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Invitrogen
CD275 (B7-H2) Polyclonal Antibody
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FIGURE: 1 / 5
CD275 (B7-H2) Antibody (PA5-47177) in WB
Western Blot was performed using Anti-CD275 (B7-H2) Polyclonal Antibody (Product # PA5-47177) and 50-80 kDa bands corresponding to glycosylated ICOS ligand was observed across cell lines tested except in U-87 MG and Jurkat, which are reported low expressing for the protein, and was upregulated upon TNF-alpha treatment in HUVEC cells. Whole cell extracts (40 µg lysate) of T98G (Lane 1), U-87 MG (Lane 2), Daudi (Lane 3), Jurkat (Lane 4), U-937 (Lane 5), untreated HUVEC (Lane 6) and TNF alpha treated HUVEC (100 units/mL for 24 hr) (Lane 7) we... View More
Product Details
PA5-47177
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7-H2 Asp19-Ser258
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610087
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse B7-H2 and recombinant human B7-H1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 2; B7 homologue 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; inducible T-cell co-stimulator ligand; transmembrane protein B7-H2 ICOS ligand
Gene Aliases: B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; ICOSLG; KIAA0653; LICOS
UniProt ID: (Human) O75144
Entrez Gene ID: (Human) 23308
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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