Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor™ 450, eBioscience™

CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)

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CD31 (PECAM-1) Antibody (48-0319-42) in ICC/IF

Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience... View More

CD31 (PECAM-1) Antibody in Western Blot (WB)

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)

CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD31 (PECAM-1) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

48-0319-42

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 6 publications 6 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 38 publications 38 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WM-59 (WM59)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10854276

Product Specific Information

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A novel approach for transforming breast cancer stem cells into endothelial cells.

Experimental and therapeutic medicine

Mao QQ,Ji XC,Zhang JN,Teng WF,Zhou SC

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Feb 01 00:00:00 UTC 2024

Tumor-derived mesenchymal progenitor cell-related genes in the regulation of breast cancer proliferation.

Gland surgery

Chen Y,Zhu L,Wang Y,Hu J,Zhang H,Zhu J,Gong W,Liu X,Xiao F,Li X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Wed Mar 27 00:00:00 UTC 2024

Epigenetic landscape reveals MECOM as an endothelial lineage regulator.

Nature communications

Lv J,Meng S,Gu Q,Zheng R,Gao X,Kim JD,Chen M,Xia B,Zuo Y,Zhu S,Zhao D,Li Y,Wang G,Wang X,Meng Q,Cao Q,Cooke JP,Fang L,Chen K,Zhang L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Apr 25 00:00:00 UTC 2023

MiR-451a and let-7i-5p loaded extracellular vesicles attenuate heme-induced inflammation in hiPSC-derived endothelial cells.

Frontiers in immunology

Thomas JJ,Harp KO,Bashi A,Hood JL,Botchway F,Wilson MD,Thompson WE,Stiles JK,Driss A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Wed Jan 11 00:00:00 UTC 2023

Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling.

Scientific reports

Shin HS,Thakore A,Tada Y,Pedroza AJ,Ikeda G,Chen IY,Chan D,Jaatinen KJ,Yajima S,Pfrender EM,Kawamura M,Yang PC,Wu JC,Appel EA,Fischbein MP,Woo Y,Shudo Y

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Oct 20 00:00:00 UTC 2022

Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation.

Immunity

Reglero-Real N,Pérez-Gutiérrez L,Yoshimura A,Rolas L,Garrido-Mesa J,Barkaway A,Pickworth C,Saleeb RS,Gonzalez-Nuñez M,Austin-Williams SN,Cooper D,Vázquez-Martínez L,Fu T,De Rossi G,Golding M,Voisin MB,Boulanger CM,Kubota Y,Muller WA,Tooze SA,Nightingale TD,Collinson L,Perretti M,Aksoy E,Nourshargh S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry

Tue Sep 14 00:00:00 UTC 2021

Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.

The Journal of experimental medicine

Sharma M,Turaga RC,Yuan Y,Satyanarayana G,Mishra F,Bian Z,Liu W,Sun L,Yang J,Liu ZR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry

Mon Apr 05 00:00:00 UTC 2021

Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.

The Journal of experimental medicine

Sharma M,Turaga RC,Yuan Y,Satyanarayana G,Mishra F,Bian Z,Liu W,Sun L,Yang J,Liu ZR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry

Mon Apr 05 00:00:00 UTC 2021

Modeling of endothelial cell dysfunction using human induced pluripotent stem cells derived from patients with end-stage renal disease.

Kidney research and clinical practice

Kim KW,Shin YJ,Kim BM,Cui S,Ko EJ,Lim SW,Yang CW,Chung BH

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry

Wed Dec 01 00:00:00 UTC 2021

Expression and role of interleukin-1β and associated biomarkers in deep vein thrombosis.

Experimental and therapeutic medicine

Pai RZ,Fang Q,Tian G,Zhu B,Ge X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Wed Dec 01 00:00:00 UTC 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Science advances

Li Y,Chen X,Jin R,Chen L,Dang M,Cao H,Dong Y,Cai B,Bai G,Gooding JJ,Liu S,Zou D,Zhang Z,Yang C

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Science advances

Li Y,Chen X,Jin R,Chen L,Dang M,Cao H,Dong Y,Cai B,Bai G,Gooding JJ,Liu S,Zou D,Zhang Z,Yang C

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri May 01 00:00:00 UTC 2020

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Sun Nov 01 00:00:00 UTC 2020

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Sun Nov 01 00:00:00 UTC 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Sep 28 00:00:00 UTC 2020

Pro-Fibrotic CD26-Positive Fibroblasts Are Present in Greater Abundance in Breast Capsule Tissue of Irradiated Breasts.

