Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), eBioscience™

Product Image Gallery

CD274 (PD-L1, B7-H1) Antibody — Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 14-5982-82)

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.25 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (left) or 0.25 µg of Anti-Mouse CD274 (PD-L1, B7-H1) Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.25 µg/test

Functional Assay (FN)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

Western Blot (WB)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 38 publications below

Immunohistochemistry (IHC)

See 5 publications below

Western Blot (WB)

See 2 publications below

Immunofluorescence (IF)

See 3 publications below

Neutralization (Neu)

See 9 publications below

Functional Assay (FN)

See 2 publications below

Inhibition Assays (IA)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG2a, lambda

Class

Monoclonal

Type

Antibody

Clone

MIH5

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467781

Product Specific Information

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking in in vitro and in vivo functional assays. (Please use Functional Grade Purified MIH5, cat. 16-5982, in functional assays. Fluorochrome-conjugated MIH5 is recommended for use in flow cytometry.).

Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H; B7-H1; B7H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; programmed death ligand 1

Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1

UniProt ID: (Mouse) Q9EP73

Entrez Gene ID: (Mouse) 60533

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily membrane-bound signaling molecule receptor signaling molecule