Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), APC-eFluor™ 780, eBioscience™

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CD31 (PECAM-1) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD31 (PECAM-1) APC-eFluor® 780 (purple histogram). Cells in the monocyte gate were used for analysis.

Product Details

47-0319-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 24 publications 24 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	WM-59 (WM59)
Conjugate	APC-eFluor™ 780 APC Biotin eFluor 450 FITC Functional Grade PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10730582

Product Specific Information

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Increased infiltration and tolerised antigen-specific CD8⁺ T_EM cells in tumor but not peripheral blood have no impact on survival of HCMV⁺ glioblastoma patients.

Oncoimmunology

Bahador M,Gras Navarro A,Rahman MA,Dominguez-Valentin M,Sarowar S,Ulvestad E,Njølstad G,Lie SA,Kristoffersen EK,Bratland E,Chekenya M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

47-0319 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

Deep Mining of Complex Antibody Phage Pools Generated by Cell Panning Enables Discovery of Rare Antibodies Binding New Targets and Epitopes.

Frontiers in pharmacology

Ljungars A,Svensson C,Carlsson A,Birgersson E,Tornberg UC,Frendéus B,Ohlin M,Mattsson M

47-0319-42 was used in Immunodepletion to demonstrate that antibodies binding new targets and epitopes, not discovered through screening alone, can be discovered using described deep mining methods.

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody.

Biomolecules

Kim DG,Nguyen TTH,Kwon NH,Sung J,Lim S,Kang EJ,Lee J,Seo WY,Kim A,Chang YS,Shim H,Kim S

47-0319 was used in Flow cytometry/Cell sorting to study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms which was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen.

Wed May 27 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

Scientific reports

Mieczkowska A,Schumacher A,Filipowicz N,Wardowska A,Zieliński M,Madanecki P,Nowicka E,Langa P,Deptuła M,Zieliński J,Kondej K,Renkielska A,Buckley PG,Crossman DK,Crowley MR,Czupryn A,Mucha P,Sachadyn P,Janus Ł,Skowron P,Rodziewicz-Motowidło S,Cichorek M,Pikuła M,Piotrowski A

47-0319 was used in Flow cytometry/Cell sorting to study the impact of prolonged cell culture on the adipose-derived stem cell phenotype and their differentiation potential.

Fri Jul 27 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Prostaglandin E₂ Is Required for BMP4-Induced Mesoderm Differentiation of Human Embryonic Stem Cells.

Stem cell reports

Zhang B,He L,Liu Y,Zhang J,Zeng Q,Wang S,Fan Z,Fang F,Chen L,Lv Y,Xi J,Yue W,Li Y,Pei X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Tue Mar 13 00:00:00 EDT 2018

Impaired lymph node stromal cell function during the earliest phases of rheumatoid arthritis.

Arthritis research & therapy

Hähnlein JS,Nadafi R,de Jong T,Ramwadhdoebe TH,Semmelink JF,Maijer KI,Zijlstra IA,Maas M,Gerlag DM,Geijtenbeek TBH,Tak PP,Mebius RE,van Baarsen LGM

Mon Feb 26 00:00:00 EST 2018

Distinctive expression of T cell guiding molecules in human autoimmune lymph node stromal cells upon TLR3 triggering.

Scientific reports

Hähnlein JS,Ramwadhdoebe TH,Semmelink JF,Choi IY,Berger FH,Maas M,Gerlag DM,Tak PP,Geijtenbeek TBH,van Baarsen LGM

47-0319 was used in Flow cytometry/Cell sorting to investigate the ability of human LNSCs derived from RA, RA-risk and healthy individuals to sense and respond to pathogens.

Mon Jan 29 00:00:00 EST 2018

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Fri May 12 00:00:00 EDT 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells.

eLife

Pal D,Pertot A,Shirole NH,Yao Z,Anaparthy N,Garvin T,Cox H,Chang K,Rollins F,Kendall J,Edwards L,Singh VA,Stone GC,Schatz MC,Hicks J,Hannon GJ,Sordella R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer.

International journal of cancer

Izumi D,Ishimoto T,Miyake K,Sugihara H,Eto K,Sawayama H,Yasuda T,Kiyozumi Y,Kaida T,Kurashige J,Imamura Y,Hiyoshi Y,Iwatsuki M,Iwagami S,Baba Y,Sakamoto Y,Miyamoto Y,Yoshida N,Watanabe M,Takamori H,Araki N,Tan P,Baba H

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.

PloS one

Brandau S,Jakob M,Bruderek K,Bootz F,Giebel B,Radtke S,Mauel K,Jäger M,Flohé SB,Lang S

47-0319 was used in Flow cytometry/Cell sorting to reveal that MSCs may contribute to the resolution of infection and inflammation by promoting the anti-microbial activity of PMNs.

Tue Jun 16 00:00:00 EDT 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.

The American journal of pathology

Yuan K,Orcholski ME,Panaroni C,Shuffle EM,Huang NF,Jiang X,Tian W,Vladar EK,Wang L,Nicolls MR,Wu JY,de Jesus Perez VA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Deciphering the stromal and hematopoietic cell network of the adventitia from non-aneurysmal and aneurysmal human aorta.

PloS one

Dutertre CA,Clement M,Morvan M,Schäkel K,Castier Y,Alsac JM,Michel JB,Nicoletti A

47-0319 was used in Flow cytometry/Cell sorting to elucidate the molecular and cellular events taking place in the adventitia in the context of aneurysm.

Wed Oct 29 00:00:00 EDT 2014

Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature.

Circulation

Park TS,Bhutto I,Zimmerlin L,Huo JS,Nagaria P,Miller D,Rufaihah AJ,Talbot C,Aguilar J,Grebe R,Merges C,Reijo-Pera R,Feldman RA,Rassool F,Cooke J,Lutty G,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2014

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Thu Apr 11 00:00:00 EDT 2013

Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Park TS,Zimmerlin L,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

Cell

Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 47-0319-42) in Flow Cytometry

Fri Oct 26 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

UniProt ID: (Human) P16284

Entrez Gene ID: (Human) 5175

Molecular Function: immunoglobulin receptor superfamily