Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright™ 600, eBioscience™

Product Image Gallery

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow) — BALB/c mouse bone marrow cells were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.06 µg of CD31 Monoclonal Antibody, Super Bright 600 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993).

CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Chemerin expression promotes hypoxia and necrosis in tumors by decreasing angiogenesis. ( A ) Immunostaining of CD31 and pimonidazole in LLC tumors expressing or not chemerin at day 5 after the graft. ( B , C , and D ) The relative area of necrosis (B), hypoxia (C, pimonidazole + ), and endothelial cells (D, CD31 + ) was measured on cryosections following staining with anti-pimonidazole and anti-CD31 antibodies. The results are from a representative experiment out of 3 performed (mean +- SEM, n >= 4 mice per group, * P < 0.05, Mann-Withney test). ( E ) Relative expression of the first 38 genes of an angiogenesis signature (described for human cancer types []) in LLC tumors expressing or not chemerin (left panel). The average expression level for all 38 genes is represented in the right panel. These data were extracted from RNAseq experiments. For each condition, two pools of 3 tumors, collected 5 days after the graft, were analyzed. The data (mean +- SEM) were normalized relative to WT-LLC tumors (100%).

CD31 (PECAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 3 Adipose-derived, lineage-marked Myh11+ mural cells give rise to mesenchymal stem cells (MSCs) during adaptation and growth in vitro . ( A ) Immunostained epididymal adipose tissue from Myh11 -eYFP mice indicated eYFP+ (green) lineage marker is expressed in microvascular smooth muscle cells (vSMCs) (arrowhead) and microvascular pericytes (PCs) (asterisk) along lectin + blood vessels. Scale bar, 50 um. ( B ) Immunostained adipose tissue revealed vSMC ""tire tread"" pattern on larger arterioles. Scale bar, 25 um. ( C ) PCs are wrapped around adipose capillary microvasculature. Scale bar, 10 um. ( D ) Flow cytometry analysis showed adipose Myh11+ mural cells collected from the SVF have relatively low endogenous expression of CD73, CD90, CD105, and CD146, however, after isolation via fluorescence activated cell-sorting (FACS), cultured, passage 3-5 Myh11+ mural cells significantly increased expression of CD73, CD90, CD105, and CD146 in vitro (three independent flow analyses per panel). ( E ) Graphical representation of flow cytometry analysis demonstrated significant increase of MSC surface antigens in Myh11+ mural cells after isolation from the SVF and cultured in vitro . ( F ) Flow cytometry analysis also revealed FAC-sorted and cultured passage 3-5 Myh11+ mural cells lacked expression for hematopoetic, endothelial, and macrophage markers CD11b, CD19, CD34, CD31, and CD45 (three independent flow analyses per panel). ( G , H ) Protein and genetic analysis of passage 2 My

CD31 (PECAM-1) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 2. Evaluation of the transgene expression site by X-gal staining and immunostaining of frozen suctioned kidney sections. (A) The right kidney was suctioned at -30 kPa after i.v. injection of pCpG-free-LacZ. Sham-operated mice were injected with the same amount of pCpG-free-LacZ but not suctioned. The kidney was collected at 24 h after transfection, and 10 mum-thick frozen sections were stained with X-gal reagent. Left: sham-operated group; right: renal suction group. Magnification: x20. All scale bars represent 50 mum. (B) The right kidney was suctioned at -30 kPa after i.v. injection of pZsGreen1-N1. The kidney was collected at 24 h after transfection, and 10 mum-thick frozen sections were stained with an anti-CD31 antibody and DAPI. The stained sections were observed by CLSM. Magnification: x40. Red: CD31-positive (+) endothelial cell; green: ZsGreen1; blue: nucleus. All scale bars represent 50 mum. Color version of this figure is available Online.

