|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.
Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
CD27 (50 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily. CD40 and CD30 are also members of the TNF receptor superfamily. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is a membranebound receptor, but a soluble form of CD27 is also produced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Characterization of murine CD70, the ligand of the TNF receptor family member CD27.||Tesselaar K,Gravestein LA,van Schijndel GM,Borst J,van Lier RA||Journal of immunology (Baltimore, Md. : 1950) (159:4959)||1997|
|Not Applicable||Not Cited||Characterization of murine CD70, the ligand of the TNF receptor family member CD27.||Tesselaar K,Gravestein LA,van Schijndel GM,Borst J,van Lier RA||Journal of immunology (Baltimore, Md. : 1950) (159:4959)||1997|