Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), APC, eBioscience™

Product Image Gallery

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow) — Staining of unstimulated (left) or PHA-stimulated (right) normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD274 (B7-H1) APC (purple histogram). Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 520 (Product # 65-0867-14), were used for analysis.

Product Details

17-5983-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.5 µg)/test	View 21 publications 21 publications
Western Blot (WB)	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Functional Assay (FN)	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MIH1
Conjugate	APC Alexa Fluor 488 Alexa Fluor 700 APC-eFluor 780 Biotin eFluor 450 Functional Grade PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	651/660 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10597586

Product Specific Information

Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.

Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

The Immunostimulative Effect and Mechanisms of a Novel Mouse Anti-Human PD-1 Monoclonal Antibody on Jurkat Lymphocytic Cells Cocultured with Hepatoma Cells.

OncoTargets and therapy

Li Z,Li B,Li L,Wang G,Li Y,Fu R,Ming Y,Ni R,Wang J,Ye G,Chen J

17-5983 was used in Western Blotting to demonstrated that anti-PD-1 mAb B1C4 could inhibit the apoptosis of Jurkat cells induced by HePG2 hepatoma cells and reverse the immunosuppressive effect of HePG2 cells on Jurkat cells.

Mon Apr 18 00:00:00 EDT 2022

A secondary role for hypoxia and HIF1 in the regulation of (IFNγ-induced) PD-L1 expression in melanoma.

Cancer immunology, immunotherapy : CII

van Duijn A,Willemsen KJ,van Uden NOP,Hoyng L,Erades S,Koster J,Luiten RM,Bakker WJ

17-5983-42 was used in Flow Cytometry to show the influence of hypoxia on IFNγ-induced PD-L1 expression, are relevant for immunotherapy, as both IFNγ and hypoxia are frequently present in the tumor microenvironment.

Tue Mar 01 00:00:00 EST 2022

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

17-5983-42 was used in Flow Cytometry to show the feasibility and efficacy of human MUC16 ectodomain- specific BiTEDs and provide a basis for the combination with anti-VEGF therapy for ovarian cancer.

Thu Oct 21 00:00:00 EDT 2021

T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer.

Oncoimmunology

O'Connor RA,Chauhan V,Mathieson L,Titmarsh H,Koppensteiner L,Young I,Tagliavini G,Dorward DA,Prost S,Dhaliwal K,Wallace WA,Akram AR

17-5983-42 was used in Flow Cytometry to study co-inhibitory ligands in T cell immunosuppression in non-small cell lung cancer.

Mon Aug 02 00:00:00 EDT 2021

CRISPR Screening of CAR T Cells and Cancer Stem Cells Reveals Critical Dependencies for Cell-Based Therapies.

Cancer discovery

Wang D,Prager BC,Gimple RC,Aguilar B,Alizadeh D,Tang H,Lv D,Starr R,Brito A,Wu Q,Kim LJY,Qiu Z,Lin P,Lorenzini MH,Badie B,Forman SJ,Xie Q,Brown CE,Rich JN

17-5983-42 was used in Flow Cytometry to interrogate molecular determinants of CAR-mediated GBM killing through whole-genome CRISPR screens in both CAR T cells and patient-derived GSCs.

Sat May 01 00:00:00 EDT 2021

LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer.

Cell death & disease

Xu S,Wang X,Yang Y,Li Y,Wu S

17-5983-42 was used in Flow Cytometry to validate the increase in expression of LSD1 during the progression of cervical cancer and confirmed the enhanced therapeutic effect against cervical cancer when combining CD47/PD-L1 blockade therapy with an LSD1 inhibitor.

Wed Mar 17 00:00:00 EDT 2021

Amplification of N-Myc is associated with a T-cell-poor microenvironment in metastatic neuroblastoma restraining interferon pathway activity and chemokine expression.

Oncoimmunology

Layer JP,Kronmüller MT,Quast T,van den Boorn-Konijnenberg D,Effern M,Hinze D,Althoff K,Schramm A,Westermann F,Peifer M,Hartmann G,Tüting T,Kolanus W,Fischer M,Schulte J,Hölzel M

17-5983 was used in Flow cytometry to investigate the molecular characteristics of T-cell infiltration in primary neuroblastomas as an indicator of pre-existing immune responses and potential responsiveness to checkpoint inhibition.

Tue Jan 12 00:00:00 EST 2021

Synergistic inhibition of pancreatic cancer with anti-PD-L1 and c-Myc inhibitor JQ1.

