Invitrogen

CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Biotin, eBioscience™

Product Image Gallery

CD324 (E-Cadherin) Antibody in Flow Cytometry (Flow) — Staining of the MDCK cell line with 0.125 µg of Rat IgG1 kappa Isotype Control Biotin (Product # 13-4301-82) (blue histogram) or 0.125 µg of Anti-Human CD324 (E-Cadherin) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD324 (E-Cadherin) Antibody in Western Blot (WB) — Figure 4 ABZ treatment downregulates the snail expression in melanoma cells by increasing the accumulation of phosphorylated GSK-3 beta /Tyr216. (a) The relative transcription levels of Snail in the ABZ-treated (0.4 mu M) and control groups of A375 (left) and B16-F10 (right) melanoma cells were measured by RT-qPCR, with beta -actin as the internal control. (b) The expression of transcription factor Snail in A375 (left) and B16-F10 (right) cells was detected by western blot analysis, with beta -actin as the internal reference protein. (c-d) The expression levels of cytoplasmic proteins AKT, pAKT, GSK-3 beta , pGSK-3 beta (Ser9/Tyr216) and Snail, and nuclear protein pSnail in A375 and B16-F10 cells were also determined by western blotting, with beta -actin and PCNA as the internal controls for the cytoplasmic and nuclear proteins, respectively. The histograms show the relative density of AKT/pAKT, GSK-3 beta /pGSK-3 beta (Ser9/Tyr216), and Snail/p-Snail. (e) A375 cells were cotreated with or without MG132 and 0.4 mu M ABZ for 24 h western blot (up) was used to detect the expression levels of AKT, pGSK-3 beta /Tyr216, Snail, N-cadherin, and E-cadherin in the cytoplasm of A375 cells. The histogram (bottom) shows the relative density of AKT, pGSK-3 beta /Tyr216, Snail, E-cadherin, and N-cadherin. (f) Histogram showing the relative expression intensity of pGSK-3 beta (Ser9/Tyr216) and pAKT after immunohistochemical staining of mouse metastatic lung cancer tissues. Scale bars = 100

CD324 (E-Cadherin) Antibody in Immunocytochemistry (ICC/IF) — Figure 4 Tight junction proteins distribution in spheroids. A typical image of a spheroid. The presence of junction markers zona occludens-1 (ZO-1) and E-cadherin were confirmed via immunofluorescent staining: red, ZO-1; green, E-cadherin; blue, nuclei (DAPI).

CD324 (E-Cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 4 Fluorescence staining of epithelial markers in the lungs. Notes: Lung sections from SCID-beige mice administered intravenously with PKH26-labeled hADSCs were observed under aconfocal laser microscope. ( A ) Fluorescence of PKH-26 (red) was observed with nuclear counterstaining by DAPI (blue) (x200). ( B ) Expression of epithelial cells markers TTF-1, Pro SP-C, beta-Catenin, and CDH1 visualized by Alexa Fluor 488 (green) in PKH26-labeled hADSCs (red) (x400). Arrow shows Alexa Flour 488 and PKH-26 merged cells. Scale bar shows 50 mum. Data are representative of more than three independent experiments. Abbreviations: hADSCs, human adipose-derived mesenchymal stem cells; DAPI, 4',6-diamidino-2-phenylindole; TTF-1, thyroid transcription factor-1; SP-C, surfactant protein C; CDH1, cadherin-1.

CD324 (E-Cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 3 Late Reprogramming Markers Are Enriched in the N+E+ Population (A) Flow cytometry plot of reprogramming culture stained with Nanog-647 and E-cadherin-488. (B) RNA-Seq read counts (transcripts per million [TPM]) for E-cadherin, labeled Cdh1 in figure, (left, Y axis) and Nanog (right, Y axis) for N+E+ and N+E- populations from three independent experiments. (C) Heatmap of genes 1.5-fold differentially expressed between N+E+ and N+E- populations (set to 1) ranked by average TPM from three independent experiments. (D) Functional annotation of gene expression in N+E+ and N+E- populations. (E) Left: Immunofluorescence images of NANOG, E-cadherin, and DPPA4 on d11 of NSC reprogramming. Right: Counts from two independent experiments of Dppa4+ colonies in N+E+ and N+E- colonies are stacked. Scale bar, 50 mum. Insets, magnification of field. (F) Comparison of d14 N+ and N+E+ colonies following withdrawal of dox on the day indicated on the X axis. Counts from four independent experiments are stacked. No significant difference between N+ and N+E+ colonies by paired two-tailed t test.

CD324 (E-Cadherin) Antibody in Immunohistochemistry (IHC) — Fig. 4 Caspase-11 mediates STAT1 activation during CAC. a Western blot analysis of phosho-/total STAT1, STAT3, IkappaBalpha, and actin (loading control) in colon homogenates from AOM/DSS-treated WT and Casp11 -/- mice on day 105 post initial AOM IP injection. Each lane represents an individual mouse. b Total STAT1 and c pSTAT1 expression densitometry (relative to actin) in colon homogenates from AOM/DSS-treated WT and Casp11 -/- mice. ** p < 0.01 (two-tailed unpaired t-test). Representative immunofluorescent images of d pSTAT1; and e total STAT1; co-stained for the epithelial marker E-cadherin (red) and DAPI (blue) in distal colon sections from AOM/DSS-treated WT and Casp11 -/- mice at day 105 (scale bar = 20 mum)

CD324 (E-Cadherin) Antibody in Neutralization (Neu) — Figure 3 rLRR20 and Leptospira infection downregulated E-cadherin expression. Neutralizing anti-E-cadherin antibody (Neu-alpha-E-cad) and short interfering RNA against E-cadherin (si-E-cad) were used to verify the regulatory effects of rLRR20 on E-cadherin expression in HK2s and hRPTECs. Effect of alpha-E-cad, which inhibits the interaction between rLRR20 and E-cadherin, on the rLRR20-mediated degradation of E-cadherin in HK2s ( A ) and hRPTECs ( B ). Effect of si-E-cad on the rLRR20-mediated degradation of E-cadherin in HK2s ( C ) and hRPTECs ( D ). Effect of pathogenic ( L. santarosai ) and non-pathogenic ( L. biflexa ) Leptospira species on the E-cadherin levels in HK2s ( E ) and hRPTECs ( F ). beta-actin was used as the internal control. EMJH, Ellinghausen-McCullough-Johnson-Harris medium. *** p < 0.001; ** p < 0.01; * p < 0.05.

Product Details

13-3249-82

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-Dependent	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	-	View 11 publications 11 publications
Flow Cytometry (Flow)	0.25 µg/test	View 15 publications 15 publications
Neutralization (Neu)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	DECMA-1
Conjugate	Biotin Alexa Fluor 488 eFluor 660 Functional Grade PerCP-eFluor 710 Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1659688

Product Specific Information

Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.

Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.

E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.

The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.

Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of the MDCK cell line. Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway.

Molecular medicine reports

Li L,Hu Y,Chen D,Zhu J,Bao W,Xu X,Chen H,Chen W,Feng R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Sat Jan 01 00:00:00 EST 2022

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Western Blot

Fri Dec 31 00:00:00 EST 2021

Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis.

International journal of molecular sciences

Hsu SH,Chou LF,Hong CH,Chang MY,Tsai CY,Tian YC,Yang HY,Yang CW

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Neutralization

Sat Dec 04 00:00:00 EST 2021

EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor.

International journal of molecular sciences

Troitskaya O,Novak D,Nushtaeva A,Savinkova M,Varlamov M,Ermakov M,Richter V,Koval O

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Mon Nov 29 00:00:00 EST 2021

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

International journal of chronic obstructive pulmonary disease

Fujioka N,Kitabatake M,Ouji-Sageshima N,Ibaraki T,Kumamoto M,Fujita Y,Hontsu S,Yamauchi M,Yoshikawa M,Muro S,Ito T

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

International journal of chronic obstructive pulmonary disease

Fujioka N,Kitabatake M,Ouji-Sageshima N,Ibaraki T,Kumamoto M,Fujita Y,Hontsu S,Yamauchi M,Yoshikawa M,Muro S,Ito T

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Mon Nov 08 00:00:00 EST 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Western Blot

Thu Apr 01 00:00:00 EDT 2021

Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA).

International journal of molecular sciences

Kim JW,Mahiddine FY,Kim GA

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Western Blot

Wed Nov 25 00:00:00 EST 2020

Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis.

Cell reports

Leavitt T,Hu MS,Borrelli MR,Januszyk M,Garcia JT,Ransom RC,Mascharak S,desJardins-Park HE,Litzenburger UM,Walmsley GG,Marshall CD,Moore AL,Duoto B,Adem S,Foster DS,Salhotra A,Shen AH,Griffin M,Shen EZ,Barnes LA,Zielins ER,Maan ZN,Wei Y,Chan CKF,Wan DC,Lorenz HP,Chang HY,Gurtner GC,Longaker MT

13-3249-82 was used in Flow Cytometry to identify and characterise a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.

