Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

40 Published Figures

33 References

View all (64) FLK1 (VEGF Receptor 2) antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD309 (FLK1) Antibody in Flow Cytometry (Flow) — Surface staining of bEnd-3 cell line with Anti-Mouse CD309 (FLK1) PE.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

CD309 (FLK1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Immunocytochemistry and Flow Cytometry

CD309 (FLK1) Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Immunocytochemistry

CD309 (FLK1) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Immunohistochemistry (Paraffin)

CD309 (FLK1) Antibody in Immunohistochemistry (IHC) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Immunohistochemistry

CD309 (FLK1) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Immunohistochemistry and Flow Cytometry

CD309 (FLK1) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Immunohistochemistry (PFA fixed)

CD309 (FLK1) Antibody in Flow Cytometry, Peptide array (Flow, ARRAY) — Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-81) in Flow Cytometry and Peptide array

CD309 (FLK1) Antibody — Figure 6 Endothelial cell-macrophage interactions via VEGF and placental growth factor (PlGF). ( A ) Detection of retinal hypoxia and vessel perfusion at P8 and 4 weeks by i.p. pimonidazole and transcardiac Tomato lectin injections, respectively, along with IHC for CD31. Note the hypoxia in the areas of hypoperfused vessels in the APB-treated retinas. ( B ) Quantitative reverse transcription PCR (qRT-PCR) of P8 ( n = 8) and 8-week ( n = 6) retinas. ( C ) ISH for Vegfa (white) and IHC for type IV collagen (Col IV) and Iba1 in P8 retinas. ( D ) IHC for CD31 and VEGFR2 (white) in retinas from P8 and 4-week Vegfr2-BAC-GFP mice. Note the endothelial VEGFR2 upregulation in APB5-treated retinas, in contrast to the constitutive VEGFR2 expression in neurons and Muller glia. ( E ) ISH for Pgf (white) and IHC for Col IV and Iba1 in P8 retinas. ( F ) IHC for Iba1 in retinas from P8 and 4-wk Vegfr1-BAC-DsRed mice. ( G ) Flow cytometry in P8 retinas. CD45 hi CD11b + and CD45 lo CD11b + cells were gated as shown in Figure 5A . The graph shows fold changes of the number of CD45 hi CD11b + VEGFR1 + and CD45 lo CD11b + VEGFR1 + cells per retina ( n = 5). ( H ) Representative retina cups (upper) and IHC for CD31 and Iba1 (lower) at P11 in Flt1-TK mice. The APB5-treated retinas were graded according to Supplemental Figure 1C . The graphs show the number of Iba1 + cells and the vessel density per area (control, n = 12; APB5, n = 20). ( I ) The trajectory of GFP + cells in ex vivo retina imaging f

Product Details

14-5821-81

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

Assay-Dependent

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 20 publications 20 publications

Peptide Array (Array)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Dog, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Avas12a1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467622

Product Specific Information

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.

Nature communications

Chagraoui H,Kristiansen MS,Ruiz JP,Serra-Barros A,Richter J,Hall-Ponselé E,Gray N,Waithe D,Clark K,Hublitz P,Repapi E,Otto G,Sopp P,Taylor S,Thongjuea S,Vyas P,Porcher C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis.

JCI insight

Murray LA,Habiel DM,Hohmann M,Camelo A,Shang H,Zhou Y,Coelho AL,Peng X,Gulati M,Crestani B,Sleeman MA,Mustelin T,Moore MW,Ryu C,Osafo-Addo AD,Elias JA,Lee CG,Hu B,Herazo-Maya JD,Knight DA,Hogaboam CM,Herzog EL

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Sustained inflammation after pericyte depletion induces irreversible blood-retina barrier breakdown.

JCI insight

Ogura S,Kurata K,Hattori Y,Takase H,Ishiguro-Oonuma T,Hwang Y,Ahn S,Park I,Ikeda W,Kusuhara S,Fukushima Y,Nara H,Sakai H,Fujiwara T,Matsushita J,Ema M,Hirashima M,Minami T,Shibuya M,Takakura N,Kim P,Miyata T,Ogura Y,Uemura A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Thu Feb 09 00:00:00 EST 2017

Endothelial cell-anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice.

Molecular carcinogenesis

Wang J,Xiao J,Wen D,Wu X,Mao Z,Zhang J,Ma D

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry (Paraffin)

Sun May 01 00:00:00 EDT 2016

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Jul 14 00:00:00 EDT 2015

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

14-5821 was used in Immunohistochemistry to explore a molecular cascade that is potentially an early step in the programming of ESC differentiation.

Sat Nov 01 00:00:00 EDT 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

14-5821 was used in Immunohistochemistry to explore a molecular cascade that is potentially an early step in the programming of ESC differentiation.

Sat Nov 01 00:00:00 EDT 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

14-5821 was used in Immunohistochemistry to explore a molecular cascade that is potentially an early step in the programming of ESC differentiation.

Sat Nov 01 00:00:00 EDT 2014

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

Nature

Ubil E,Duan J,Pillai IC,Rosa-Garrido M,Wu Y,Bargiacchi F,Lu Y,Stanbouly S,Huang J,Rojas M,Vondriska TM,Stefani E,Deb A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Oct 30 00:00:00 EDT 2014

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Developmental biology

Shi X,Richard J,Zirbes KM,Gong W,Lin G,Kyba M,Thomson JA,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu May 15 00:00:00 EDT 2014

Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.