Aesthetic surgery journal

Borrelli MR,Irizzary D,Patel RA,Nguyen D,Momeni A,Longaker MT,Wan DC

48-0319-42 was used in Flow Cytometry to investigate the role of CD26-positive fibroblasts in the formation of breast implant capsules following radiation therapy.

Mon Mar 23 00:00:00 UTC 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Wed Jul 01 00:00:00 UTC 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri Apr 03 00:00:00 UTC 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Nov 17 00:00:00 UTC 2020

Both Human Hematoma Punctured from Pelvic Fractures and Serum Increase Muscle Resident Stem Cells Response to BMP9: A Multivariate Statistical Approach.

Journal of clinical medicine

Alinejad Y,Lauzon MA,Grenier G,Balg F,Faucheux N

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Sun Apr 19 00:00:00 UTC 2020

Functionally specialized human CD4⁺ T-cell subsets express physicochemically distinct TCRs.

eLife

Kasatskaya SA,Ladell K,Egorov ES,Miners KL,Davydov AN,Metsger M,Staroverov DB,Matveyshina EK,Shagina IA,Mamedov IZ,Izraelson M,Shelyakin PV,Britanova OV,Price DA,Chudakov DM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Dec 08 00:00:00 UTC 2020

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Cell

Zhao Q,Liu J,Deng H,Ma R,Liao JY,Liang H,Hu J,Li J,Guo Z,Cai J,Xu X,Gao Z,Su S

48-0319-42 was used in Flow cytometry/Cell sorting to identify a mitochondrial circRNA that drives metaflammation and serves as a therapeutic target for NASH.

Thu Oct 01 00:00:00 UTC 2020

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Cell

Zhao Q,Liu J,Deng H,Ma R,Liao JY,Liang H,Hu J,Li J,Guo Z,Cai J,Xu X,Gao Z,Su S

48-0319-42 was used in Flow cytometry/Cell sorting to identify a mitochondrial circRNA that drives metaflammation and serves as a therapeutic target for NASH.

Thu Oct 01 00:00:00 UTC 2020

Vascular Tumor Recapitulated in Endothelial Cells from hiPSCs Engineered to Express the SERPINE1-FOSB Translocation.

Cell reports. Medicine

van IJzendoorn DGP,Salvatori DCF,Cao X,van den Hil F,Briaire-de Bruijn IH,de Jong D,Mei H,Mummery CL,Szuhai K,Bovée JVMG,Orlova VV

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Dec 22 00:00:00 UTC 2020

Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro.

JCI insight

Mendelson A,Strat AN,Bao W,Rosston P,Fallon G,Ohrn S,Zhong H,Lobo C,An X,Yazdanbakhsh K

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry

Thu Aug 22 00:00:00 UTC 2019

Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways.

Cell death & disease

Zhang X,Hou G,Liu A,Xu H,Guan Y,Wu Y,Deng J,Cao X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry

Thu Oct 10 00:00:00 UTC 2019

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

CRISPR/Cas9‑mediated hypoxia inducible factor‑1α knockout enhances the antitumor effect of transarterial embolization in hepatocellular carcinoma.

Oncology reports

Liu Q,Fan D,Adah D,Wu Z,Liu R,Yan QT,Zhang Y,Du ZY,Wang D,Li Y,Bao SY,Liu LP

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry (Paraffin)

Thu Nov 01 00:00:00 UTC 2018

Expansion of functional personalized cells with specific transgene combinations.

Nature communications

Lipps C,Klein F,Wahlicht T,Seiffert V,Butueva M,Zauers J,Truschel T,Luckner M,Köster M,MacLeod R,Pezoldt J,Hühn J,Yuan Q,Müller PP,Kempf H,Zweigerdt R,Dittrich-Breiholz O,Pufe T,Beckmann R,Drescher W,Riancho J,Sañudo C,Korff T,Opalka B,Rebmann V,Göthert JR,Alves PM,Ott M,Schucht R,Hauser H,Wirth D,May T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Mar 08 00:00:00 UTC 2018

Prostaglandin E₂ Is Required for BMP4-Induced Mesoderm Differentiation of Human Embryonic Stem Cells.

Stem cell reports

Zhang B,He L,Liu Y,Zhang J,Zeng Q,Wang S,Fan Z,Fang F,Chen L,Lv Y,Xi J,Yue W,Li Y,Pei X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Mar 13 00:00:00 UTC 2018

Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease.

