Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD31 (PECAM-1) Monoclonal Antibody (TLD-3A12), Biotin, eBioscience™
FIGURE: 1 / 3
CD31 (PECAM-1) Antibody (13-0310-82) in Flow
Staining of rat splenocytes with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.125 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 0.125 µg of Anti-Rat CD31 (PECAM-1) Biotin (right) followed by Streptavidin APC (Product # 17-4314-82). Total viable cells were used for analysis.
Product Details
13-0310-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TLD-3A12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572763
Product Specific Information
Description: The TLD-3A12 monoclonal antibody reacts with rat CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-kDa surface protein is expressed highly by endothelial cells but at low levels on all leukocytes and platelets. Studies have demonstrated that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interactions, and signal transduction.^M
^M
Applications Reported: This TLD-3A12 antibody has been reported for use in flow cytometric analysis.^M
^M
Applications Tested: This TLD-3A12 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
^M
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule
Gene Aliases: CD31; Pecam; Pecam1
UniProt ID: (Rat) Q3SWT0
Entrez Gene ID: (Rat) 29583
positive regulation of leukocyte migration
phagocytosis
cell adhesion
Rho protein signal transduction
regulation of cell migration
negative regulation of actin filament polymerization
response to cytokine
negative regulation of GTPase activity
wound healing
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of peptidyl-tyrosine phosphorylation
diapedesis
glomerular endothelium development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support