Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (390), PerCP-eFluor™ 710, eBioscience™

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Antibody Testing Data (1)
Published Figures (96)

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 97

CD31 (PECAM-1) Antibody (46-0311-82) in Flow

Staining of BALB/c bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.06 µg of Anti-Mouse CD31 (PECAM-1) PerCP-eFluor® 710 (right). Cells in the small scatter population were used for analysis.

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Product Details

46-0311-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 81 publications 81 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

390

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834429

Product Specific Information

Description: The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This 390 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 390 antibody has been tested by flow cytometric analysis of mouse spleen and bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Type H vessel/platelet-derived growth factor receptor β⁺ perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damage.

Cell proliferation

Li J,Chen X,Ren L,Chen X,Wu T,Wang Y,Ren X,Cheng B,Xia J

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency.

Nature communications

Athanasouli P,Balli M,De Jaime-Soguero A,Boel A,Papanikolaou S,van der Veer BK,Janiszewski A,Vanhessche T,Francis A,El Laithy Y,Nigro AL,Aulicino F,Koh KP,Pasque V,Cosma MP,Verfaillie C,Zwijsen A,Heindryckx B,Nikolaou C,Lluis F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

Isolation and in vitro characterization of murine young-adult long bone skeletal progenitors.

Frontiers in endocrinology

Loopmans S,Stockmans I,Carmeliet G,Stegen S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Synovial Fibroblast Sialylation Regulates Cell Migration and Activation of Inflammatory Pathways in Arthritogenesis.

Frontiers in immunology

Wang Y,Pan P,Khan A,Çil Ç,Pineda MA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function.

Nature communications

Wang Y,Elsherbiny A,Kessler L,Cordero J,Shi H,Serke H,Lityagina O,Trogisch FA,Mohammadi MM,El-Battrawy I,Backs J,Wieland T,Heineke J,Dobreva G

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Nov 04 00:00:00 EDT 2022

Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration.

Cell death and differentiation

Deng CL,Hu CB,Ling ST,Zhao N,Bao LH,Zhou F,Xiong YC,Chen T,Sui BD,Yu XR,Hu CH

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

IL1β Promotes Immune Suppression in the Tumor Microenvironment Independent of the Inflammasome and Gasdermin D.

Cancer immunology research

Kiss M,Vande Walle L,Saavedra PHV,Lebegge E,Van Damme H,Murgaski A,Qian J,Ehling M,Pretto S,Bolli E,Keirsse J,Bardet PMR,Arnouk SM,Elkrim Y,Schmoetten M,Brughmans J,Debraekeleer A,Fossoul A,Boon L,Raes G,van Loo G,Lambrechts D,Mazzone M,Beschin A,Wullaert A,Lamkanfi M,Van Ginderachter JA,Laoui D

46-0311-82 was used in Flow Cytometry to investigate roles for IL1β in driving tumor progression in two distinct mouse tumor models.

Mon Mar 01 00:00:00 EST 2021

Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue.

Cellular and molecular life sciences : CMLS

Caputo T,Tran VDT,Bararpour N,Winkler C,Aguileta G,Trang KB,Giordano Attianese GMP,Wilson A,Thomas A,Pagni M,Guex N,Desvergne B,Gilardi F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

SORLA is required for insulin-induced expansion of the adipocyte precursor pool in visceral fat.

The Journal of cell biology

Schmidt V,Horváth C,Dong H,Blüher M,Qvist P,Wolfrum C,Willnow TE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

Tendon and motor phenotypes in the mouse model of recessive osteogenesis imperfecta.

eLife

Grol MW,Haelterman NA,Lim J,Munivez EM,Archer M,Hudson DM,Tufa SF,Keene DR,Lei K,Park D,Kuzawa CD,Ambrose CG,Eyre DR,Lee BH

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization.

