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Flow cytometry analysis of CD32/FCGR2 was done on human peripheral blood granulocytes. Lysed whole blood was stained with a CD32/FCGR2 mouse monoclonal antibody (Product # MA1-81209) at a concentration of 0.2mg/mL for 1 hour at room temperature. Cells were washed twice with PBS, and stained with a Goat anti-Mouse IgG1 Secondary Antibody, RPE conjugate (Product # P-21129) at a dilution of 1:10 for 20 minutes at room temperature. A separate peripheral blood sample was also stained with a mouse IgG1 isotype control, RPE conjugate (Product # MG104) also at 0.2 mg/mL. Panel A shows gating of peripheral blood cell types. Panel B shows gated, stained granulocytes analyzed for CD32 fluorescent intensity. A representative 10,000 events were acquired and analyzed for each sample.
|Tested species reactivity||Dog, Human, Non-human primate, Pig|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||K562 cell line.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||20-200 ug/ml|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:1000|
|Immunoprecipitation (IP)||20 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is lymph node.
This product contains sodium azide; removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes are suitable for this purpose.
The human CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation.CD32 is expressed by B cells, monocytes, granulocytes and platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Case study interpretation: Report from the ICCS Annual Meeting, Seattle, 2014.
MA1-81209 was used in flow cytometry to discuss how to diagnosis hematolymphoid neoplasms using flow cytometry.
|Fromm JR,Tagliente DJ,Shaver AC,Neppalli V,Craig FE||Cytometry. Part B, Clinical cytometry (88:413)||2015|
CD32; CD32 receptor 2; CDw32; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma Receptor 2; Fc gamma Receptor II; Fc gamma receptor IIa; Fc gamma receptor IIb; Fc gamma receptor IIC; Fc gamma RIIb; fc-gamma RII-a; fc-gamma RII-b; fc-gamma-RIIa; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FCGR2A; FCGR2B; FCGR2C; fcRII-a; FcRII-b; FcRII-c; FGFR2B; igG Fc receptor II-a; igG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; low affinity immunoglobulin gamma Fc region receptor II-b
CD32; CD32A; CD32B; CD32C; CDW32; FCG2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FCRIIC; IGFR2