Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

26 Published Figures

24 References

View all (169) PD-L1 (CD274) antibodies

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Protocols

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CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with Anti-Mouse CD3 eFluor® 450 (Product # 48-0032-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse CD274 (B7-H1) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

CD274 (PD-L1, B7-H1) Antibody — Figure 3 Open in a separate window Induction of PD-L1 in NDV-treated and distant tumors. ( A ) mRNA expression of PDL1 and PDL2 in the cultured NDV-infected tumor specimens (48 h) and NDV-infected whole blood (24 h) obtained from healthy donors and from patients with cancer. ( B ) Mouse treatment schema. Tumors were collected at 24 hours (early) or 6 days (late) after the first treatment. ( C and D ) Upregulation of PD-L1 on CD45 + and CD45 - cells in treated tumors ( C ) and distant tumors ( D ) at 24 hours (early). Left: representative flow cytometry histograms; right: quantified PD-L1 MFI. ( E and F ) Upregulation of PD-L1 on CD45 + and CD45 - cells in treated tumors ( E ) and distant tumors ( F ) on day 6 (late). Shown are representative flow cytometric histograms and quantification of PD-L1 MFI on CD45 - cells and on the indicated leukocyte subsets. ( G ) Expression of PD-L1 in distant tumors on day 6. ( H ) MFI of PD-L1 expression in GFP - and GFP + CD45 + cells isolated from the tumors treated with NDV expressing GFP 24 hours after infection. Scale bars: 500 mum and 50 mum (enlarged insets). ( A ) Each specimen represents an individual experiment. ( B - H ) Results are representative of 3 independent experiments with 3 to 5 animals per group, and data represent the mean +- SEM. Data were analyzed by a Wilcoxon matched-pairs, signed-rank test ( A ) and a Student's t test for individual comparisons ( C - F and H ). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0 {TM}

Product Details

25-5982-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.125 µg/test	View 24 publications 24 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, lambda
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MIH5
Conjugate	PE-Cyanine7 Alexa Fluor 488 Alexa Fluor 660 APC Biotin Brilliant UV 737 Brilliant UV 805 Functional Grade NovaFluor Red 700 NovaFluor Red 755 NovaFluor Yellow 690 NovaFluor Yellow 700 PE PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 780 Unconjugated
Excitation/Emission Max	569/780 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2573509

Product Specific Information

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Non-synergy of PD-1 blockade with T-cell therapy in solid tumors.

Journal for immunotherapy of cancer

Davies JS,Karimipour F,Zhang L,Nagarsheth N,Norberg S,Serna C,Strauss J,Chiou S,Gulley JL,Hinrichs CS

25-5982-82 was used in Flow cytometry/Cell sorting to support the concept that PD-1 blockade acts primarily through endogenous rather than transferred T cells to mediate a non-synergistic antitumor effect in solid tumor cell therapy.

Fri Jul 01 00:00:00 EDT 2022

Heterogeneity of tyrosine-based melanin anabolism regulates pulmonary and cerebral organotropic colonization microenvironment of melanoma cells.

Theranostics

Wang X,Chen Y,Lan B,Wang Y,Lin W,Jiang X,Ye J,Shang B,Feng C,Liu J,Zhai J,Xu M,Li Q,Lin L,Hu M,Zheng F,Chen L,Shao C,Wang Y,Shi Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2022

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

25-5982-82 was used in Flow Cytometry to show that the deubiquitinase USP12 is commonly downregulated in the KrasG12D-driven mouse lung tumour and human non-small cell lung cancer owing to the activation of AKT-mTOR signalling.

Wed Aug 11 00:00:00 EDT 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

Wed Aug 11 00:00:00 EDT 2021

Collagenase-Expressing Salmonella Targets Major Collagens in Pancreatic Cancer Leading to Reductions in Immunosuppressive Subsets and Tumor Growth.

Cancers

Ebelt ND,Zamloot V,Zuniga E,Passi KB,Sobocinski LJ,Young CA,Blazar BR,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Oncolytic herpes simplex virus HF10 (canerpaturev) promotes accumulation of CD8⁺ PD-1^- tumor-infiltrating T cells in PD-L1-enriched tumor microenvironment.

International journal of cancer

Eissa IR,Mukoyama N,Abdelmoneim M,Naoe Y,Matsumura S,Bustos-Villalobos I,Ichinose T,Miyajima N,Morimoto D,Tanaka M,Fujimoto Y,Sone M,Kodera Y,Kasuya H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity.

Cell reports

Wang Y,Song M,Liu M,Zhang G,Zhang X,Li MO,Ma X,Zhang JJ,Huang XY

25-5982-82 was used in Flow Cytometry to investigate the effects of fascin blockade in anti-tumor immune responses in syngeneic mouse models of various cancers.

Tue Apr 06 00:00:00 EDT 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

Radiation-induced abscopal effect and its enhancement by programmed cell death 1 blockade in the hepatocellular carcinoma: A murine model study.

