Invitrogen

CD279 (PD-1) Monoclonal Antibody (J43), PerCP-eFluor™ 710, eBioscience™

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CD279 (PD-1) Antibody in Flow Cytometry (Flow) — Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.06 µg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (blue histogram) or 0.06 µg of Anti-Mouse CD279 (PD-1) PerCP-eFluor® 710 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

Product Details

46-9985-82

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.125 µg/test	View 30 publications 30 publications
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Armenian hamster / IgG
Recommended Isotype Control	Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-eFluor™ 710, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	J43
Conjugate	PerCP-eFluor™ 710 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 FITC Functional Grade PE PE-Cyanine7 PE-eFluor 610 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11150055

Product Specific Information

Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Journal for immunotherapy of cancer

Hu J,He J,Wang Y,Zhao Y,Fang K,Dong Y,Chen Y,Zhang Y,Zhang C,Wang H,Tan J,Wang J,Zi R,Liu C,Liang H,Guo Y,Ou J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury.

The Journal of clinical investigation

Sato Y,Oguchi A,Fukushima Y,Masuda K,Toriu N,Taniguchi K,Yoshikawa T,Cui X,Kondo M,Hosoi T,Komidori S,Shimizu Y,Fujita H,Jiang L,Kong Y,Yamanashi T,Seita J,Yamamoto T,Toyokuni S,Hamazaki Y,Hattori M,Yoshikai Y,Boor P,Floege J,Kawamoto H,Murakawa Y,Minato N,Yanagita M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Dec 02 00:00:00 EST 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma.

Frontiers in immunology

Ebelt ND,Zuniga E,Johnson BL,Diamond DJ,Manuel ER

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Nature immunology

Gu M,Zhou X,Sohn JH,Zhu L,Jie Z,Yang JY,Zheng X,Xie X,Yang J,Shi Y,Brightbill HD,Kim JB,Wang J,Cheng X,Sun SC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

Therapeutic efficacy of combined vaccination against tumor pericyte-associated antigens DLK1 and DLK2 in mice.

Oncoimmunology

Fabian KP,Chi-Sabins N,Taylor JL,Fecek R,Weinstein A,Storkus WJ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity.

Cell reports

Zhou X,Zhu X,Li C,Li Y,Ye Z,Shapiro VS,Copland JA,Hitosugi T,Bernlohr DA,Sun J,Zeng H

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 05 00:00:00 EST 2021

Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection.

The Journal of experimental medicine

Fumagalli V,Di Lucia P,Venzin V,Bono EB,Jordan R,Frey CR,Delaney W,Chisari FV,Guidotti LG,Iannacone M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2020

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses.

Antioxidants (Basel, Switzerland)

Lee HJ,Park JS,Yoo HJ,Lee HM,Lee BC,Kim JH

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2020

IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression.

Cell

Sadik A,Somarribas Patterson LF,Öztürk S,Mohapatra SR,Panitz V,Secker PF,Pfänder P,Loth S,Salem H,Prentzell MT,Berdel B,Iskar M,Faessler E,Reuter F,Kirst I,Kalter V,Foerster KI,Jäger E,Guevara CR,Sobeh M,Hielscher T,Poschet G,Reinhardt A,Hassel JC,Zapatka M,Hahn U,von Deimling A,Hopf C,Schlichting R,Escher BI,Burhenne J,Haefeli WE,Ishaque N,Böhme A,Schäuble S,Thedieck K,Trump S,Seiffert M,Opitz CA

46-9985 was used in Flow cytometry/Cell sorting to show that IL4I1 blockade opens new avenues for cancer therapy.

Thu Sep 03 00:00:00 EDT 2020

Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells.

The Journal of clinical investigation

Khiew SH,Jain D,Chen J,Yang J,Yin D,Young JS,Dent A,Sciammas R,Alegre ML,Chong AS

46-9985 was used in Flow cytometry/Cell sorting to showed that alloreactive B cells were not deleted but rapidly lost their ability to differentiate into germinal center B cells and secrete donor-specific antibodies., by using a murine model of heart tolerance.

Wed Jul 01 00:00:00 EDT 2020

Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion.

Oncology letters

Shang K,Wang Z,Hu Y,Huang Y,Yuan K,Yu Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model.

Oncoimmunology

Ye X,Waite JC,Dhanik A,Gupta N,Zhong M,Adler C,Malahias E,Ni M,Wei Y,Gurer C,Zhang W,Macdonald LE,Murphy AJ,Sleeman MA,Skokos D

46-9985 was used in Flow cytometry/Cell sorting to reveal that endogenous retroviral antigens expressed by tumour cells may represent an important and underappreciated category of tumour antigens that could be readily targeted in the clinic.

Wed May 13 00:00:00 EDT 2020

Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross.

Cell reports

Martin MD,Sompallae R,Winborn CS,Harty JT,Badovinac VP

46-9985 was used in Flow cytometry/Cell sorting to advance the utility of the Collaborative Cross (CC) as a tool to analyze the immune response to viral infection.

Tue Apr 14 00:00:00 EDT 2020

CXCR5⁺PD-1⁺ follicular helper CD8 T cells control B cell tolerance.

Nature communications

Chen Y,Yu M,Zheng Y,Fu G,Xin G,Zhu W,Luo L,Burns R,Li QZ,Dent AL,Zhu N,Cui W,Malherbe L,Wen R,Wang D

46-9985 was used in Flow cytometry/Cell sorting to highlight the diversity of follicular T cell subsets that contribute to the breakdown of B cell tolerance.

Fri Sep 27 00:00:00 EDT 2019

Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma.

Cell reports

Burrack AL,Spartz EJ,Raynor JF,Wang I,Olson M,Stromnes IM

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2019

Repurposing the selective estrogen receptor modulator bazedoxifene to suppress gastrointestinal cancer growth.

EMBO molecular medicine

Thilakasiri P,Huynh J,Poh AR,Tan CW,Nero TL,Tran K,Parslow AC,Afshar-Sterle S,Baloyan D,Hannan NJ,Buchert M,Scott AM,Griffin MD,Hollande F,Parker MW,Putoczki TL,Ernst M,Chand AL

46-9985-82 was used in Flow Cytometry to establish the in vivo utility of bazedoxifene, a steroid analog clinically approved for the treatment of osteoporosis, to suppress gp130-dependent tumor growth of the gastrointestinal epithelium.

Mon Apr 01 00:00:00 EDT 2019

Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse.

Cell reports

Obino D,Fetler L,Soza A,Malbec O,Saez JJ,Labarca M,Oyanadel C,Del Valle Batalla F,Goles N,Chikina A,Lankar D,Segovia-Miranda F,Garcia C,Léger T,Gonzalez A,Espéli M,Lennon-Duménil AM,Yuseff MI

46-9985 was used in Flow cytometry/Cell sorting to examine the mechanism by which Galectin-8 favours the presentation of surface-tethered antigens.

Tue Dec 11 00:00:00 EST 2018

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Sat Jul 15 00:00:00 EDT 2017

Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition.

Oncotarget

Peer S,Baier G,Gruber T

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jun 27 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX