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|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1, IgM|
|Immunogen||Purified rat LCA|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-16726 contains a monoclonal mixture of FITC-conjugated CD3 (clone #IF4, Mouse IgM) and RPE-conjugated CD45RA (clone #OX-33, Mouse IgG1). This antibody is a dual-color reagent recognizing rat CD3 and CD45RA cell surface antigens. Clone IF4 recognizes rat CD3, a cell surface antigen expressed by mature lymphocytes. Clone OX-33 recognizes rat CD45RA, a cell surface antigen expressed by B-lymphocytes.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
B220; c polypeptide; CD3 antigen delta polypeptide; CD3 antigen, gamma polypeptide; CD3 epsilon; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, gamma polypeptide; CD3-epsilon; CD3e; CD3e antigen; CD45; CD45 antigen; CD45R; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; GP180; L-CA; LCA; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; LY5; protein tyrosine phosphatase; Protein tyrosine phosphatase, receptor-type, c polypeptide; PTPRC; receptor type; receptor-type tyrosine-protein phosphatase C; T-cell antigen receptor complex; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 gamma chain; T200 glycoprotein; T200 leukocyte common antigen; T3E; TCRE
Cd3d; Cd3g; CD45; L-CA; Lca; Ptprc; RT7; T200; T3d