Clinical and molecular hepatology

Yoo GS,Ahn WG,Kim SY,Kang W,Choi C,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation.

Journal for immunotherapy of cancer

Wu B,Chiang HC,Sun X,Yuan B,Mitra P,Hu Y,Curiel TJ,Li R

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Downregulation of PD-L1 via amide analogues of brefelamide: Alternatives to antibody-based cancer immunotherapy.

Experimental and therapeutic medicine

Zhang J,Yamada O,Kida S,Murase S,Hattori T,Oshima Y,Kikuchi H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

GAPDH Overexpression in the T Cell Lineage Promotes Angioimmunoblastic T Cell Lymphoma through an NF-κB-Dependent Mechanism.

Cancer cell

Mondragón L,Mhaidly R,De Donatis GM,Tosolini M,Dao P,Martin AR,Pons C,Chiche J,Jacquin M,Imbert V,Proïcs E,Boyer L,Doye A,Luciano F,Neels JG,Coutant F,Fabien N,Sormani L,Rubio-Patiño C,Bossowski JP,Muller F,Marchetti S,Villa E,Peyron JF,Gaulard P,Lemonnier F,Asnafi V,Genestier L,Benhida R,Fournié JJ,Passeron T,Ricci JE,Verhoeyen E

25-5982-82 was used in Flow Cytometry to suggest the therapeutic potential of targeting NF-κB signaling in AITL and provide a model for future AITL therapeutic investigations.

Mon Sep 16 00:00:00 EDT 2019

Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.

Molecular oncology

Zhou Z,Chen H,Xie R,Wang H,Li S,Xu Q,Xu N,Cheng Q,Qian Y,Huang R,Shao Z,Xiang M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

MERTK inhibition alters the PD-1 axis and promotes anti-leukemia immunity.

JCI insight

Lee-Sherick AB,Jacobsen KM,Henry CJ,Huey MG,Parker RE,Page LS,Hill AA,Wang X,Frye SV,Earp HS,Jordan CT,DeRyckere D,Graham DK

25-5982 was used in Flow cytometry/Cell sorting to demonstrate both the direct therapeutic effects of MERTK inhibition on leukaemia cells and the induction of anti-leukaemia immunity via suppression of the coinhibitory PD-1 axis.

Fri Nov 02 00:00:00 EDT 2018

PD-1/PD-L1 regulates Treg differentiation in pregnancy-induced hypertension.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Jiang L,Tang C,Gong Y,Liu Y,Rao J,Chen S,Qu W,Wu D,Lei L,Chen L

25-5982 was used in Flow cytometry to study the relationship between PD-1/PD-L1 and regulatory T cell differentiation in pregnancy-induced hypertension.

Mon Jun 25 00:00:00 EDT 2018

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.

Immunity

Coelho MA,de Carné Trécesson S,Rana S,Zecchin D,Moore C,Molina-Arcas M,East P,Spencer-Dene B,Nye E,Barnouin K,Snijders AP,Lai WS,Blackshear PJ,Downward J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Tue Dec 19 00:00:00 EST 2017

Loss of MAPK-activated protein kinase 2 enables potent dendritic cell-driven anti-tumour T cell response.

Scientific reports

Soukup K,Halfmann A,Dillinger B,Poyer F,Martin K,Blauensteiner B,Kauer M,Kuttke M,Schabbauer G,Dohnal AM

25-5982 was used in Flow cytometry/Cell sorting to suggest that MAPK-activated protein kinase 2 deletion leads to an expansion of stimulatory CD103+ dendritic cells, mounting a potent CD8+ T cell anti-tumour response in mice.

Mon Sep 18 00:00:00 EDT 2017

Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition.

Oncotarget

Peer S,Baier G,Gruber T

25-5982 was used in Flow cytometry/Cell sorting to show that Cblb-/- T cells are less susceptible to PD-L1-mediated suppression of T cell proliferation and IFNγ secretion.

Tue Jun 27 00:00:00 EDT 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance.

The Journal of pharmacology and experimental therapeutics

Yang W,Yamada M,Tamura Y,Chang K,Mao J,Zou L,Feng Y,Kida K,Scherrer-Crosbie M,Chao W,Ichinose F,Yu YM,Fischman AJ,Tompkins RG,Yao S,Kaneki M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Ex vivo expanded hematopoietic stem cells overcome the MHC barrier in allogeneic transplantation.

Cell stem cell

Zheng J,Umikawa M,Zhang S,Huynh H,Silvany R,Chen BP,Chen L,Zhang CC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 25-5982-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2011

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

Fri Aug 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1

UniProt ID: (Mouse) Q9EP73

Entrez Gene ID: (Mouse) 60533

Molecular Function: immunoglobulin receptor superfamily

Function(s)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production positive regulation of T cell proliferation negative regulation of T cell proliferation negative regulation of activated T cell proliferation toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

Process(es)

protein binding

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE-Cyanine7, eBioscience™

Product Details

25-5982-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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Type

Clone

Conjugate

Excitation/Emission Max

Form

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Purification

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Contains

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RRID

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