JCI insight

Kashgari G,Venkatesh S,Refuerzo S,Pham B,Bayat A,Klein RH,Ramos R,Ta AP,Plikus MV,Wang PH,Andersen B

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

The IRE1/XBP1 signaling axis promotes skeletal muscle regeneration through a cell non-autonomous mechanism.

eLife

Roy A,Tomaz da Silva M,Bhat R,Bohnert KR,Iwawaki T,Kumar A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Protocols for isolation and characterization of mouse placental hemogenic endothelial cells.

STAR protocols

Liang G,Huang B,Wang F,Liu F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Loss of α2-6 sialylation promotes the transformation of synovial fibroblasts into a pro-inflammatory phenotype in arthritis.

Nature communications

Wang Y,Khan A,Antonopoulos A,Bouché L,Buckley CD,Filer A,Raza K,Li KP,Tolusso B,Gremese E,Kurowska-Stolarska M,Alivernini S,Dell A,Haslam SM,Pineda MA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Apr 20 00:00:00 EDT 2021

Mice with induced pulmonary morbidities display severe lung inflammation and mortality following exposure to SARS-CoV-2.

JCI insight

Falach R,Bar-On L,Lazar S,Kadar T,Mazor O,Aftalion M,Gur D,Evgy Y,Shifman O,Aminov T,Israeli O,Cohen-Gihon I,Zaide G,Gutman H,Vagima Y,Makdasi E,Stein D,Rosenfeld R,Alcalay R,Zahavy E,Levy H,Glinert I,Ben-Shmuel A,Israely T,Melamed S,Politi B,Achdout H,Yitzhaki S,Kronman C,Sabo T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Jun 22 00:00:00 EDT 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Protocols for endothelial cell isolation from mouse tissues: brain, choroid, lung, and muscle.

STAR protocols

Conchinha NV,Sokol L,Teuwen LA,Veys K,Dumas SJ,Meta E,García-Caballero M,Geldhof V,Chen R,Treps L,Borri M,de Zeeuw P,Falkenberg KD,Dubois C,Parys M,de Rooij LPMH,Rohlenova K,Goveia J,Schoonjans L,Dewerchin M,Eelen G,Li X,Kalucka J,Carmeliet P

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Sep 17 00:00:00 EDT 2021

Testosterone metabolites differentially regulate obesogenesis and fat distribution.

Molecular metabolism

Sebo ZL,Rodeheffer MS

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Therapy-Induced Senescence Drives Bone Loss.

Cancer research

Yao Z,Murali B,Ren Q,Luo X,Faget DV,Cole T,Ricci B,Thotala D,Monahan J,van Deursen JM,Baker D,Faccio R,Schwarz JK,Stewart SA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium.

Development (Cambridge, England)

Gagniac L,Rusidzé M,Boudou F,Cagnet S,Adlanmerini M,Jeannot P,Gaide N,Giton F,Besson A,Weyl A,Gourdy P,Raymond-Letron I,Arnal JF,Brisken C,Lenfant F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Life-span Extension Drug Interventions Affect Adipose Tissue Inflammation in Aging.

The journals of gerontology. Series A, Biological sciences and medical sciences

Mau T,O'Brien M,Ghosh AK,Miller RA,Yung R

46-0311 was used in Flow cytometry/Cell sorting to study the effects of three drugs identified in the Interventions Testing Program on inflammation in gonadal white adipose tissue of HET3 mice.

Wed Jan 01 00:00:00 EST 2020

Defining the role of pulmonary endothelial cell heterogeneity in the response to acute lung injury.

eLife

Niethamer TK,Stabler CT,Leach JP,Zepp JA,Morley MP,Babu A,Zhou S,Morrisey EE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Feb 24 00:00:00 EST 2020

Enhancer remodeling promotes tumor-initiating activity in NRF2-activated non-small cell lung cancers.

