 production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||The multifunctional or moonlighting protein CD26/DPPIV.||Boonacker E,Van Noorden CJ||European journal of cell biology (82:53)||2003|
|Not Applicable||Not Cited||cDNA cloning for mouse thymocyte-activating molecule. A multifunctional ecto-dipeptidyl peptidase IV (CD26) included in a subgroup of serine proteases.||Marguet D,Bernard AM,Vivier I,Darmoul D,Naquet P,Pierres M||The Journal of biological chemistry (267:2200)||1992|
|Not Applicable||Not Cited||Activation of mouse T lymphocytes by a monoclonal antibody to a developmentally regulated surface aminopeptidase (THAM).||Naquet P,Vivier I,Gorvel JP,Brekelmans P,Barad M,Bernard AM,Pierres M||Immunological reviews (111:177)||1989|