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Invitrogen
CD321 (F11R) Monoclonal Antibody (WK9), APC, eBioscience™
FIGURE: 1 / 1
CD321 (F11R) Antibody (17-9321-42) in Flow
Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (blue histogram) or CD321 Monoclonal Antibody, APC (purple histogram). Cells in the lymphocyte gate (left) and monocyte gate (right) were used for analysis.
Product Details
17-9321-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
WK9
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762444
Product Specific Information
Description: CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is also involved in reovirus entry into cells.
Applications Reported: This WK9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This WK9 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD321; F11 R; JAM-1; JAM-A; Junctional adhesion molecule 1; Junctional adhesion molecule A; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor
Gene Aliases: CD321; F11R; JAM; JAM1; JAMA; JCAM; KAT; PAM-1; UNQ264/PRO301
UniProt ID: (Human) Q9Y624
Entrez Gene ID: (Human) 50848
regulation of cytokine production
inflammatory response
transforming growth factor beta receptor signaling pathway
response to radiation
extracellular matrix organization
actomyosin structure organization
negative regulation of GTPase activity
positive regulation of GTPase activity
positive regulation of blood pressure
viral entry into host cell
intestinal absorption
leukocyte migration
bicellular tight junction assembly
establishment of protein localization to plasma membrane
establishment of endothelial intestinal barrier
regulation of membrane permeability
cell-cell adhesion
regulation of actin cytoskeleton reorganization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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