Oncoimmunology

Pan Y,Fei Q,Xiong P,Yang J,Zhang Z,Lin X,Pan M,Lu F,Huang H

17-5983-42 was used in Flow Cytometry to demonstrate that the expression of PD-L1 and c-Myc may be helpful prognostic biomarkers, and their inhibition may potentially serve as an effective treatment for PDAC.

Tue Jan 12 00:00:00 EST 2021

HGF-Induced PD-L1 Expression in Head and Neck Cancer: Preclinical and Clinical Findings.

International journal of molecular sciences

Boschert V,Teusch J,Aljasem A,Schmucker P,Klenk N,Straub A,Bittrich M,Seher A,Linz C,Müller-Richter UDA,Hartmann S

17-5983 was used in Flow cytometry/Cell sorting to indicate a potential role for sPD-L1 as a prognostic marker for immune checkpoint inhibitor treatment in head neck squamous cell carcinoma.

Fri Nov 20 00:00:00 EST 2020

Activate: Randomized Clinical Trial of BCG Vaccination against Infection in the Elderly.

Cell

Giamarellos-Bourboulis EJ,Tsilika M,Moorlag S,Antonakos N,Kotsaki A,Domínguez-Andrés J,Kyriazopoulou E,Gkavogianni T,Adami ME,Damoraki G,Koufargyris P,Karageorgos A,Bolanou A,Koenen H,van Crevel R,Droggiti DI,Renieris G,Papadopoulos A,Netea MG

17-5983-42 was used in Flow Cytometry to assess whether BCG has similar effects in the elderly in a phase III ACTIVATE trial.

Thu Oct 15 00:00:00 EDT 2020

CD44 Promotes PD-L1 Expression and Its Tumor-Intrinsic Function in Breast and Lung Cancers.

Cancer research

Kong T,Ahn R,Yang K,Zhu X,Fu Z,Morin G,Bramley R,Cliffe NC,Xue Y,Kuasne H,Li Q,Jung S,Gonzalez AV,Camilleri-Broet S,Guiot MC,Park M,Ursini-Siegel J,Huang S

17-5983 was used in Flow cytometry/Cell sorting to provide a novel basis for CD44 as a critical therapeutic target to suppress PD-L1 tumor-intrinsic function.

Sat Feb 01 00:00:00 EST 2020

Multidimensional analyses of proinsulin peptide-specific regulatory T cells induced by tolerogenic dendritic cells.

Journal of autoimmunity

Suwandi JS,Laban S,Vass K,Joosten A,van Unen V,Lelieveldt BPF,Höllt T,Zwaginga JJ,Nikolic T,Roep BO

17-5983-42 was used in Flow Cytometry to investigate if tolerogenic dendritic cells can induce antigen-specific T-cells.

Sat Feb 01 00:00:00 EST 2020

CA-170 - A Potent Small-Molecule PD-L1 Inhibitor or Not?

Molecules (Basel, Switzerland)

Musielak B,Kocik J,Skalniak L,Magiera-Mularz K,Sala D,Czub M,Stec M,Siedlar M,Holak TA,Plewka J

Thu Aug 01 00:00:00 EDT 2019

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids.

Cell

Dijkstra KK,Cattaneo CM,Weeber F,Chalabi M,van de Haar J,Fanchi LF,Slagter M,van der Velden DL,Kaing S,Kelderman S,van Rooij N,van Leerdam ME,Depla A,Smit EF,Hartemink KJ,de Groot R,Wolkers MC,Sachs N,Snaebjornsson P,Monkhorst K,Haanen J,Clevers H,Schumacher TN,Voest EE

17-5983-42 was used in Flow Cytometry to describe a platform to induce and analyze tumor-specific T cell responses to epithelial cancers in a personalized manner.

Thu Sep 06 00:00:00 EDT 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

17-5983 was used in Flow cytometry/Cell sorting to suggest that despite the fact that helminth parasites and cancer cell lines are extraordinarily disparate, they share the ability to alter the phenotype of human monocytes.

Sun Apr 01 00:00:00 EDT 2018

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

17-5983 was used in Flow cytometry/Cell sorting to demonstrate that combining an oncolytic virus with tumor-targeting immune checkpoint modulators elicits potent in situ autologous cancer vaccination, resulting in an efficacious, tumor-specific, and long-lasting therapeutic effect.

Sat Jul 15 00:00:00 EDT 2017

Correlation of PD-L1 Surface Expression on Leukemia Cells with the Ratio of PD-L1 mRNA Variants and with Electrophoretic Mobility.

Cancer immunology research

Brodská B,Otevřelová P,Kuželová K

17-5983 was used in Western blot to show that the expression of PD-L1 on the surface of leukemic and normal hematopoietic cells correlates with the ratio of mRNA variant 1 to variant 2.

