|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant human CD27 extracellular domain, expressed in insect Sf21 cells.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Flow Cytometry (Flow)||0.5-1 µg for 10^6 cells|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24876 detects CD27 from human samples.
PA1-24876 has been successfully used in flow cytometry, and Western blot procedures.
The PA1-24876 immunogen is recombinant human CD27 extracellular domain, expressed in insect Sf21 cells.
Store at 4°C short term. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.