Nature communications

Okazaki K,Anzawa H,Liu Z,Ota N,Kitamura H,Onodera Y,Alam MM,Matsumaru D,Suzuki T,Katsuoka F,Tadaka S,Motoike I,Watanabe M,Hayasaka K,Sakurada A,Okada Y,Yamamoto M,Suzuki T,Kinoshita K,Sekine H,Motohashi H

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Nov 20 00:00:00 EST 2020

Snail promotes the generation of vascular endothelium by breast cancer cells.

Cell death & disease

Chang Z,Zhang Y,Liu J,Zheng Y,Li H,Kong Y,Li P,Peng H,Shi Y,Cao B,Ran F,Chen Y,Song Y,Ye Q,Ding L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2020

Sierra: discovery of differential transcript usage from polyA-captured single-cell RNA-seq data.

Genome biology

Patrick R,Humphreys DT,Janbandhu V,Oshlack A,Ho JWK,Harvey RP,Lo KK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Jul 08 00:00:00 EDT 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Myh11+ microvascular mural cells and derived mesenchymal stem cells promote retinal fibrosis.

Scientific reports

Ray HC,Corliss BA,Bruce AC,Kesting S,Dey P,Mansour J,Seaman SA,Smolko CM,Mathews C,Dey BK,Owens GK,Peirce SM,Yates PA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Sep 25 00:00:00 EDT 2020

Distinct Waves from the Hemogenic Endothelium Give Rise to Layered Lymphoid Tissue Inducer Cell Ontogeny.

Cell reports

Simic M,Manosalva I,Spinelli L,Gentek R,Shayan RR,Siret C,Girard-Madoux M,Wang S,de Fabritus L,Verschoor J,Kerdiles YM,Bajenoff M,Stumm R,Golub R,van de Pavert SA

46-0311 was used in Flow cytometry/Cell sorting to identify a major contribution from embryonic hemogenic endothelium, but not the yolk sac, toward LTi progenitors.

Tue Aug 11 00:00:00 EDT 2020

Lymph Node Mesenchymal and Endothelial Stromal Cells Cooperate via the RANK-RANKL Cytokine Axis to Shape the Sinusoidal Macrophage Niche.

Immunity

Camara A,Cordeiro OG,Alloush F,Sponsel J,Chypre M,Onder L,Asano K,Tanaka M,Yagita H,Ludewig B,Flacher V,Mueller CG

46-0311 was used in Flow cytometry/Cell sorting to demonstrate that mesenchymal stromal cells created a niche environment with LECs for specialized macrophages to take up residence, complete their differentiation program, and fulfill their immune function.

Tue Jun 18 00:00:00 EDT 2019

Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy.

EMBO molecular medicine

Uribesalgo I,Hoffmann D,Zhang Y,Kavirayani A,Lazovic J,Berta J,Novatchkova M,Pai TP,Wimmer RA,László V,Schramek D,Karim R,Tortola L,Deswal S,Haas L,Zuber J,Szűcs M,Kuba K,Dome B,Cao Y,Haubner BJ,Penninger JM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

Vascular endothelial growth factor pathway promotes osseointegration and CD31^hiEMCN^hi endothelium expansion in a mouse tibial implant model: an animal study.

The bone & joint journal

Ji G,Xu R,Niu Y,Li N,Ivashkiv L,Bostrom MPG,Greenblatt MB,Yang X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart.

JCI insight

Khalil H,Kanisicak O,Vagnozzi RJ,Johansen AK,Maliken BD,Prasad V,Boyer JG,Brody MJ,Schips T,Kilian KK,Correll RN,Kawasaki K,Nagata K,Molkentin JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Aug 08 00:00:00 EDT 2019

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury.

eLife

Farbehi N,Patrick R,Dorison A,Xaymardan M,Janbandhu V,Wystub-Lis K,Ho JW,Nordon RE,Harvey RP

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Mar 26 00:00:00 EDT 2019

PDGFRα⁺ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland.

Nature communications

Joshi PA,Waterhouse PD,Kasaian K,Fang H,Gulyaeva O,Sul HS,Boutros PC,Khokha R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Apr 15 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Metastatic cancers promote cachexia through ZIP14 upregulation in skeletal muscle.

