Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor™ 450, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

40 Published Figures

39 References

View all (192) CD31 (PECAM-1) antibodies

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Datasheet

Protocols

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CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™ (Product # 48-0319-41, 48-0319-42) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Cell Junctional localization. Panel e represents no expression in MDA-MB-231. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD31 (PECAM-1) Antibody in Western Blot (WB) — Western blot was performed using Anti-CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™ (Product # 48-0319-41, 48-0319-42) and a 130 kDa band corresponding to Platelet endothelial cell adhesion molecule was observed across cell lines tested. Membrane enriched extracts (40 µg lysate) of HUVEC (Lane 1), MDA-MB-231 (Lane 2), THP-1 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD31 (PECAM-1) eFluor® 450 (purple histogram). Cells in the monocyte gate were used for analysis.

CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 5. ProAgio eliminates angiogenic vessels, enhances blood perfusion into the tumor, and consequentially reduces hypoxia. (A-D) Representative images of IF CD31 staining (A), quantification of vessel number (B), number of branch points (C), and vessel length (D) per view field in tumor sections of 4T1 mice treated with vehicle or ProAgio. Nuclei were counterstained with DAPI (blue) . Scale bars, 100 um ( n = 6-7/group). (E and F) Representative IHC images of CD31 staining (E) and quantification of CD31 + area (F) in the breast tumor sections of MMTV-PyMT mice treated with vehicle or ProAgio. Insets, x1.5. Scale bars, 100 um ( n = 5/group). (G and H) Representative fluorescence images of lectin perfusion in tumor vessels (G) and quantification of lectin-positive area (red) per total CD31-positive area (H; green, represented by white arrows) in tumor sections of 4T1 mice treated with vehicle or ProAgio. Scale bars, 100 um ( n = 4/group). (I and J) Representative IF images of dextran leakage of tumor vessels (J) and quantification of dextran + area (I) in the breast tumor sections of 4T1 orthotopic mice. Dextran + area is presented as a percentage per total section of the CD31 + area. Scale bar, 100 um ( n = 4/group). (K) Intratumoral levels of PTX in the extracts of tumors of 4T1 mice treated with vehicle or ProAgio. PTX levels were presented as nanograms of PTX per milligrams of tumor tissue ( n = 4/group). (L and M) Representative fluorescence images of FITC-conjugated P

CD31 (PECAM-1) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Figure 6. Immunohistochemical analysis of microvascular density and TUNEL assay in the mouse orthotopic tumor model. Tumor tissues were acquired at 20 days after the different treatments. (A) Monoclonal anti-CD31 antibody staining of the section to examine the microvascular density with x20 magnification, and (B) CD31-positive cells. Five sections were counted for each slide. (C) TUNEL assay was performed on the SMMC-7721-Fluc-induced orthotopic hepatocellular carcinoma model in mice with x20 magnification, and (D) the percentage of TUNEL-positive cells is presented. Values represent the mean +- standard error of the mean, and are representative of four independent experiments. *P<0.05, **P<0.01. HAL, hepatic artery ligation; Ctrl, control; LV, lentivirus.

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC) — Gel@MSN/miR-21-5p promoted local neovascularization at the infarct site after MI. ( A ) Micro-CT angiography analysis of 3D vascular structures within the infarct zone 28 days after MI indicates that the vascular volume was significantly increased in the Gel@MSN/miR-21-5p treatment group. The vascular volume within the infarct zone was quantitatively analyzed. * P < 0.05 and *** P < 0.01. n = 3 per group. ( B ) Immunofluorescence staining for CD31 (red) identified the vascular endothelium, and staining for alpha-SMA (green) identified myofibroblasts and pericytes, showing that the cardiac capillary density in histological sections of the healing infarct zone was significantly higher in the Gel@MSN/miR-21-5p treatment group than in the other groups. The CD31 and alpha-SMA staining intensities in the above-described groups were quantitatively analyzed (scale bars, 500 mm). * P < 0.05 and *** P < 0.01. Sham, n = 3; MI/saline, n = 5; MI/agomir, n = 5; MI/Gel@MSN/miR-NC, n = 6; and MI/Gel@MSN/miR-21-5p, n = 6. The data are shown as the means +- SD. Photo credit: Yan Li, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

CD31 (PECAM-1) Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience™ (Product # 48-0319-41, 48-0319-42) in Western Blot (Ref :https://www.nature.com/articles/onc2013214). {RE}

Product Details

48-0319-42

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5 µg/mL	-
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	View 4 publications 4 publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 27 publications 27 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	WM-59 (WM59)
Conjugate	eFluor™ 450 APC APC-eFluor 780 Biotin FITC Functional Grade PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10854276

Product Specific Information

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: This WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Expression and role of interleukin-1β and associated biomarkers in deep vein thrombosis.

