Flow cytometry analysis of CD32/FCGR2 was done on human peripheral blood granulocytes. Lysed whole blood was stained with a CD32/FCGR2 mouse monoclonal antibody (Product # MA1-81209) at a concentration of 0.2mg/mL for 1 hour at room temperature. Cells were washed twice with PBS, and stained with a Goat anti-Mouse IgG1 Secondary Antibody, RPE conjugate (Product # P-21129) at a dilution of 1:10 for 20 minutes at room temperature. A separate peripheral blood sample was also stained with a mouse IgG1 isotype control, RPE conjugate (Product # MG104) also at 0.2 mg/mL. Panel A shows gating of peripheral blood cell types. Panel B shows gated, stained granulocytes analyzed for CD32 fluorescent intensity. A representative 10,000 events were acquired and analyzed for each sample.
|Tested species reactivity||Dog, Human, Non-human primate, Pig|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||K562 cell line.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||20-200 ug/ml|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:1000|
|Immunoprecipitation (IP)||20 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is lymph node.
This product contains sodium azide; removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes are suitable for this purpose.
The human CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation.CD32 is expressed by B cells, monocytes, granulocytes and platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Case study interpretation: Report from the ICCS Annual Meeting, Seattle, 2014.
MA1-81209 was used in flow cytometry to discuss how to diagnosis hematolymphoid neoplasms using flow cytometry.
|Fromm JR,Tagliente DJ,Shaver AC,Neppalli V,Craig FE||Cytometry. Part B, Clinical cytometry (88:413)||2015|