Nature medicine

Wang G,Biswas AK,Ma W,Kandpal M,Coker C,Grandgenett PM,Hollingsworth MA,Jain R,Tanji K,Lόpez-Pintado S,Borczuk A,Hebert D,Jenkitkasemwong S,Hojyo S,Davuluri RV,Knutson MD,Fukada T,Acharyya S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Targeting skeletal endothelium to ameliorate bone loss.

Nature medicine

Xu R,Yallowitz A,Qin A,Wu Z,Shin DY,Kim JM,Debnath S,Ji G,Bostrom MP,Yang X,Zhang C,Dong H,Kermani P,Lalani S,Li N,Liu Y,Poulos MG,Wach A,Zhang Y,Inoue K,Di Lorenzo A,Zhao B,Butler JM,Shim JH,Glimcher LH,Greenblatt MB

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response.

Cellular and molecular gastroenterology and hepatology

Westendorp BF,Büller NVJA,Karpus ON,van Dop WA,Koster J,Versteeg R,Koelink PJ,Snel CY,Meisner S,Roelofs JJTH,Uhmann A,Ver Loren van Themaat E,Heijmans J,Hahn H,Muncan V,Wildenberg ME,van den Brink GR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Mar 31 00:00:00 EDT 2022

Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation.

Nature communications

Zwick RK,Rudolph MC,Shook BA,Holtrup B,Roth E,Lei V,Van Keymeulen A,Seewaldt V,Kwei S,Wysolmerski J,Rodeheffer MS,Horsley V

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Sep 04 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Tumour ischaemia by interferon-γ resembles physiological blood vessel regression.

Nature

Kammertoens T,Friese C,Arina A,Idel C,Briesemeister D,Rothe M,Ivanov A,Szymborska A,Patone G,Kunz S,Sommermeyer D,Engels B,Leisegang M,Textor A,Fehling HJ,Fruttiger M,Lohoff M,Herrmann A,Yu H,Weichselbaum R,Uckert W,Hübner N,Gerhardt H,Beule D,Schreiber H,Blankenstein T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia.

Cancer cell

Passaro D,Di Tullio A,Abarrategi A,Rouault-Pierre K,Foster K,Ariza-McNaughton L,Montaner B,Chakravarty P,Bhaw L,Diana G,Lassailly F,Gribben J,Bonnet D

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Sep 11 00:00:00 EDT 2017

VEGF165b Modulates Endothelial VEGFR1-STAT3 Signaling Pathway and Angiogenesis in Human and Experimental Peripheral Arterial Disease.

Circulation research

Ganta VC,Choi M,Kutateladze A,Annex BH

46031182 was used in flow cytometry to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a

Fri Jan 20 00:00:00 EST 2017

Preexisting endothelial cells mediate cardiac neovascularization after injury.

The Journal of clinical investigation

He L,Huang X,Kanisicak O,Li Y,Wang Y,Li Y,Pu W,Liu Q,Zhang H,Tian X,Zhao H,Liu X,Zhang S,Nie Y,Hu S,Miao X,Wang QD,Wang F,Chen T,Xu Q,Lui KO,Molkentin JD,Zhou B

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Obesity reversibly depletes the basal cell population and enhances mammary epithelial cell estrogen receptor alpha expression and progenitor activity.

Breast cancer research : BCR

Chamberlin T,D'Amato JV,Arendt LM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Nov 29 00:00:00 EST 2017

Mesenchymal Stem Cell Therapy Protects Lungs from Radiation-Induced Endothelial Cell Loss by Restoring Superoxide Dismutase 1 Expression.

Antioxidants & redox signaling

Klein D,Steens J,Wiesemann A,Schulz F,Kaschani F,Röck K,Yamaguchi M,Wirsdörfer F,Kaiser M,Fischer JW,Stuschke M,Jendrossek V

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2017

Cornea lymphatics drive the CD8⁺ T-cell response to herpes simplex virus-1.