Sat Oct 01 00:00:00 EDT 2016

Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition.

The Journal of clinical investigation

Cherkassky L,Morello A,Villena-Vargas J,Feng Y,Dimitrov DS,Jones DR,Sadelain M,Adusumilli PS

17-5983 was used in Flow cytometry/Cell sorting to provide mechanistic insights into human chimeric antigen receptor T-cell exhaustion in solid tumors.

Mon Aug 01 00:00:00 EDT 2016

Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110.

PloS one

Deisting W,Raum T,Kufer P,Baeuerle PA,Münz M

17-5983 was used in Flow cytometry/Cell sorting to suggest that diverse mechanisms employed by cancer cells to fend off T-cells cannot inactivate AMG 110-engaged T-cells.

Thu Jun 16 00:00:00 EDT 2016

PDL1 Expression on Plasma and Dendritic Cells in Myeloma Bone Marrow Suggests Benefit of Targeted anti PD1-PDL1 Therapy.

PloS one

Sponaas AM,Moharrami NN,Feyzi E,Standal T,Holth Rustad E,Waage A,Sundan A

17-5983 was used in Flow cytometry/Cell sorting to investigate whether anti PDL1 or PD-1 treatment targeting the immune system could be used against multiple myeloma.

Fri Jun 03 00:00:00 EDT 2016

Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.

PloS one

Sommer A,Fabri M

17-5983 was used in Flow cytometry/Cell sorting to study the role of vitamin D in regulating dendritic cell-mediated immune responses in humans.

Tue Apr 26 00:00:00 EDT 2016

High and interrelated rates of PD-L1+CD14+ antigen-presenting cells and regulatory T cells mark the microenvironment of metastatic lymph nodes from patients with cervical cancer.

Cancer immunology research

Heeren AM,Koster BD,Samuels S,Ferns DM,Chondronasiou D,Kenter GG,Jordanova ES,de Gruijl TD

17-5983 was used in Immunocytochemistry-immunoflourescence to investigate the microenvironment of tumour-draining lymph nodes of patients with cervical cancer.

Thu Jan 01 00:00:00 EST 2015

HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.

Journal of virology

Planès R,BenMohamed L,Leghmari K,Delobel P,Izopet J,Bahraoui E

17-5983 was used in Functional assays to investigate the mechanisms by which HIV-1 induces upregulation of coinhibitory molecules, showing that the HIV-1 Tat protein induces PD-L1 expression on dendritic cells.

Sun Jun 01 00:00:00 EDT 2014

Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of lupus.

Journal of immunology (Baltimore, Md. : 1950)

Zhang L,Bertucci AM,Ramsey-Goldman R,Burt RK,Datta SK

17-5983 was used in Flow cytometry/Cell sorting to show that after hemopoietic stem cell posttransplant patients certain Treg populations return to levels seen in healthy patient.

Sun Nov 15 00:00:00 EST 2009

Major depletion of plasmacytoid dendritic cells in HIV-2 infection, an attenuated form of HIV disease.

PLoS pathogens

Cavaleiro R,Baptista AP,Soares RS,Tendeiro R,Foxall RB,Gomes P,Victorino RM,Sousa AE

17-5983 was used in Flow cytometry/Cell sorting to understand the contribution of plasmacytoid dendritic cell biology to HIV/AIDS pathogenesis.

Sun Nov 01 00:00:00 EDT 2009

Expression of B7-H1 in inflammatory renal tubular epithelial cells.

Nephron. Experimental nephrology

Chen Y,Zhang J,Li J,Zou L,Zhao T,Tang Y,Wu Y

Fri Apr 21 00:00:00 EDT 2006

Expression of B7-H1 in inflammatory renal tubular epithelial cells.

Nephron. Experimental nephrology

Chen Y,Zhang J,Li J,Zou L,Zhao T,Tang Y,Wu Y

Fri Apr 21 00:00:00 EDT 2006

Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ohigashi Y,Sho M,Yamada Y,Tsurui Y,Hamada K,Ikeda N,Mizuno T,Yoriki R,Kashizuka H,Yane K,Tsushima F,Otsuki N,Yagita H,Azuma M,Nakajima Y

Fri Apr 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

Gene Aliases: B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1

UniProt ID: (Human) Q9NZQ7

Entrez Gene ID: (Human) 29126

Molecular Function: immunoglobulin receptor superfamily

Function(s)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production response to cytokine positive regulation of T cell proliferation regulation of activated T cell proliferation negative regulation of activated T cell proliferation regulation of T cell apoptotic process toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

Process(es)

protein binding

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), APC, eBioscience™

Product Details

17-5983-42

Flow Cytometry (Flow)

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Functional Assay (FN)

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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