Invitrogen

CD3 Monoclonal Antibody (UCHT1), Biotin, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

38 Published Figures

28 References

View all (460) CD3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD3 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human CD3 PE-Cy5 (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

CD3 Antibody in Immunocytochemistry (ICC/IF) — Figure 9 Representative high-magnification photomicrographs showing double immunofluorescence for FAAH, NAPE-PLD, CD38 and CD3 in order to characterize the immune cells in the mucosa infiltrate of UC patients. Nearly all FAAH immunofluorescent cells are plasma cell-specific CD38 (A-C). NAPE-PLD immunofluorescence was observed in both CD38+ plasma cells (D-F) and CD3+ T lymphocytes (G-I).

CD3 Antibody in Immunohistochemistry (IHC) — Figure 6 Representative high-magnification photomicrographs showing double immunofluorescence for NAAA, CD19, CD3 and CD14 in order to characterize the immune cells in the mucosa infiltrate of UC patients. NAAA immunofluorescence was observed in CD19+ B lymphocytes (A-C), CD3+ T lymphocytes (D-F) and CD14+ macrophages (G-I).

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis.

Product Details

13-0038-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Functional Assay (FN)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_466323

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The UCHT1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant gut-microbiota-immune-brain axis development in premature neonates with brain damage.

Cell host & microbe

Seki D,Mayer M,Hausmann B,Pjevac P,Giordano V,Goeral K,Unterasinger L,Klebermaß-Schrehof K,De Paepe K,Van de Wiele T,Spittler A,Kasprian G,Warth B,Berger A,Berry D,Wisgrill L

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Wed Oct 13 00:00:00 EDT 2021

Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4⁺ T Lymphocytes.

Frontiers in cellular and infection microbiology

Perot BP,García-Paredes V,Luka M,Ménager MM

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma.

Cell research

Chen YP,Yin JH,Li WF,Li HJ,Chen DP,Zhang CJ,Lv JW,Wang YQ,Li XM,Li JY,Zhang PP,Li YQ,He QM,Yang XJ,Lei Y,Tang LL,Zhou GQ,Mao YP,Wei C,Xiong KX,Zhang HB,Zhu SD,Hou Y,Sun Y,Dean M,Amit I,Wu K,Kuang DM,Li GB,Liu N,Ma J

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Skewed T cell responses to Epstein-Barr virus in long-term asymptomatic kidney transplant recipients.

PloS one

Nakid-Cordero C,Arzouk N,Gauthier N,Tarantino N,Larsen M,Choquet S,Burrel S,Autran B,Vieillard V,Guihot A

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Mon Mar 30 00:00:00 EDT 2020

Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity.

The Journal of clinical investigation

Randzavola LO,Strege K,Juzans M,Asano Y,Stinchcombe JC,Gawden-Bone CM,Seaman MN,Kuijpers TW,Griffiths GM

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Mon Dec 02 00:00:00 EST 2019

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

The Journal of clinical investigation

Maffucci P,Chavez J,Jurkiw TJ,O'Brien PJ,Abbott JK,Reynolds PR,Worth A,Notarangelo LD,Felgentreff K,Cortes P,Boisson B,Radigan L,Cobat A,Dinakar C,Ehlayel M,Ben-Omran T,Gelfand EW,Casanova JL,Cunningham-Rundles C

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Mon Dec 03 00:00:00 EST 2018

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36.

PloS one

Krupna-Gaylord MA,Liveris D,Love AC,Wormser GP,Schwartz I,Petzke MM

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2015

A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood.

Epigenetics

Wiencke JK,Bracci PM,Hsuang G,Zheng S,Hansen H,Wrensch MR,Rice T,Eliot M,Kelsey KT

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases.

