Invitrogen

CD275 (B7-H2) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (42) CD275 (B7-H2) antibodies

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Datasheet

Protocols

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CD275 (B7-H2) Antibody in Western Blot (WB) — Western Blot was performed using Anti-CD275 (B7-H2) Polyclonal Antibody (Product # PA5-47177) and 50-80 kDa bands corresponding to glycosylated ICOS ligand was observed across cell lines tested except in U-87 MG and Jurkat, which are reported low expressing for the protein, and was upregulated upon TNF-alpha treatment in HUVEC cells. Whole cell extracts (40 µg lysate) of T98G (Lane 1), U-87 MG (Lane 2), Daudi (Lane 3), Jurkat (Lane 4), U-937 (Lane 5), untreated HUVEC (Lane 6) and TNF alpha treated HUVEC (100 units/mL for 24 hr) (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.15 µg/mL dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CD275 (B7-H2) Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of U937 human histiocytic lymphoma cell line was stained with Goat Anti-human B7-H2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47177, filled histogram) or isotype control antibodyopen histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.

CD275 (B7-H2) Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of ICOS ligand was observed in T98G as compared to U-87 MG and in Daudi and U-937 as compared to Jurkat using Anti-CD275 (B7-H2) Polyclonal Antibody (Product # PA5-47177) in Western Blot. {RE}

Product Details

PA5-47177

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.15 µg/mL	-
Flow Cytometry (Flow)	2.5 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Mouse myeloma cell line NS0-derived recombinant human B7-H2 Asp19-Ser258 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2610087

Product Specific Information

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse B7-H2 and recombinant human B7-H1 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B7 homolog 2; B7 homologue 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; inducible T-cell co-stimulator ligand; transmembrane protein B7-H2 ICOS ligand

Gene Aliases: B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; ICOSLG; KIAA0653; LICOS

UniProt ID: (Human) O75144

Entrez Gene ID: (Human) 23308

Molecular Function: immunoglobulin receptor superfamily