|Flow Cytometry (Flow)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Clone||M2AB, 1G9, BHPT-1|
|Immunogen||human thymocytes and human peripheral blood monocytes from a rheumatoid arthritis patient|
|Conjugate||PE , PE-Cyanine5|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1), PE-conjugated CD19 (clone 1G9, isotype IgG1) and Cy5-PE-conjugated CD45 (clone BHPT-1, isotype IgG1).
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. CD19 assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN.
Protein Aliases: AW495831; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B220; c polypeptide; CD19; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD45; CD45 antigen; CD45R; Differentiation antigen CD19; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; GP180; L-CA; LCA; Leukocyte common antigen; LY5; OKT3, delta chain; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor type; Receptor-type tyrosine-protein phosphatase C; T-cell antigen receptor complex; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen Leu-12; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T200; T200 glycoprotein; T200 leukocyte common antigen; T3E; TCRE
Gene Aliases: B220; B4; CD19; CD3-DELTA; CD3-GAMMA; CD3D; CD3E; CD3G; CD45; CD45R; CVID3; GP180; IMD17; IMD18; IMD19; L-CA; LCA; LY5; PTPRC; T200; T3D; T3E; T3G; TCRE