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC) — Figure 5. VEGF-A-induced blood flow recovery consequent to new angiogenesis and arteriogenesis is dependent on macrophage IL-1beta expression in response to acute hindlimb ischemia (A and B) Laser Doppler images of flow in the ischemic (I) and contralateral control (C) hindlimbs of control or myeloid IL-1beta -deleted mice (mIL-1beta KO) at indicated time points before and after femoral artery ligation along with quantitative analysis (B) (**, p = 0.002; ***, p < 0.0001 between control and mIL-1beta KO for each time point by ANOVA; n = 12 mice total, six males and six females). (C and D) Standard histology stained for hematoxylin and eosin of day 3 ischemic gastrocnemius muscle tissue from control or mIL-1beta KO mice along with quantification (D) of number of infiltrating inflammatory cells, indicated by yellow arrows (n = 6 mice total, three males and three females). Bar, 100 microns. (E-H) Immunofluorescence micrographs of ischemic muscle tissue at day 3 post femoral artery ligation from control or mIL-1beta KO mice along with quantitation of DAPI + CD68 + (F), DAPI + CD31 + (G), and DAPI + SMA + (H) cells (***, p = 0.0003 by t test; n = 6 mice, three males and three females). Bar, 100 microns. Data, mean +- SD.

CD31 (PECAM-1) Antibody in Western Blot (WB) — FIGURE 2 Galectin-3 is not required for VEGF-induced VEGFR2 activity. (A) HRECs transfected with siRNA targeting Gal3 (siGal3) demonstrated over 90% mRNA knockdown of Gal3 compared to cells transfected with control siRNA (siCtrl), analyzed by reverse-transcription and qPCR assay. Student T -test was applied, * P < 0.05, n = 3. (B) Lysate from HRECs transfected with siGal3 or siCtrl was analyzed by western blot and probed with an antibody against Gal3, which resulted in over 90% Gal3 protein reduction. Student T -test was applied, * P < 0.05, n = 3. (C) siCtrl and siGal3 transfected HRECs were stimulated with 10 ng/ml of VEGF for 0, 1, 5, 10, 15, and 30 min. Cell lysates were collected and analyzed by western blot for tubulin, VEGFR2, and phospho-VEGFR2 (pVEGFR2 Y1175). Protein levels for pVEGFR2 were quantified using ImageJ and normalized to total VEGFR2 and alpha-tubulin. Ordinary one-way ANOVA was applied for comparisons within groups, * P < 0.05, ** P < 0.01, *** P < 0.001, n = 3. (D) Confluent HRECs, transfected with siCtrl or siGal3, were scratched and stimulated with 10 ng/ml of VEGF. Images were taken immediately after scratching and 15 h later. Cell migration was quantified using ImageJ and normalized to the non-treatment control group. Scale bar represents 500 mum. Student T -test was applied, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 10. (E) HRECs transfected with siCtrl and siGal3 were stimulated with 10 ng/ml VEGF. Cell surface proteins were labeled with NH

Product Details

63-0311-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 6 publications 6 publications
Flow Cytometry (Flow)	0.125 µg/test	View 26 publications 26 publications

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	390
Conjugate	Super Bright™ 600 APC Biotin Brilliant UV 661 Brilliant UV 737 Brilliant Violet 421 Brilliant Violet 711 eFluor 450 FITC Functional Grade NovaFluor Blue 660-120S NovaFluor Red 685 NovaFluor Red 710 NovaFluor Yellow 610 PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 645 Unconjugated
Excitation/Emission Max	414/601 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2717031

Product Specific Information

Description: The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This 390 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 390 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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Synovial Fibroblast Sialylation Regulates Cell Migration and Activation of Inflammatory Pathways in Arthritogenesis.

Frontiers in immunology

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Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A.

Cell reports

Mantsounga CS,Lee C,Neverson J,Sharma S,Healy A,Berus JM,Parry C,Ceneri NM,López-Giráldez F,Chun HJ,Lu Q,Sellke F,Choudhary G,Morrison AR

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The IRE1/XBP1 signaling axis promotes skeletal muscle regeneration through a cell non-autonomous mechanism.

eLife

Roy A,Tomaz da Silva M,Bhat R,Bohnert KR,Iwawaki T,Kumar A

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Tue Nov 23 00:00:00 EST 2021

The antitumoral effects of chemerin are independent from leukocyte recruitment and mediated by inhibition of neoangiogenesis.