Scientific reports

Mock U,Riecken K,Berdien B,Qasim W,Chan E,Cathomen T,Fehse B

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Thu Sep 18 00:00:00 EDT 2014

Trypan blue exclusion assay by flow cytometry.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Avelar-Freitas BA,Almeida VG,Pinto MC,Mourão FA,Massensini AR,Martins-Filho OA,Rocha-Vieira E,Brito-Melo GE

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Human mesenchymal stromal cells modulate T-cell responses through TNF-α-mediated activation of NF-κB.

European journal of immunology

Dorronsoro A,Ferrin I,Salcedo JM,Jakobsson E,Fernández-Rueda J,Lang V,Sepulveda P,Fechter K,Pennington D,Trigueros C

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2014

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Recombinant influenza virus carrying the respiratory syncytial virus (RSV) F85-93 CTL epitope reduces RSV replication in mice.

Journal of virology

De Baets S,Schepens B,Sedeyn K,Schotsaert M,Roose K,Bogaert P,Fiers W,Saelens X

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis.

International journal of nanomedicine

Cheng HY,Lai LJ,Ko FH

13-0038 was used in Magnetic cell separation to develop a rapid and sensitive method for detecting cancer cells occurring at low concentration.

Thu Jan 24 00:00:00 EST 2013

TCR activation kinetics and feedback regulation in primary human T cells.

Cell communication and signaling : CCS

Poltorak M,Arndt B,Kowtharapu BS,Reddycherla AV,Witte V,Lindquist JA,Schraven B,Simeoni L

13-0038 was used in Functional assays to analyse the signaling dynamics correlating with either unresponsiveness or proliferation induced upon TCR/CD28 ligation in primary human T cells, suggesting that alternative feedback regulation occurs during these conditions.

Mon Jan 14 00:00:00 EST 2013

Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids.

PloS one

Suárez J,Romero-Zerbo Y,Márquez L,Rivera P,Iglesias M,Bermúdez-Silva FJ,Andreu M,Rodríguez de Fonseca F

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Immunofluorescence

Thu Dec 13 00:00:00 EST 2012

Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids.

PloS one

Suárez J,Romero-Zerbo Y,Márquez L,Rivera P,Iglesias M,Bermúdez-Silva FJ,Andreu M,Rodríguez de Fonseca F

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Immunofluorescence

Thu Dec 13 00:00:00 EST 2012

Epigenetic biomarkers of T-cells in human glioma.

Epigenetics

Wiencke JK,Accomando WP,Zheng S,Patoka J,Dou X,Phillips JJ,Hsuang G,Christensen BC,Houseman EA,Koestler DC,Bracci P,Wiemels JL,Wrensch M,Nelson HH,Kelsey KT

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory.

The Journal of experimental medicine

Kaji T,Ishige A,Hikida M,Taka J,Hijikata A,Kubo M,Nagashima T,Takahashi Y,Kurosaki T,Okada M,Ohara O,Rajewsky K,Takemori T

13-0038 was used in Flow cytometry/Cell sorting to reveal distinct cellular pathways that select germline-encoded and somatically mutated antibodies into immunological memory.

Mon Oct 22 00:00:00 EDT 2012

Accumulation of natural killer T cells in progressive nonalcoholic fatty liver disease.

Hepatology (Baltimore, Md.)

Syn WK,Oo YH,Pereira TA,Karaca GF,Jung Y,Omenetti A,Witek RP,Choi SS,Guy CD,Fearing CM,Teaberry V,Pereira FE,Adams DH,Diehl AM

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Immunohistochemistry

Tue Jun 01 00:00:00 EDT 2010

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

PLoS medicine

Senechal B,Elain G,Jeziorski E,Grondin V,Patey-Mariaud de Serre N,Jaubert F,Beldjord K,Lellouch A,Glorion C,Zerah M,Mary P,Barkaoui M,Emile JF,Boccon-Gibod L,Josset P,Debré M,Fischer A,Donadieu J,Geissmann F

Published figure using CD3 monoclonal antibody (Product # 13-0038-82) in Immunohistochemistry

Wed Aug 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily