Flow cytometry analysis of CD31 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA3105) at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||Mouse PECAM-1 (CD31)|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3105 targets CD31 in FACS, and Neu applications and shows reactivity with mouse and Human samples.
The MA3105 immunogen is mouse PECAM-1 (CD31).
MA3105 detects CD31 which has a predicted molecular weight of approximately 79 kDa.
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo.
MA3105 was used in immunohistochemistry to study the role of RhoA in the mechanism by which afadin modulates murine embryo lymphangiogenesis
|Majima T,Takeuchi K,Sano K,Hirashima M,Zankov DP,Tanaka-Okamoto M,Ishizaki H,Miyoshi J,Ogita H||PloS one (8:null)||2016|
NG2 proteoglycan-dependent recruitment of tumor macrophages promotes pericyte-endothelial cell interactions required for brain tumor vascularization.
MA3105 was used in immunohistochemistry to identify the promotion of pericyte-endothelial cell interactions needed for brain tumor vascularization by NG2 proteoglycan-dependent recruitment of tumor macrophages
|Yotsumoto F,You WK,Cejudo-Martin P,Kucharova K,Sakimura K,Stallcup WB||Oncoimmunology (4:null)||2015|
Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
MA3105 was used in immunohistochemistry to investigate the role of neutrophil during vascular remodeling after Mycoplasma infection of mouse airways
|Baluk P,Phillips K,Yao LC,Adams A,Nitschké M,McDonald DM||The American journal of pathology (184:1877)||2014|
IL-33 expands suppressive CD11b+ Gr-1(int) and regulatory T cells, including ST2L+ Foxp3+ cells, and mediates regulatory T cell-dependent promotion of cardiac allograft survival.
MA3105 was used in immunohistochemistry to study the effect of IL-33 on suppressive CD11b+ Gr-1 int and regulatory T cells and its role in cardiac allograft survival
|Turnquist HR,Zhao Z,Rosborough BR,Liu Q,Castellaneta A,Isse K,Wang Z,Lang M,Stolz DB,Zheng XX,Demetris AJ,Liew FY,Wood KJ,Thomson AW||Journal of immunology (Baltimore, Md. : 1950) (187:4598)||2011|
Increased vascular delivery and efficacy of chemotherapy after inhibition of platelet-derived growth factor-B.
MA3105 was used in immunohistochemistry to study the ability of PDBF-B inhibition to increase the venous delivery and chemotherapeutic efficacy of cyclophosphoramide
|Falcon BL,Pietras K,Chou J,Chen D,Sennino B,Hanahan D,McDonald DM||The American journal of pathology (178:2920)||2011|
Context-dependent role of angiopoietin-1 inhibition in the suppression of angiogenesis and tumor growth: implications for AMG 386, an angiopoietin-1/2-neutralizing peptibody.
MA3105 was used in immunohistochemistry to study the effect of inhibition of angiotensin-1 on angiogenesis and tumor growth and the significance for a novel peptide-Fc fusion dual angiotensin1/2 inhibitor
|Coxon A,Bready J,Min H,Kaufman S,Leal J,Yu D,Lee TA,Sun JR,Estrada J,Bolon B,McCabe J,Wang L,Rex K,Caenepeel S,Hughes P,Cordover D,Kim H,Han SJ,Michaels ML,Hsu E,Shimamoto G,Cattley R,Hurh E,Nguyen L,Wang SX,Ndifor A,Hayward IJ,Falcón BL,McDonald DM,Li L,Boone T,Kendall R,Radinsky R,Oliner JD||Molecular cancer therapeutics (9:2641)||2010|
Rapid remodeling of airway vascular architecture at birth.
MA3105 was used in immunohistochemistry to investigate the plasticity of airway vasculature
|Ni A,Lashnits E,Yao LC,Baluk P,McDonald DM||Developmental dynamics : an official publication of the American Association of Anatomists (239:2354)||2010|
Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth.
MA3105 was used in immunohistochemistry to study the complimentary effects of concurrent inhibition of angiopoietin-2 and VEGF on tumour growth in a human colon carcinoma mouse xenofraft model
|Hashizume H,Falcón BL,Kuroda T,Baluk P,Coxon A,Yu D,Bready JV,Oliner JD,McDonald DM||Cancer research (70:2213)||2010|
Steroid-resistant lymphatic remodeling in chronically inflamed mouse airways.
MA3105 was used in immunohistochemistry to investigate the reversibility of lymphatic remodeling in chronically inflamed airways
|Yao LC,Baluk P,Feng J,McDonald DM||The American journal of pathology (176:1525)||2010|
Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1.
MA3105 was used in immunohistochemistry to study the role of RA-GEF-1 in the vascular development
|Kanemura H,Satoh T,Bilasy SE,Ueda S,Hirashima M,Kataoka T||Biochemical and biophysical research communications (387:754)||2009|
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
MA3105 was used in immunohistochemistry to determine the role of TNF-alpha in vessel remodeling in murine sustained airway inflammation
|Baluk P,Yao LC,Feng J,Romano T,Jung SS,Schreiter JL,Yan L,Shealy DJ,McDonald DM||The Journal of clinical investigation (119:2954)||2009|
alpha5beta1 Integrin blockade inhibits lymphangiogenesis in airway inflammation.
MA3105 was used in immunohistochemistry to investigate the effect of alpha 5 beta 1 integrin blockade on lymphangiogenesis during inflammation
|Okazaki T,Ni A,Ayeni OA,Baluk P,Yao LC,Vossmeyer D,Zischinsky G,Zahn G,Knolle J,Christner C,McDonald DM||The American journal of pathology (174:2378)||2009|
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
MA3105 was used in immunohistochemistry to study the effect of fibroblasts on leukocyte survival
|Filer A,Parsonage G,Smith E,Osborne C,Thomas AM,Curnow SJ,Rainger GE,Raza K,Nash GB,Lord J,Salmon M,Buckley CD||Arthritis and rheumatism (54:2096)||2006|
Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors.
MA3105 was used in immunohistochemistry (frozen) to characterize the anti-tumor effects of OXA-11.
|Moen I,Gebre M,Alonso-Camino V,Chen D,Epstein D,McDonald DM||Clinical and experimental metastasis (32:799)||2015|
Involvement of endothelial apoptosis underlying chronic obstructive pulmonary disease-like phenotype in adiponectin-null mice: implications for therapy.
MA3105 was used in immunocytochemistry to study the COPD-like phenotype in adiponectin-null mice.
|Nakanishi K,Takeda Y,Tetsumoto S,Iwasaki T,Tsujino K,Kuhara H,Jin Y,Nagatomo I,Kida H,Goya S,Kijima T,Maeda N,Funahashi T,Shimomura I,Tachibana I,Kawase I||American journal of respiratory and critical care medicine (183:1164)||2011|
Lymphatic vessel assembly is impaired in Aspp1-deficient mouse embryos.
MA3105 was used in ELISA to investigate the role of lymphatic vessel assembly
|Hirashima M,Sano K,Morisada T,Murakami K,Rossant J,Suda T||Developmental biology (316:149)||2008|
Role of PECAM-1 (CD31) in neutrophil transmigration in murine models of liver and peritoneal inflammation.
MA3105 was used in blocking/activating experiment to assess the function of platelet endothelial cell adhesion molecule-1 in neutrophil migration
|Chosay JG,Fisher MA,Farhood A,Ready KA,Dunn CJ,Jaeschke H||The American journal of physiology (274:G776)||1998|