|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Murine macrophage precursor hybrid W1C3cell hybrid|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-40074 detects CD31 from mouse samples.
MA1-40074 has been successfully used in ELISA, immunohistochemistry (paraffin and frozen), immunoprecipitation, and flow cytometry applications. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting, working dilution is 1:50.
The MA1-40074 immunogen is mouse PECAM-1/CD31.
The monoclonal antibody ER-MP12 reacts with an antigen present on cell subpopulations of adult mouse bone marrow, including pluripotent hematopoietic stem cells. The mouse hematopoietic progenitor cell antigen is Platelet-Endothelial Cell Adhesion Molecule (PECAM)-1 (CD31) of 113 kDa. PECAM-1 is a member of the immunoglobulin superfamily.This heavily glycosylated protein is found in the entire vascular endothelium of adult mice and functions in mediating cellular adhesion by heterophilic and homophilic mechanisms. ER-MP12 reacts with the major population of colony forming unit macrophage (CFU-M) precursor cells, with subpopulation of pre CFU-M and monoblasts, and with precursor cells of granulocytes. PECAM-1 is deteted within the lyphopoietic islands in the spleen of newborn (day 12) and the bone marrow of adult mice. Capillary endothelial cells of adult mice also express PECAM-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.
MA1-40074 was used in immunohistochemistry to examine the role of lymph node stromal cells in immune responses
|Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE||eLife (3:null)||2014|