|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Syrian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.
Applications Reported: This 37.51 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells.||Nandi D,Gross JA,Allison JP||Journal of immunology (Baltimore, Md. : 1950) (152:3361)||1994|
|Not Applicable||Not Cited||Identification and distribution of the costimulatory receptor CD28 in the mouse.||Gross JA,Callas E,Allison JP||Journal of immunology (Baltimore, Md. : 1950) (149:380)||1992|
|Not Applicable||Not Cited||CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.||Harding FA,McArthur JG,Gross JA,Raulet DH,Allison JP||Nature (356:607)||1992|
|Not Applicable||Not Cited||The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression.||Gross JA,St John T,Allison JP||Journal of immunology (Baltimore, Md. : 1950) (144:3201)||1990|