|Tested species reactivity||Mouse|
|Host / Isotype||Syrian hamster / IgG|
|Immunogen||Mouse T cell lymphoma EL-4 cells|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||6 ug/ml|
|Functional Assay (FN)||Assay Dependent|
|Immunocytochemistry (ICC)||1-4 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1-4 ug/ml|
|Immunoprecipitation (IP)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with the extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.