Stem cells (Dayton, Ohio)

Zhang H,Nieves JL,Fraser ST,Isern J,Douvaras P,Papatsenko D,D'Souza SL,Lemischka IR,Dyer MA,Baron MH

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Nkx2-5 mediates differential cardiac differentiation through interaction with Hoxa10.

Stem cells and development

Behrens AN,Iacovino M,Lohr JL,Ren Y,Zierold C,Harvey RP,Kyba M,Garry DJ,Martin CM

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Etv2 rescues Flk1 mutant embryoid bodies.

Genesis (New York, N.Y. : 2000)

Rasmussen TL,Martin CM,Walter CA,Shi X,Perlingeiro R,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.

PloS one

Sountoulidis A,Stavropoulos A,Giaglis S,Apostolou E,Monteiro R,Chuva de Sousa Lopes SM,Chen H,Stripp BR,Mummery C,Andreakos E,Sideras P

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Wed May 15 00:00:00 EDT 2013

Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.

PloS one

Sountoulidis A,Stavropoulos A,Giaglis S,Apostolou E,Monteiro R,Chuva de Sousa Lopes SM,Chen H,Stripp BR,Mummery C,Andreakos E,Sideras P

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Wed May 15 00:00:00 EDT 2013

VEGF/Flk1 signaling cascade transactivates Etv2 gene expression.

PloS one

Rasmussen TL,Shi X,Wallis A,Kweon J,Zirbes KM,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Mon May 13 00:00:00 EDT 2013

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Tumor-expressed inducible nitric oxide synthase controls induction of functional myeloid-derived suppressor cells through modulation of vascular endothelial growth factor release.

Journal of immunology (Baltimore, Md. : 1950)

Jayaraman P,Parikh F,Lopez-Rivera E,Hailemichael Y,Clark A,Ma G,Cannan D,Ramacher M,Kato M,Overwijk WW,Chen SH,Umansky VY,Sikora AG

14-5821 was used in Flow cytometry/Cell sorting to suggest a critical role for tumor-expressed inducible NO synthase in recruitment and induction of functional myeloid-derived suppressor cells.

Fri Jun 01 00:00:00 EDT 2012

Whole-mount confocal microscopy for vascular branching morphogenesis.

Methods in molecular biology (Clifton, N.J.)

Mukouyama YS,James J,Nam J,Uchida Y

14-5821 was used in Immunohistochemistry-wholemount to describe a protocol to stain intact blood vessels with vascular-specific antibodies and fluorescent secondary antibodies.

Thu Apr 26 00:00:00 EDT 2012

Eomesodermin induces Mesp1 expression and cardiac differentiation from embryonic stem cells in the absence of Activin.

EMBO reports

van den Ameele J,Tiberi L,Bondue A,Paulissen C,Herpoel A,Iacovino M,Kyba M,Blanpain C,Vanderhaeghen P

14-5821 was used in Flow cytometry/Cell sorting to investigate the range of cell fates controlled by Eomes and its mechanisms of action.

Sun Apr 01 00:00:00 EDT 2012

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2011

Recapitulation of the embryonic cardiovascular progenitor cell niche.

Biomaterials

Schenke-Layland K,Nsair A,Van Handel B,Angelis E,Gluck JM,Votteler M,Goldhaber JI,Mikkola HK,Kahn M,Maclellan WR

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2011

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Wed Sep 01 00:00:00 EDT 2010

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Wed Sep 01 00:00:00 EDT 2010

The effects of VEGF-R1 and VEGF-R2 ligands on angiogenic responses and left ventricular function in mice.

Cardiovascular research

Huusko J,Merentie M,Dijkstra MH,Ryhänen MM,Karvinen H,Rissanen TT,Vanwildemeersch M,Hedman M,Lipponen J,Heinonen SE,Eriksson U,Shibuya M,Ylä-Herttuala S

14-5821 was used in Immunohistochemistry on paraffin embedded tissues to analyse the angiogenic properties of ligands for different VEGF receptors in mouse myocardium.

Thu Apr 01 00:00:00 EDT 2010

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

14-5821 was used in Immunohistochemistry to investigate the mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells in mouse liver development.

Sun Mar 01 00:00:00 EST 2009

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

14-5821 was used in Immunohistochemistry to investigate the mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells in mouse liver development.

Sun Mar 01 00:00:00 EST 2009

Endogenous matrix metalloproteinase (MMP)-3 and MMP-9 promote the differentiation and migration of adult neural progenitor cells in response to chemokines.

Stem cells (Dayton, Ohio)

Barkho BZ,Munoz AE,Li X,Li L,Cunningham LA,Zhao X

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunocytochemistry

Mon Dec 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

Gene Aliases: 6130401C07; Flk-1; Flk1; Kdr; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2

UniProt ID: (Mouse) P35918

Entrez Gene ID: (Mouse) 16542

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding vascular endothelial growth factor binding

CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™

Product Details

14-5821-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Peptide Array (Array)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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