Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

34 Published Figures

30 References

View all (183) CD31 (PECAM-1) antibodies

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Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™ (Product # 48-0319-41, 48-0319-42) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Cell Junctional localization. Panel e represents no expression in MDA-MB-231. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD31 (PECAM-1) Antibody in Western Blot (WB) — Western blot was performed using Anti-CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™ (Product # 48-0319-41, 48-0319-42) and a 130 kDa band corresponding to Platelet endothelial cell adhesion molecule was observed across cell lines tested. Membrane enriched extracts (40 µg lysate) of HUVEC (Lane 1), MDA-MB-231 (Lane 2), THP-1 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD31 (PECAM-1) eFluor® 450 (purple histogram). Cells in the monocyte gate were used for analysis.

CD31 (PECAM-1) Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™ (Product # 48-0319-41, 48-0319-42) in Western Blot (Ref :https://www.nature.com/articles/onc2013214). {RE}

CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry and Immunohistochemistry

CD31 (PECAM-1) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Figure 6. Immunohistochemical analysis of microvascular density and TUNEL assay in the mouse orthotopic tumor model. Tumor tissues were acquired at 20 days after the different treatments. (A) Monoclonal anti-CD31 antibody staining of the section to examine the microvascular density with x20 magnification, and (B) CD31-positive cells. Five sections were counted for each slide. (C) TUNEL assay was performed on the SMMC-7721-Fluc-induced orthotopic hepatocellular carcinoma model in mice with x20 magnification, and (D) the percentage of TUNEL-positive cells is presented. Values represent the mean +- standard error of the mean, and are representative of four independent experiments. *P<0.05, **P<0.01. HAL, hepatic artery ligation; Ctrl, control; LV, lentivirus.

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC) — Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WM-59 (WM59)

Conjugate

eFluor® 450

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_10854276

Product Specific Information

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

UniProt ID: (Human) P16284

Entrez Gene ID: (Human) 5175

Molecular Function: immunoglobulin receptor superfamily