Invitrogen

CD278 (ICOS) Monoclonal Antibody (ISA-3), eBioscience™

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CD278 (ICOS) Antibody in Flow Cytometry (Flow) — Staining of CD3 + CD28 stimulated human PBMC with Anti-Human CD278 (ICOS) FITC (left), and Anti-Human CD278 (ICOS) PE (right).Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

CD278 (ICOS) Antibody in T-Cell Activation (TCA) — Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in T-Cell Activation

Product Details

14-9948-82

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Flow Cytometry (Flow)	0.5 µg/test	View 11 publications 11 publications
Immunoprecipitation (IP)	Assay-Dependent	-
Functional Assay (FN)	Assay-Dependent	View 1 publication 1 publication
T-Cell Activation (TCA)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	ISA-3
Conjugate	Unconjugated Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 661 eFluor 450 FITC Functional Grade NovaFluor Red 660 NovaFluor Red 710 NovaFluor Yellow 700 NovaFluor Yellow 730 PE PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 780
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_468635

Product Specific Information

Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949-82), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948-82 or 12-9948-42 rather than 11-9948-42.

Applications Reported: The ISA-3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vitro functional assays. (Please use Functional Grade purified ISA-3, cat. 16-9948, in functional assays.).

Applications Tested: The ISA-3 antibody has been tested by flow cytometric analysis of unstimulated and PHA-activated (2 days) human blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Frontiers in immunology

Khanam A,Ayithan N,Tang L,Poonia B,Kottilil S

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Wed Jun 16 00:00:00 EDT 2021

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

The adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes.

The Journal of experimental medicine

Hill DL,Pierson W,Bolland DJ,Mkindi C,Carr EJ,Wang J,Houard S,Wingett SW,Audran R,Wallin EF,Jongo SA,Kamaka K,Zand M,Spertini F,Daubenberger C,Corcoran AE,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Mon Aug 05 00:00:00 EDT 2019

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

ICOS⁺ Foxp3⁺ TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.

International journal of cancer

Nagase H,Takeoka T,Urakawa S,Morimoto-Okazawa A,Kawashima A,Iwahori K,Takiguchi S,Nishikawa H,Sato E,Sakaguchi S,Mori M,Doki Y,Wada H

14-9948 was used in Flow cytometry/Cell sorting to demonstrate the potential of TCR-inducible costimulatory receptor (ICOS) as a promising target for direct Treg-targeting therapeutic agents for gastric cancer.

Wed Feb 01 00:00:00 EST 2017

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Polyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS.

Oncoimmunology

Franzese O,Palermo B,Di Donna C,Sperduti I,Ferraresi V,Stabile H,Gismondi A,Santoni A,Nisticò P

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in T-Cell Activation

Sun May 01 00:00:00 EDT 2016

PD-1 marks dysfunctional regulatory T cells in malignant gliomas.

JCI insight

Lowther DE,Goods BA,Lucca LE,Lerner BA,Raddassi K,van Dijk D,Hernandez AL,Duan X,Gunel M,Coric V,Krishnaswamy S,Love JC,Hafler DA

14-9948 was used in Flow cytometry/Cell sorting to study how high PD-1 expression on human Tregs identifies dysfunctional, exhausted Tregs secreting IFN-γ that exist in healthy individuals and are enriched in tumor infiltrates.

Thu Apr 21 00:00:00 EDT 2016

Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.

Oncoimmunology

Pedroza-Gonzalez A,Zhou G,Vargas-Mendez E,Boor PP,Mancham S,Verhoef C,Polak WG,Grünhagen D,Pan Q,Janssen H,Garcia-Romo GS,Biermann K,Tjwa ET,IJzermans JN,Kwekkeboom J,Sprengers D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9948-82) in T-Cell Activation

Mon Jun 01 00:00:00 EDT 2015

Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line.

Immunology and cell biology

Otani T,Tsuji-Takayama K,Okochi A,Yamamoto M,Takeuchi M,Yamasaki F,Nakamura S,Kibata M

14-9948 was used in Flow cytometry/Cell sorting to reveal that anti-mouse B7-1 Ab could effectively block IL-10 production, indicating a key role of the B7-1/CD28 pathway.

Tue Feb 01 00:00:00 EST 2011

Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Quiroga MF,Pasquinelli V,Martínez GJ,Jurado JO,Zorrilla LC,Musella RM,Abbate E,Sieling PA,García VE

Mon May 15 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CD278; inducible costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator

Gene Aliases: AILIM; CD278; CVID1; ICOS

UniProt ID: (Human) Q9Y6W8

Entrez Gene ID: (Human) 29851

Function(s)

T cell tolerance induction immune response single organismal cell-cell adhesion T cell costimulation

CD278 (ICOS) Monoclonal Antibody (ISA-3), eBioscience™

Product Details

14-9948-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (FN)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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