Invitrogen

CD278 (ICOS) Monoclonal Antibody (ISA-3), Alexa Fluor™ 700, eBioscience™

Product Image Gallery

CD278 (ICOS) Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were unstimulated (left) or stimulated for 72 hours with CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85) and (Product # 16-0289-85) (right). Cells were then stained with CD25 Monoclonal Antibody, PE-Cyanine7 (Product # 25-0259-42) and CD278 (ICOS) Monoclonal Antibody, Alexa Fluor 700. Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

CD278 (ICOS) Antibody in Functional assay (FN) — Figure 6. ICOS is upregulated in Melan-A-specific CD8 + T cells isolated after combined therapy and is involved in chemoimmunotherapy-induced AKT activation. (A) Top panel shows representative staining with anti-ICOS (open histogram) or isotype (filled histogram) mAbs, in two T-cell clones isolated from Arm1 (Pt08.90) and Arm2 (Pt15.26) patients after the treatments (POST). Bottom panel shows pooled data of ICOS expression in Melan-A-specific T-cell clones (n = 15 from two patients of Arm1, n = 18 from three patients of Arm2), isolated before (PRE) and after therapy (POST). Cells were stained after 48 h stimulation with plate-bound anti-CD3 mAb (2 ug/mL) plus rIL-2 and shown in both scatter-plot graph and box-and-whisker diagram, with 5-95 percentile. Each dot represents the mean value out of three-five independent experiments. The mean +- SEM of each group is shown. (B) Correlation between p-AKT and ICOS expression. T-cell clones isolated after both treatments were grouped according to pSerAKT expression levels and the percentage of ICOS + cells (bottom panel) and shows higher expression of ICOS in AKT high T cells. Top panel, percentage of clones with ICOS levels > 30% cut-off in p-AKT neg/low versus p-AKT high groups. p value was calculated with one-tail Fisher''s exact test. * / p <= 0.05, ** / p <= 0.01, *** / p <= 0.001, Mann-Whitney two-sample test (*) and two-tail Student''s test ( ), respectively. (C) Left panel: effect of ICOS blockade on AKT activation (p-Ser473-AK

Product Details

56-9948-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.5 µg)/test	View 10 publications 10 publications
Functional Assay (FN)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	ISA-3
Conjugate	Alexa Fluor™ 700 APC APC-eFluor 780 Biotin Brilliant UV 661 eFluor 450 FITC Functional Grade NovaFluor Red 660 NovaFluor Red 710 NovaFluor Yellow 700 NovaFluor Yellow 730 PE PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 780 Unconjugated
Excitation/Emission Max	696/719 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2848478

Product Specific Information

Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949-82), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948-82 or 12-9948-42 rather than 11-9948-42.

Applications Reported: The ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ISA-3 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser

Target Information

CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Frontiers in immunology

Khanam A,Ayithan N,Tang L,Poonia B,Kottilil S

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Wed Jun 16 00:00:00 EDT 2021

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

The adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes.

The Journal of experimental medicine

Hill DL,Pierson W,Bolland DJ,Mkindi C,Carr EJ,Wang J,Houard S,Wingett SW,Audran R,Wallin EF,Jongo SA,Kamaka K,Zand M,Spertini F,Daubenberger C,Corcoran AE,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Mon Aug 05 00:00:00 EDT 2019

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2018

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Polyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS.

Oncoimmunology

Franzese O,Palermo B,Di Donna C,Sperduti I,Ferraresi V,Stabile H,Gismondi A,Santoni A,Nisticò P

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Functional assay

Sun May 01 00:00:00 EDT 2016

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.

Oncoimmunology

Pedroza-Gonzalez A,Zhou G,Vargas-Mendez E,Boor PP,Mancham S,Verhoef C,Polak WG,Grünhagen D,Pan Q,Janssen H,Garcia-Romo GS,Biermann K,Tjwa ET,IJzermans JN,Kwekkeboom J,Sprengers D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 56-9948-42) in Functional assay

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CD278; inducible costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator

Gene Aliases: AILIM; CD278; CVID1; ICOS

UniProt ID: (Human) Q9Y6W8

Entrez Gene ID: (Human) 29851

Function(s)

T cell tolerance induction immune response single organismal cell-cell adhesion T cell costimulation

CD278 (ICOS) Monoclonal Antibody (ISA-3), Alexa Fluor™ 700, eBioscience™

Product Details

56-9948-42

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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