Immunology and cell biology

Gurung HR,Carr MM,Carr DJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

β3-Adrenergic Regulation of EPC Features Through Manipulation of the Bone Marrow MSC Niche.

Journal of cellular biochemistry

Vafaei R,Nassiri SM,Siavashi V

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Dynamic Patterns of Clonal Evolution in Tumor Vasculature Underlie Alterations in Lymphocyte-Endothelial Recognition to Foster Tumor Immune Escape.

Cancer research

Corey DM,Rinkevich Y,Weissman IL

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2016

Endothelial cell survival during angiogenesis requires the pro-survival protein MCL1.

Cell death and differentiation

Watson EC,Whitehead L,Adams RH,Dewson G,Coultas L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis.

Cell adhesion & migration

Hintermann E,Bayer M,Ehser J,Aurrand-Lions M,Pfeilschifter JM,Imhof BA,Christen U

46-0311 was used in Flow cytometry/Cell sorting to show that during liver fibrogenesis, JAM-B and JAM-C expression increases on the vascular endothelium.

Sun Jul 03 00:00:00 EDT 2016

A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis.

Nature communications

Jeric I,Maurer G,Cavallo AL,Raguz J,Desideri E,Tarkowski B,Parrini M,Fischer I,Zatloukal K,Baccarini M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2016

Endothelial to mesenchymal transition is common in atherosclerotic lesions and is associated with plaque instability.

Nature communications

Evrard SM,Lecce L,Michelis KC,Nomura-Kitabayashi A,Pandey G,Purushothaman KR,d'Escamard V,Li JR,Hadri L,Fujitani K,Moreno PR,Benard L,Rimmele P,Cohain A,Mecham B,Randolph GJ,Nabel EG,Hajjar R,Fuster V,Boehm M,Kovacic JC

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jun 24 00:00:00 EDT 2016

Genetic lineage tracing defines myofibroblast origin and function in the injured heart.

Nature communications

Kanisicak O,Khalil H,Ivey MJ,Karch J,Maliken BD,Correll RN,Brody MJ,J Lin SC,Aronow BJ,Tallquist MD,Molkentin JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Jul 22 00:00:00 EDT 2016

Integrin-Alpha IIb Identifies Murine Lymph Node Lymphatic Endothelial Cells Responsive to RANKL.

PloS one

Cordeiro OG,Chypre M,Brouard N,Rauber S,Alloush F,Romera-Hernandez M,Bénézech C,Li Z,Eckly A,Coles MC,Rot A,Yagita H,Léon C,Ludewig B,Cupedo T,Lanza F,Mueller CG

46-0311 was used in Flow cytometry/Cell sorting to demonstrate that stromal reticular cells activate lymphatic endothelial cells via RANKL and support the action of hematopoietic cell-derived lymphotoxin.

Thu Aug 11 00:00:00 EDT 2016

Self-renewing diploid Axin2(+) cells fuel homeostatic renewal of the liver.

Nature

Wang B,Zhao L,Fish M,Logan CY,Nusse R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Aug 13 00:00:00 EDT 2015

Host microbiota constantly control maturation and function of microglia in the CNS.

Nature neuroscience

Erny D,Hrabě de Angelis AL,Jaitin D,Wieghofer P,Staszewski O,David E,Keren-Shaul H,Mahlakoiv T,Jakobshagen K,Buch T,Schwierzeck V,Utermöhlen O,Chun E,Garrett WS,McCoy KD,Diefenbach A,Staeheli P,Stecher B,Amit I,Prinz M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Vascular endothelial growth factor c/vascular endothelial growth factor receptor 3 signaling regulates chemokine gradients and lymphocyte migration from tissues to lymphatics.

Transplantation

Iwami D,Brinkman CC,Bromberg JS

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Conditional immortalization of primary adipocyte precursor cells.