Oncotarget

Dubois-Vedrenne I,Al Delbany D,De Henau O,Robert V,Vernimmen M,Langa F,Lefort A,Libert F,Wittamer V,Parmentier M

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Tue Sep 14 00:00:00 EDT 2021

GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization.

JCI insight

Kashgari G,Venkatesh S,Refuerzo S,Pham B,Bayat A,Klein RH,Ramos R,Ta AP,Plikus MV,Wang PH,Andersen B

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Computed tomography imaging of macrophage phagocytic activity in abdominal aortic aneurysm.

Theranostics

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Fri Jul 30 00:00:00 EDT 2021

Immunity

Barkaway A,Rolas L,Joulia R,Bodkin J,Lenn T,Owen-Woods C,Reglero-Real N,Stein M,Vázquez-Martínez L,Girbl T,Poston RN,Golding M,Saleeb RS,Thiriot A,von Andrian UH,Duchene J,Voisin MB,Bishop CL,Voehringer D,Roers A,Rot A,Lämmermann T,Nourshargh S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunohistochemistry

Tue Jul 13 00:00:00 EDT 2021

Immunity

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Tue Jul 13 00:00:00 EDT 2021

Tendon and motor phenotypes in the mouse model of recessive osteogenesis imperfecta.

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Grol MW,Haelterman NA,Lim J,Munivez EM,Archer M,Hudson DM,Tufa SF,Keene DR,Lei K,Park D,Kuzawa CD,Ambrose CG,Eyre DR,Lee BH

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Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

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Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

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Tue May 11 00:00:00 EDT 2021

Loss of α2-6 sialylation promotes the transformation of synovial fibroblasts into a pro-inflammatory phenotype in arthritis.

Nature communications

Wang Y,Khan A,Antonopoulos A,Bouché L,Buckley CD,Filer A,Raza K,Li KP,Tolusso B,Gremese E,Kurowska-Stolarska M,Alivernini S,Dell A,Haslam SM,Pineda MA

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Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

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Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration.

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Blood angiopoietin-2 predicts liver angiogenesis and fibrosis in hepatitis C patients.

BMC gastroenterology

Osawa Y,Yoshio S,Aoki Y,Korenaga M,Imamura M,Oide T,Okawara M,Kawai H,Tsutsui Y,Yoshida Y,Yoshikawa S,Mori T,Yamazoe T,Kanto T

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Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

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Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue.

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Enhancer remodeling promotes tumor-initiating activity in NRF2-activated non-small cell lung cancers.

Nature communications

Okazaki K,Anzawa H,Liu Z,Ota N,Kitamura H,Onodera Y,Alam MM,Matsumaru D,Suzuki T,Katsuoka F,Tadaka S,Motoike I,Watanabe M,Hayasaka K,Sakurada A,Okada Y,Yamamoto M,Suzuki T,Kinoshita K,Sekine H,Motohashi H

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Scientific reports

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Fri Sep 25 00:00:00 EDT 2020

Myh11+ microvascular mural cells and derived mesenchymal stem cells promote retinal fibrosis.

Scientific reports

Ray HC,Corliss BA,Bruce AC,Kesting S,Dey P,Mansour J,Seaman SA,Smolko CM,Mathews C,Dey BK,Owens GK,Peirce SM,Yates PA

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Sierra: discovery of differential transcript usage from polyA-captured single-cell RNA-seq data.

Genome biology

Patrick R,Humphreys DT,Janbandhu V,Oshlack A,Ho JWK,Harvey RP,Lo KK

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Snail promotes the generation of vascular endothelium by breast cancer cells.

Cell death & disease

Chang Z,Zhang Y,Liu J,Zheng Y,Li H,Kong Y,Li P,Peng H,Shi Y,Cao B,Ran F,Chen Y,Song Y,Ye Q,Ding L

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Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium.

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Defining the role of pulmonary endothelial cell heterogeneity in the response to acute lung injury.

eLife

Niethamer TK,Stabler CT,Leach JP,Zepp JA,Morley MP,Babu A,Zhou S,Morrisey EE

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Mon Feb 24 00:00:00 EST 2020

Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart

JCI insight

Khalil H,Kanisicak O,Vagnozzi RJ,Johansen AK,Maliken BD,Prasad V,Boyer JG,Brody MJ,Schips T,Kilian KK,Correll RN,Kawasaki K,Nagata K,Molkentin JD

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Thu Aug 08 00:00:00 EDT 2019

Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy.

