Immunofluorescent analysis of CD31 (green) showing staining in the membrane and cytoplasm of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD31 monoclonal antibody (Product # MA3100) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human|
|Published species reactivity||Rat, Bovine, Human|
|Host / Isotype||Mouse / IgG2a|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
|Blocking Assay (BLOCK)||See 5 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
MA3100 targets CD31 in ICC/IF and ACS applications and shows reactivity with Human and Bovine samples.
The MA3100 immunogen is human PECAM-1.
MA3100 detects CD31 which has a predicted molecular weight of approximately 80 kDa.
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aberrant proliferation in CXCR7+ endothelial cells via degradation of the retinoblastoma protein.
MA3100 was used in immunocytochemistry to study the role of proteasomal degradation of retinoblastoma protein in the proliferation of CXCR7+ endothelial cells
|Totonchy JE,Osborn JM,Botto S,Clepper L,Moses AV||PloS one (8:null)||2013|
Transmigration across activated endothelium induces transcriptional changes, inhibits apoptosis, and decreases antimicrobial protein expression in human monocytes.
MA3100 was used in blocking or activating experiment to investigate the effect of transmigration on monocyte function modulation
|Williams MR,Sakurai Y,Zughaier SM,Eskin SG,McIntire LV||Journal of leukocyte biology (86:1331)||2009|
Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.
MA3100 was used in blocking/activating experiment to investigate the influence of calcineurin inhibitors on dendritic cell adhesion and transmigration to allogeneic endothelial cells
|Schlichting CL,Schareck WD,Weis M||Journal of cardiovascular pharmacology (46:250)||2005|
Cell contact-dependent activation of alpha3beta1 integrin modulates endothelial cell responses to thrombospondin-1.
MA3100 was used in blocking/activating experiment to study the effect of alpha3beta1 integrin on endothelial cell responses to thrombospondin-1
|Chandrasekaran L,He CZ,Al-Barazi H,Krutzsch HC,Iruela-Arispe ML,Roberts DD||Molecular biology of the cell (11:2885)||2000|
A standardized, a computer-assisted in vitro assay for the assessment of neutrophil transmigration across endothelial monolayers.
MA3100 was used in blocking/activating experiment to investigate the efficiency of a novel assay for the detection of transmigrated neutrophils across endothelial monolayers
|Gröger M,Matsumura T,Kohrgruber N,Maurer D,Wolff K,Petzelbauer P||Journal of immunological methods (222:101)||1999|
L- and P-selectins, but not CD49d (VLA-4) integrins, mediate monocyte initial attachment to TNF-alpha-activated vascular endothelium under flow in vitro.
MA3100 was used in blocking/activating experiment to investigate the mechanism for the monocyte initial attachment to vascular endothelium
|Luscinskas FW,Ding H,Tan P,Cumming D,Tedder TF,Gerritsen ME||Journal of immunology (Baltimore, Md. : 1950) (157:326)||1996|
Adipocytes as immune cells: differential expression of TWEAK, BAFF, and APRIL and their receptors (Fn14, BAFF-R, TACI, and BCMA) at different stages of normal and pathological adipose tissue development.
MA3100 was used in flow cytometry to investigate the expression level of multiple genes in adipose tissues
|Alexaki VI,Notas G,Pelekanou V,Kampa M,Valkanou M,Theodoropoulos P,Stathopoulos EN,Tsapis A,Castanas E||Journal of immunology (Baltimore, Md. : 1950) (183:5948)||2009|
Endothelial cell activation by myeloblasts: molecular mechanisms of leukostasis and leukemic cell dissemination.
MA3100 was used in flow cytometry to study the effect of myeloblasts on endothelial cell activation
|Stucki A,Rivier AS,Gikic M,Monai N,Schapira M,Spertini O||Blood (97:2121)||2001|
Integrin alpha4beta1-VCAM-1-mediated adhesion between endothelial and mural cells is required for blood vessel maturation.
MA3100 was used in immunohistochemistry to study the role of of integrin alpha4beta1-VCAM-1-mediated adhesion in blood vessel maturation
|Garmy-Susini B,Jin H,Zhu Y,Sung RJ,Hwang R,Varner J||The Journal of clinical investigation (115:1542)||2005|
A chemokine-dependent stromal induction mechanism for aberrant lymphocyte accumulation and compromised lymphatic return in rheumatoid arthritis.
MA3100 was used in immunohistochemistry to study the mechanism for aberrant lymphocyte accumulation and compromised lymphatic return in chronic inflammation
|Burman A,Haworth O,Hardie DL,Amft EN,Siewert C,Jackson DG,Salmon M,Buckley CD||Journal of immunology (Baltimore, Md. : 1950) (174:1693)||2005|
Functional roles for PECAM-1 (CD31) and VE-cadherin (CD144) in tube assembly and lumen formation in three-dimensional collagen gels.
MA3100 was used in western blot to investigate the role of CD31 and CD144 in tube assembly and lumen formation in three-dimensional collagen gels
|Yang S,Graham J,Kahn JW,Schwartz EA,Gerritsen ME||The American journal of pathology (155:887)||1999|
Endotoxin-inducible granulocyte-mediated hepatocytotoxicity requires adhesion and serine protease release.
MA3100 was used in ELISA to study the role of cell adhesion and serine protease in hepatocytotoxicity
|Sauer A,Hartung T,Aigner J,Wendel A||Journal of leukocyte biology (60:633)||1996|
PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily.
MA3100 was used in immunoprecipitation to identify and characterize PECAM-1 gene
|Newman PJ,Berndt MC,Gorski J,White GC,Lyman S,Paddock C,Muller WA||Science (New York, N.Y.) (247:1219)||1990|