Tue Nov 10 00:00:00 EST 2020

Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.

Cancer cell

Gabitova-Cornell L,Surumbayeva A,Peri S,Franco-Barraza J,Restifo D,Weitz N,Ogier C,Goldman AR,Hartman TR,Francescone R,Tan Y,Nicolas E,Shah N,Handorf EA,Cai KQ,O'Reilly AM,Sloma I,Chiaverelli R,Moffitt RA,Khazak V,Fang CY,Golemis EA,Cukierman E,Astsaturov I

13-3249-82 was used in Flow Cytometry to dissect the causal relationship between endogenous cholesterol metabolism and pancreatic ductal adenocarcinoma development and differentiation.

Mon Oct 12 00:00:00 EDT 2020

Elucidating the fundamental fibrotic processes driving abdominal adhesion formation.

Nature communications

Foster DS,Marshall CD,Gulati GS,Chinta MS,Nguyen A,Salhotra A,Jones RE,Burcham A,Lerbs T,Cui L,King ME,Titan AL,Ransom RC,Manjunath A,Hu MS,Blackshear CP,Mascharak S,Moore AL,Norton JA,Kin CJ,Shelton AA,Januszyk M,Gurtner GC,Wernig G,Longaker MT

13-3249 was used in Flow cytometry/Cell sorting to present a systematic analysis of mouse and human adhesion tissues.

Thu Aug 13 00:00:00 EDT 2020

GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD.

Journal of cell science

Dixon EE,Maxim DS,Halperin Kuhns VL,Lane-Harris AC,Outeda P,Ewald AJ,Watnick TJ,Welling PA,Woodward OM

13-3249-80 was used in Immunocytochemistry to isolate the rapid changes in protein localization and gene expression that correlate with altered cell and tubule morphology during cyst initiation.

Thu Jul 16 00:00:00 EDT 2020

GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD.

Journal of cell science

Dixon EE,Maxim DS,Halperin Kuhns VL,Lane-Harris AC,Outeda P,Ewald AJ,Watnick TJ,Welling PA,Woodward OM

13-3249-80 was used in Immunocytochemistry to isolate the rapid changes in protein localization and gene expression that correlate with altered cell and tubule morphology during cyst initiation.

Thu Jul 16 00:00:00 EDT 2020

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.

Developmental cell

Vila Ellis L,Cain MP,Hutchison V,Flodby P,Crandall ED,Borok Z,Zhou B,Ostrin EJ,Wythe JD,Chen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Mon Mar 09 00:00:00 EDT 2020

Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT.

Cell reports

Bornes L,van Scheppingen RH,Beerling E,Schelfhorst T,Ellenbroek SIJ,Seinstra D,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Tue Nov 26 00:00:00 EST 2019

Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.

Nature communications

Malik V,Glaser LV,Zimmer D,Velychko S,Weng M,Holzner M,Arend M,Chen Y,Srivastava Y,Veerapandian V,Shah Z,Esteban MA,Wang H,Chen J,Schöler HR,Hutchins AP,Meijsing SH,Pott S,Jauch R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Fri Aug 02 00:00:00 EDT 2019

Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.

Cell reports

Tran KA,Pietrzak SJ,Zaidan NZ,Siahpirani AF,McCalla SG,Zhou AS,Iyer G,Roy S,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Tue May 07 00:00:00 EDT 2019

Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency.

Cell reports

Tran KA,Pietrzak SJ,Zaidan NZ,Siahpirani AF,McCalla SG,Zhou AS,Iyer G,Roy S,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Tue May 07 00:00:00 EDT 2019

Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis.

Oncogene

Flood B,Manils J,Nulty C,Flis E,Kenealy S,Barber G,Fay J,Mills KHG,Kay EW,Creagh EM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunohistochemistry

Mon Apr 01 00:00:00 EDT 2019

β-Catenin maintains lung epithelial progenitors after lung specification.

Development (Cambridge, England)

Ostrin EJ,Little DR,Gerner-Mauro KN,Sumner EA,Ríos-Corzo R,Ambrosio E,Holt SE,Forcioli-Conti N,Akiyama H,Hanash SM,Kimura S,Huang SXL,Chen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunohistochemistry

Fri Mar 09 00:00:00 EST 2018

Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.

eLife

Chua CW,Epsi NJ,Leung EY,Xuan S,Lei M,Li BI,Bergren SK,Hibshoosh H,Mitrofanova A,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Mon Jan 15 00:00:00 EST 2018

Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation.

eLife

Chua CW,Epsi NJ,Leung EY,Xuan S,Lei M,Li BI,Bergren SK,Hibshoosh H,Mitrofanova A,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Mon Jan 15 00:00:00 EST 2018

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunofluorescence

Wed Apr 05 00:00:00 EDT 2017

Identity and dynamics of mammary stem cells during branching morphogenesis.