Adipocyte

Church C,Brown M,Rodeheffer MS

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Aug 10 00:00:00 EDT 2015

Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts.

Stem cell reports

Neri T,Muggeo S,Paulis M,Caldana ME,Crisafulli L,Strina D,Focarelli ML,Faggioli F,Recordati C,Scaramuzza S,Scanziani E,Mantero S,Buracchi C,Sobacchi C,Lombardo A,Naldini L,Vezzoni P,Villa A,Ficara F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Oct 13 00:00:00 EDT 2015

Rejuvenation of the muscle stem cell population restores strength to injured aged muscles.

Nature medicine

Cosgrove BD,Gilbert PM,Porpiglia E,Mourkioti F,Lee SP,Corbel SY,Llewellyn ME,Delp SL,Blau HM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

c-kit+ cells minimally contribute cardiomyocytes to the heart.

Nature

van Berlo JH,Kanisicak O,Maillet M,Vagnozzi RJ,Karch J,Lin SC,Middleton RC,Marbán E,Molkentin JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu May 15 00:00:00 EDT 2014

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

Nature

Ubil E,Duan J,Pillai IC,Rosa-Garrido M,Wu Y,Bargiacchi F,Lu Y,Stanbouly S,Huang J,Rojas M,Vondriska TM,Stefani E,Deb A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Oct 30 00:00:00 EDT 2014

Cell-autonomous heparanase modulates self-renewal and migration in bone marrow-derived mesenchymal stem cells.

Journal of biomedical science

Cheng CC,Lee YH,Lin SP,Huangfu WC,Liu IH

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Characterization of Cre recombinase activity for in vivo targeting of adipocyte precursor cells.

Stem cell reports

Krueger KC,Costa MJ,Du H,Feldman BJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Tue Dec 09 00:00:00 EST 2014

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

The expression of Sox17 identifies and regulates haemogenic endothelium.

Nature cell biology

Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller G

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Etv2 rescues Flk1 mutant embryoid bodies.

Genesis (New York, N.Y. : 2000)

Rasmussen TL,Martin CM,Walter CA,Shi X,Perlingeiro R,Koyano-Nakagawa N,Garry DJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Oct 11 00:00:00 EDT 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Deleterious effect of the IL-23/IL-17A axis and γδT cells on left ventricular remodeling after myocardial infarction.

Journal of the American Heart Association

Yan X,Shichita T,Katsumata Y,Matsuhashi T,Ito H,Ito K,Anzai A,Endo J,Tamura Y,Kimura K,Fujita J,Shinmura K,Shen W,Yoshimura A,Fukuda K,Sano M

46-0311 was used in Flow cytometry/Cell sorting to explore potential immune pathways involved in left ventricular remodelling as possible preventative therapeutic options.

Mon Oct 01 00:00:00 EDT 2012

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Auf1/Hnrnpd-deficient mice develop pruritic inflammatory skin disease.

The Journal of investigative dermatology

Sadri N,Schneider RJ

Sun Mar 01 00:00:00 EST 2009

Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: a noninvasive therapeutic regimen.

American journal of physiology. Heart and circulatory physiology

Shabbir A,Zisa D,Suzuki G,Lee T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2009

Intramuscular VEGF repairs the failing heart: role of host-derived growth factors and mobilization of progenitor cells.

American journal of physiology. Regulatory, integrative and comparative physiology

Zisa D,Shabbir A,Mastri M,Suzuki G,Lee T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 46-0311-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

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Gene Aliases: C85791; Cd31; Pecam; PECAM-1; Pecam1

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UniProt ID: (Mouse) Q08481

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Entrez Gene ID: (Mouse) 18613

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Function(s)

immunoglobulin receptor superfamily

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CD31 (PECAM-1) Monoclonal Antibody (390), PerCP-eFluor™ 710, eBioscience™

CD31 (PECAM-1) Antibody (46-0311-82) in Flow

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46-0311-82

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