Experimental and therapeutic medicine

Pai RZ,Fang Q,Tian G,Zhu B,Ge X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.

The Journal of experimental medicine

Sharma M,Turaga RC,Yuan Y,Satyanarayana G,Mishra F,Bian Z,Liu W,Sun L,Yang J,Liu ZR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry

Mon Apr 05 00:00:00 EDT 2021

Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.

The Journal of experimental medicine

Sharma M,Turaga RC,Yuan Y,Satyanarayana G,Mishra F,Bian Z,Liu W,Sun L,Yang J,Liu ZR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunocytochemistry

Mon Apr 05 00:00:00 EDT 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Science advances

Li Y,Chen X,Jin R,Chen L,Dang M,Cao H,Dong Y,Cai B,Bai G,Gooding JJ,Liu S,Zou D,Zhang Z,Yang C

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Science advances

Li Y,Chen X,Jin R,Chen L,Dang M,Cao H,Dong Y,Cai B,Bai G,Gooding JJ,Liu S,Zou D,Zhang Z,Yang C

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Increased infiltration and tolerised antigen-specific CD8⁺ T_EM cells in tumor but not peripheral blood have no impact on survival of HCMV⁺ glioblastoma patients.

Oncoimmunology

Bahador M,Gras Navarro A,Rahman MA,Dominguez-Valentin M,Sarowar S,Ulvestad E,Njølstad G,Lie SA,Kristoffersen EK,Bratland E,Chekenya M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

48-0319 was used in Flow cytometry/Cell sorting to identify a subpopulation of ASCs positive for CD74 with enhanced antifibrotic effects. Compared to CD74- and unsorted (US) ASCs, CD74+ ASCs have increased expression of hepatocyte growth factor, fibroblast growth factor 2, and transforming growth factor β3 (TGF-β3) and decreased levels of TGF-β1.

Sun Nov 01 00:00:00 EDT 2020

The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis.

Stem cells translational medicine

Borrelli MR,Patel RA,Adem S,Diaz Deleon NM,Shen AH,Sokol J,Yen S,Chang EY,Nazerali R,Nguyen D,Momeni A,Wang KC,Longaker MT,Wan DC

Sun Nov 01 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Pro-Fibrotic CD26-Positive Fibroblasts Are Present in Greater Abundance in Breast Capsule Tissue of Irradiated Breasts.

Aesthetic surgery journal

Borrelli MR,Irizzary D,Patel RA,Nguyen D,Momeni A,Longaker MT,Wan DC

48-0319-42 was used in Flow Cytometry to investigate the role of CD26-positive fibroblasts in the formation of breast implant capsules following radiation therapy.

Mon Mar 23 00:00:00 EDT 2020

Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways.

Cell death & disease

Zhang X,Hou G,Liu A,Xu H,Guan Y,Wu Y,Deng J,Cao X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry

Thu Oct 10 00:00:00 EDT 2019

Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro.

JCI insight

Mendelson A,Strat AN,Bao W,Rosston P,Fallon G,Ohrn S,Zhong H,Lobo C,An X,Yazdanbakhsh K

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry

Thu Aug 22 00:00:00 EDT 2019

Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease.

Nature communications

Moss J,Magenheim J,Neiman D,Zemmour H,Loyfer N,Korach A,Samet Y,Maoz M,Druid H,Arner P,Fu KY,Kiss E,Spalding KL,Landesberg G,Zick A,Grinshpun A,Shapiro AMJ,Grompe M,Wittenberg AD,Glaser B,Shemer R,Kaplan T,Dor Y

48-0319 was used in Flow cytometry/Cell sorting to develop a protocol for unbiased determination of the tissue origins of circulating cell-free DNA, using a reference methylation atlas of 25 human tissues and cell types.

Thu Nov 29 00:00:00 EST 2018

CRISPR/Cas9‑mediated hypoxia inducible factor‑1α knockout enhances the antitumor effect of transarterial embolization in hepatocellular carcinoma.

Oncology reports

Liu Q,Fan D,Adah D,Wu Z,Liu R,Yan QT,Zhang Y,Du ZY,Wang D,Li Y,Bao SY,Liu LP

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunohistochemistry (Paraffin)

Thu Nov 01 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Prostaglandin E₂ Is Required for BMP4-Induced Mesoderm Differentiation of Human Embryonic Stem Cells.

Stem cell reports

Zhang B,He L,Liu Y,Zhang J,Zeng Q,Wang S,Fan Z,Fang F,Chen L,Lv Y,Xi J,Yue W,Li Y,Pei X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Mar 13 00:00:00 EDT 2018

Expansion of functional personalized cells with specific transgene combinations.

