|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of mouse CD31 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
CD31 is a glycoprotein expressed on endothelial cells and in platelets. It is known to be involved in cell signaling and cell adhesion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Marine natural products-inspired phenylmethylene hydantoins with potent in vitro and in vivo antitumor activities via suppression of Brk and FAK signaling.
PA5-32321 was used in immunohistochemistry to describe the synthesis of second-generation Z-4-hydroxyphenylmethylene hydantoin derivatives with enhanced antitumor activities
|Sallam AA,Mohyeldin MM,Foudah AI,Akl MR,Nazzal S,Meyer SA,Liu YY,El Sayed KA||Organic and biomolecular chemistry (12:5295)||2014|
Olive phenolics as c-Met inhibitors: (-)-Oleocanthal attenuates cell proliferation, invasiveness, and tumor growth in breast cancer models.
PA5-32321 was used in immunohistochemistry to study the role of c-Met in the therapeutic effects of (-)-oleocanthal in models of breast cancer
|Akl MR,Ayoub NM,Mohyeldin MM,Busnena BA,Foudah AI,Liu YY,Sayed KA||PloS one (9:null)||2014|
The marine-derived sipholenol A-4-O-3',4'-dichlorobenzoate inhibits breast cancer growth and motility in vitro and in vivo through the suppression of Brk and FAK signaling.
PA5-32321 was used in immunohistochemistry to study the roles of Brk and FAK in the mechanism by which a marine sipholenol inhibits the growth and motility of breast cancer cells
|Akl MR,Foudah AI,Ebrahim HY,Meyer SA,El Sayed KA||Marine drugs (12:2282)||2014|