Nature

Scheele CL,Hannezo E,Muraro MJ,Zomer A,Langedijk NS,van Oudenaarden A,Simons BD,van Rheenen J

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Thu Feb 16 00:00:00 EST 2017

Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis.

The Journal of pathology

Ören B,Urosevic J,Mertens C,Mora J,Guiu M,Gomis RR,Weigert A,Schmid T,Grein S,Brüne B,Jung M

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells.

Genes & development

Wuidart A,Ousset M,Rulands S,Simons BD,Van Keymeulen A,Blanpain C

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.

Stem cell reports

Jackson SA,Olufs ZP,Tran KA,Zaidan NZ,Sridharan R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunofluorescence

Tue Mar 08 00:00:00 EST 2016

Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer.

Nature

Zheng X,Carstens JL,Kim J,Scheible M,Kaye J,Sugimoto H,Wu CC,LeBleu VS,Kalluri R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunohistochemistry

Thu Nov 26 00:00:00 EST 2015

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis.

Nature medicine

Lovisa S,LeBleu VS,Tampe B,Sugimoto H,Vadnagara K,Carstens JL,Wu CC,Hagos Y,Burckhardt BC,Pentcheva-Hoang T,Nischal H,Allison JP,Zeisberg M,Kalluri R

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer.

Cell death & disease

Liang SQ,Marti TM,Dorn P,Froment L,Hall SR,Berezowska S,Kocher G,Schmid RA,Peng RW

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunofluorescence

Thu Jul 16 00:00:00 EDT 2015

Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons.

Scientific reports

Wang S,Wang B,Pan N,Fu L,Wang C,Song G,An J,Liu Z,Zhu W,Guan Y,Xu ZQ,Chan P,Chen Z,Zhang YA

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Immunocytochemistry

Wed Mar 18 00:00:00 EDT 2015

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

13-3249 was used in Flow cytometry/Cell sorting to provide data to show the existence of multi-potent mammary progenitor cells that have pregnancy-specific function.

Mon Dec 08 00:00:00 EST 2014

Single luminal epithelial progenitors can generate prostate organoids in culture.

Nature cell biology

Chua CW,Shibata M,Lei M,Toivanen R,Barlow LJ,Bergren SK,Badani KK,McKiernan JM,Benson MC,Hibshoosh H,Shen MM

Published figure using CD324 (E-Cadherin) monoclonal antibody (Product # 13-3249-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

13-3249 was used in Flow cytometry/Cell sorting to investigate the routes that fibroblasts take during the generation of induced pluripotent stem cells, showing that there are orderly changes in cell-surface markers.

Thu Jul 04 00:00:00 EDT 2013

DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation.

PLoS genetics

Oda M,Kumaki Y,Shigeta M,Jakt LM,Matsuoka C,Yamagiwa A,Niwa H,Okano M

13-3249 was used in Flow cytometry/Cell sorting to study DNA methylation during embryonic stem cell differentiation.

Sat Jun 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

Gene Aliases: AA960649; Arc-1; Cadherin-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO; Uvomorulin

UniProt ID: (Dog) F1PAA9, (Human) P12830, (Mouse) P09803

Entrez Gene ID: (Dog) 442858, (Human) 999, (Mouse) 12550

Molecular Function: cadherin

Function(s)

homophilic cell adhesion via plasma membrane adhesion molecules regulation of gene expression negative regulation of cell-cell adhesion negative regulation of cell migration adherens junction organization positive regulation of protein import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development extracellular matrix disassembly extracellular matrix organization neuron projection development response to drug positive regulation of transcription factor import into nucleus establishment of protein localization to plasma membrane in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis cell adhesion sensory perception of sound calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process regulation of protein localization regulation of water loss via skin regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration

Process(es)

protein binding beta-catenin binding ankyrin binding GTPase activating protein binding identical protein binding gamma-catenin binding glycoprotein binding calcium ion binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein phosphatase binding protein domain specific binding metal ion binding

CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Biotin, eBioscience™

Product Details

13-3249-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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