EMBO molecular medicine

Uribesalgo I,Hoffmann D,Zhang Y,Kavirayani A,Lazovic J,Berta J,Novatchkova M,Pai TP,Wimmer RA,László V,Schramek D,Karim R,Tortola L,Deswal S,Haas L,Zuber J,Szűcs M,Kuba K,Dome B,Cao Y,Haubner BJ,Penninger JM

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Thu Aug 01 00:00:00 EDT 2019

Apelin inhibition prevents resistance and metastasis associated with anti-angiogenic therapy.

EMBO molecular medicine

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

Vascular endothelial growth factor pathway promotes osseointegration and CD31^hiEMCN^hi endothelium expansion in a mouse tibial implant model: an animal study.

The bone & joint journal

Ji G,Xu R,Niu Y,Li N,Ivashkiv L,Bostrom MPG,Greenblatt MB,Yang X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

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Sat Jun 01 00:00:00 EDT 2019

PDGFRα⁺ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland.

Nature communications

Joshi PA,Waterhouse PD,Kasaian K,Fang H,Gulyaeva O,Sul HS,Boutros PC,Khokha R

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Mon Apr 15 00:00:00 EDT 2019

Endothelial cells in the innate response to allergens and initiation of atopic asthma.

The Journal of clinical investigation

Asosingh K,Weiss K,Queisser K,Wanner N,Yin M,Aronica M,Erzurum S

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Mon Jul 02 00:00:00 EDT 2018

β3-Adrenergic Regulation of EPC Features Through Manipulation of the Bone Marrow MSC Niche.

Journal of cellular biochemistry

Vafaei R,Nassiri SM,Siavashi V

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice.

Drug delivery

Oyama N,Fuchigami Y,Fumoto S,Sato M,Hagimori M,Shimizu K,Kawakami S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunocytochemistry

Wed Nov 01 00:00:00 EDT 2017

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice.

Drug delivery

Oyama N,Fuchigami Y,Fumoto S,Sato M,Hagimori M,Shimizu K,Kawakami S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunocytochemistry

Wed Nov 01 00:00:00 EDT 2017

Gli1⁺ Pericyte Loss Induces Capillary Rarefaction and Proximal Tubular Injury.

Journal of the American Society of Nephrology : JASN

Kramann R,Wongboonsin J,Chang-Panesso M,Machado FG,Humphreys BD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2017

ADAM17 substrate release in proximal tubule drives kidney fibrosis.

JCI insight

Kefaloyianni E,Muthu ML,Kaeppler J,Sun X,Sabbisetti V,Chalaris A,Rose-John S,Wong E,Sagi I,Waikar SS,Rennke H,Humphreys BD,Bonventre JV,Herrlich A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunohistochemistry

Thu Aug 18 00:00:00 EDT 2016

Dynamic Patterns of Clonal Evolution in Tumor Vasculature Underlie Alterations in Lymphocyte-Endothelial Recognition to Foster Tumor Immune Escape.

Cancer research

Corey DM,Rinkevich Y,Weissman IL

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2016

Heparan sulfate regulates VEGF165- and VEGF121-mediated vascular hyperpermeability.

The Journal of biological chemistry

Xu D,Fuster MM,Lawrence R,Esko JD

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunohistochemistry

Fri Jan 07 00:00:00 EST 2011

Resident vascular progenitor cells: an emerging role for non-terminally differentiated vessel-resident cells in vascular biology.

Stem cell research

Kovacic JC,Boehm M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 63-0311-82) in Immunohistochemistry

Thu Jan 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

Gene Aliases: C85791; Cd31; Pecam; PECAM-1; Pecam1

UniProt ID: (Mouse) Q08481

Entrez Gene ID: (Mouse) 18613

Molecular Function: immunoglobulin receptor superfamily

CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright™ 600, eBioscience™

Product Details

63-0311-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

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