Nature communications

Lipps C,Klein F,Wahlicht T,Seiffert V,Butueva M,Zauers J,Truschel T,Luckner M,Köster M,MacLeod R,Pezoldt J,Hühn J,Yuan Q,Müller PP,Kempf H,Zweigerdt R,Dittrich-Breiholz O,Pufe T,Beckmann R,Drescher W,Riancho J,Sañudo C,Korff T,Opalka B,Rebmann V,Göthert JR,Alves PM,Ott M,Schucht R,Hauser H,Wirth D,May T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Mar 08 00:00:00 EST 2018

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

Rotator cuff tear state modulates self-renewal and differentiation capacity of human skeletal muscle progenitor cells.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Thomas KA,Gibbons MC,Lane JG,Singh A,Ward SR,Engler AJ

48-0319 was used in Flow cytometry/Cell sorting to investigate whether muscle injury state is a crucial factor during human skeletal muscle progenitor cell expansion and differentiation ex vivo.

Tue Aug 01 00:00:00 EDT 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri May 12 00:00:00 EDT 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

Journal of translational medicine

Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Fri Mar 03 00:00:00 EST 2017

TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells.

eLife

Pal D,Pertot A,Shirole NH,Yao Z,Anaparthy N,Garvin T,Cox H,Chang K,Rollins F,Kendall J,Edwards L,Singh VA,Stone GC,Schatz MC,Hicks J,Hannon GJ,Sordella R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies.

Oncotarget

Faes S,Uldry E,Planche A,Santoro T,Pythoud C,Demartines N,Dormond O

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Tue Dec 27 00:00:00 EST 2016

Oxidized macrophage migration inhibitory factor is a potential new tissue marker and drug target in cancer.

Oncotarget

Schinagl A,Thiele M,Douillard P,Völkel D,Kenner L,Kazemi Z,Freissmuth M,Scheiflinger F,Kerschbaumer RJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Tue Nov 08 00:00:00 EST 2016

CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer.

International journal of cancer

Izumi D,Ishimoto T,Miyake K,Sugihara H,Eto K,Sawayama H,Yasuda T,Kiyozumi Y,Kaida T,Kurashige J,Imamura Y,Hiyoshi Y,Iwatsuki M,Iwagami S,Baba Y,Sakamoto Y,Miyamoto Y,Yoshida N,Watanabe M,Takamori H,Araki N,Tan P,Baba H

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

48-0319 was used in Flow cytometry/Cell sorting to show that human esophageal myofibroblasts secrete pro-inflammatory cytokines in response to acid and TLR-4 ligands.

Mon Jun 01 00:00:00 EDT 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

48-0319 was used in Flow cytometry/Cell sorting to show that human esophageal myofibroblasts secrete pro-inflammatory cytokines in response to acid and TLR-4 ligands.

Mon Jun 01 00:00:00 EDT 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Sun Feb 01 00:00:00 EST 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Immunofluorescence

Sun Feb 01 00:00:00 EST 2015

Hepatitis C virus infection induces autocrine interferon signaling by human liver endothelial cells and release of exosomes, which inhibits viral replication.

Gastroenterology

Giugliano S,Kriss M,Golden-Mason L,Dobrinskikh E,Stone AE,Soto-Gutierrez A,Mitchell A,Khetani SR,Yamane D,Stoddard M,Li H,Shaw GM,Edwards MG,Lemon SM,Gale M,Shah VH,Rosen HR

48-0319 was used in Flow cytometry/Cell sorting to demonstrate that cultured human liver sinusoidal endothelial cells produce factors that mediate immunity against hepatitis C virus during infection.

Sun Feb 01 00:00:00 EST 2015

Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.

The American journal of pathology

Yuan K,Orcholski ME,Panaroni C,Shuffle EM,Huang NF,Jiang X,Tian W,Vladar EK,Wang L,Nicolls MR,Wu JY,de Jesus Perez VA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

48-0319 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 EDT 2014

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

48-0319 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 EDT 2014

Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature.

Circulation

Park TS,Bhutto I,Zimmerlin L,Huo JS,Nagaria P,Miller D,Rufaihah AJ,Talbot C,Aguilar J,Grebe R,Merges C,Reijo-Pera R,Feldman RA,Rassool F,Cooke J,Lutty G,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2014

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Thu Apr 11 00:00:00 EDT 2013

Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Park TS,Zimmerlin L,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

Cell

Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 48-0319-42) in Flow Cytometry

Fri Oct 26 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

UniProt ID: (Human) Q9NPB7

Entrez Gene ID: (Human) 5175

Molecular Function: immunoglobulin receptor superfamily