|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1, IgM|
|Immunogen||Purified rat LCA|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
MA5-16726 contains a monoclonal mixture of FITC-conjugated CD3 (clone #IF4, Mouse IgM) and RPE-conjugated CD45RA (clone #OX-33, Mouse IgG1). This antibody is a dual-color reagent recognizing rat CD3 and CD45RA cell surface antigens. Clone IF4 recognizes rat CD3, a cell surface antigen expressed by mature lymphocytes. Clone OX-33 recognizes rat CD45RA, a cell surface antigen expressed by B-lymphocytes.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation.
MA5-16726 was used in flow cytometry to discuss tolerance to ACI heart allografts using rats.
|Lan F,Hayamizu K,Strober S||Transplantation (69:649)||2000|
Comparison of chimeric acid and non-chimeric tolerance using posttransplant total lymphoid irradiation: cytokine expression and chronic rejection.
MA5-16726 was used in flow cytometry to study tolerance induction using heart allografts in Lewis rat.
|Hayamizu K,Lan F,Huie P,Sibley RK,Strober S||Transplantation (68:1036)||1999|
Monocyte-derived dendritic cell precursors facilitate tolerance to heart allografts after total lymphoid irradiation.
MA5-16726 was used in flow cytometry to discuss factors that promote tolerance in a rat ACI heart allografts model.
|Hayamizu K,Huie P,Sibley RK,Strober S||Transplantation (66:1285)||1998|
Donor blood monocytes but not T or B cells facilitate long-term allograft survival after total lymphoid irradiation.
MA5-16726 was used in flow cytometry to determine the subset(s) of cells in donor blood that promote long-term allograft survival in rats.
|Hayamizu K,Zeng D,Huie P,Garcia-Ojeda ME,Bloch DA,Fong L,Engleman EG,Sibley RK,Strober S||Transplantation (66:585)||1998|