Flow cytometry analysis of CD26 in PBMC cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA5-13562) at a dilution of 0.25 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Human T cell clone|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13562 targets CD26 in FACS and IF applications and shows reactivity with Human and Rat samples. This antibody is not suitable for rat PC12 cells in FACS analysis.
The MA5-13562 immunogen is human T cell clone.
CD26 [also known as dipeptidyl peptidase IV (DPP IV), adenosine deaminase (ADA)-binding protein]. is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Co-purification of Mac-2 binding protein with galectin-3 and association with prostasomes in human semen.
MA5-13562 was used in western blot to identify the candidate binding ligands of galectin-3 in human semen
|Block AS,Saraswati S,Lichti CF,Mahadevan M,Diekman AB||The Prostate (71:711)||2011|
Heparan sulfate plays a central role in a dynamic in vitro model of protein-losing enteropathy.
MA5-13562 was used in immunohistochemistry To study the the role of heparan sulfate in a dynamic in vitro model of protein-losing enteropathy
|Bode L,Murch S,Freeze HH||The Journal of biological chemistry (281:7809)||2006|