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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FLK1 (VEGF Receptor 2) Antibodies

          Invitrogen

          CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™

          Advanced Verification

          This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

          48 Published Figures
          34 References
          View all (64) FLK1 (VEGF Receptor 2) antibodies
          Datasheet
          Protocols
          Tech Support

          Cite CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (1)
          • Published Figures (48)
          • Advanced Verification (1)
          CD309 (FLK1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 50

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          CD309 (FLK1) Antibody (14-5821-81) in Flow

          Surface staining of bEnd-3 cell line with Anti-Mouse CD309 (FLK1) PE.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          14-5821-81

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-Dependent
          -

          Immunohistochemistry (IHC)

          Assay-Dependent
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 2 publications 2 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 20 publications 20 publications

          Peptide Array (Array)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Dog, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          Avas12a1

          Conjugate

          Unconjugated
          • Alexa Fluor 700 
          • APC 
          • Biotin 
          • PE 
          • Super Bright 436
          • Super Bright 600

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Storage conditions

          4° C

          RRID

          AB_467622

          Product Specific Information

          Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

          Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining.

          Applications Tested: This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

          View more View less

          Gene Aliases: Flk-1; Flk1; Kdr

          View more View less

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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