Immunofluorescent analysis of CD31/PECAM-1 using a CD31/PECAM-1 monoclonal antibody (Product # MA1-26196). DAPI-stained rat vascular endothelial cells were immunoreactive for CD31/PECAM (red) and the anandamide hydrolyzing enzyme, fatty acid amide hydrolase (FAAH) (green).
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100 (10 µl for 10^6 cells in 100 µl)|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26196 detects CD31 from human, Cynomolgus monkey, and Rhesus monkey samples.
MA1-26196 has been successfully used in flow cytometry, immunocytochemistry and immunoprecipitation procedures. In FACS use 10 µl of the suggested working dilution to label 10^6 cels in 100 µl.
CD31 antibodies are a superior marker for angiogenesis, when compared to Factor VIII and CD34 antibodies. CD31 has been used to measure angiogenesis in association with tumor recurrence. CD31 used in a panel with CD34 and Factor VIII has also been used to mark Kaposi's sarcoma and angiosarcomas. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers.
MA1-26196 was used in flow cytometry to determine the phenotype and function of stem/progenitor cells in normal mammary epithelial cell populations and their malignant counterparts
|Ghebeh H,Sleiman GM,Manogaran PS,Al-Mazrou A,Barhoush E,Al-Mohanna FH,Tulbah A,Al-Faqeeh K,Adra CN||BMC cancer (13:null)||2013|
Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes.
MA1-26196 was used in immunohistochemistry to study the use of luminescent dendrimer lanthanide complexes for in vivo tumor imaging
|Alcala MA,Shade CM,Uh H,Kwan SY,Bischof M,Thompson ZP,Gogick KA,Meier AR,Strein TG,Bartlett DL,Modzelewski RA,Lee YJ,Petoud S,Brown CK||Biomaterials (32:9343)||2011|
Luminescence targeting and imaging using a nanoscale generation 3 dendrimer in an in vivo colorectal metastatic rat model.
MA1-26196 was used in immunohistochemistry to study the application of generation 3 polyamidoamine 4-amino-1,8-naphthalimide dendrimer containing 8 europium ions (Eu-G3P4A18N) for luminescence imaging
|Alcala MA,Kwan SY,Shade CM,Lang M,Uh H,Wang M,Weber SG,Bartlett DL,Petoud S,Lee YJ||Nanomedicine : nanotechnology, biology, and medicine (7:249)||2011|