Nature communications

Moss J,Magenheim J,Neiman D,Zemmour H,Loyfer N,Korach A,Samet Y,Maoz M,Druid H,Arner P,Fu KY,Kiss E,Spalding KL,Landesberg G,Zick A,Grinshpun A,Shapiro AMJ,Grompe M,Wittenberg AD,Glaser B,Shemer R,Kaplan T,Dor Y

48-0319 was used in Flow cytometry/Cell sorting to develop a protocol for unbiased determination of the tissue origins of circulating cell-free DNA, using a reference methylation atlas of 25 human tissues and cell types.

Thu Nov 29 00:00:00 UTC 2018

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

Rotator cuff tear state modulates self-renewal and differentiation capacity of human skeletal muscle progenitor cells.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Thomas KA,Gibbons MC,Lane JG,Singh A,Ward SR,Engler AJ

48-0319 was used in Flow cytometry/Cell sorting to investigate whether muscle injury state is a crucial factor during human skeletal muscle progenitor cell expansion and differentiation ex vivo.

Tue Aug 01 00:00:00 UTC 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri May 12 00:00:00 UTC 2017

Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

Journal of translational medicine

Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Fri Mar 03 00:00:00 UTC 2017

TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells.

eLife

Pal D,Pertot A,Shirole NH,Yao Z,Anaparthy N,Garvin T,Cox H,Chang K,Rollins F,Kendall J,Edwards L,Singh VA,Stone GC,Schatz MC,Hicks J,Hannon GJ,Sordella R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Jan 16 00:00:00 UTC 2017

Increased infiltration and tolerised antigen-specific CD8⁺ T_EM cells in tumor but not peripheral blood have no impact on survival of HCMV⁺ glioblastoma patients.

Oncoimmunology

Bahador M,Gras Navarro A,Rahman MA,Dominguez-Valentin M,Sarowar S,Ulvestad E,Njølstad G,Lie SA,Kristoffersen EK,Bratland E,Chekenya M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Sep 12 00:00:00 UTC 2017

CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer.

International journal of cancer

Izumi D,Ishimoto T,Miyake K,Sugihara H,Eto K,Sawayama H,Yasuda T,Kiyozumi Y,Kaida T,Kurashige J,Imamura Y,Hiyoshi Y,Iwatsuki M,Iwagami S,Baba Y,Sakamoto Y,Miyamoto Y,Yoshida N,Watanabe M,Takamori H,Araki N,Tan P,Baba H

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2016

Oxidized macrophage migration inhibitory factor is a potential new tissue marker and drug target in cancer.

Oncotarget

Schinagl A,Thiele M,Douillard P,Völkel D,Kenner L,Kazemi Z,Freissmuth M,Scheiflinger F,Kerschbaumer RJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Tue Nov 08 00:00:00 UTC 2016

Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies.

Oncotarget

Faes S,Uldry E,Planche A,Santoro T,Pythoud C,Demartines N,Dormond O

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Tue Dec 27 00:00:00 UTC 2016

Hepatitis C virus infection induces autocrine interferon signaling by human liver endothelial cells and release of exosomes, which inhibits viral replication.

Gastroenterology

Giugliano S,Kriss M,Golden-Mason L,Dobrinskikh E,Stone AE,Soto-Gutierrez A,Mitchell A,Khetani SR,Yamane D,Stoddard M,Li H,Shaw GM,Edwards MG,Lemon SM,Gale M,Shah VH,Rosen HR

48-0319 was used in Flow cytometry/Cell sorting to demonstrate that cultured human liver sinusoidal endothelial cells produce factors that mediate immunity against hepatitis C virus during infection.

Sun Feb 01 00:00:00 UTC 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Sun Feb 01 00:00:00 UTC 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Sun Feb 01 00:00:00 UTC 2015

Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.

The American journal of pathology

Yuan K,Orcholski ME,Panaroni C,Shuffle EM,Huang NF,Jiang X,Tian W,Vladar EK,Wang L,Nicolls MR,Wu JY,de Jesus Perez VA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Jan 01 00:00:00 UTC 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

48-0319 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 UTC 2014

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

48-0319 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 UTC 2014

Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature.

Circulation

Park TS,Bhutto I,Zimmerlin L,Huo JS,Nagaria P,Miller D,Rufaihah AJ,Talbot C,Aguilar J,Grebe R,Merges C,Reijo-Pera R,Feldman RA,Rassool F,Cooke J,Lutty G,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Jan 21 00:00:00 UTC 2014

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Dec 11 00:00:00 UTC 2014

Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Park TS,Zimmerlin L,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

Cell

Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri Oct 26 00:00:00 UTC 2012

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Apr 11 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

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UniProt ID: (Human) P16284

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Entrez Gene ID: (Human) 5175

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development

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CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor™ 450, eBioscience™

CD31 (PECAM-1) Antibody (48-0319-42) in ICC/IF

Product Details

48-0319-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

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Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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