Invitrogen

CD326 (EpCAM) Monoclonal Antibody (1B7), eBioscience™

Product Image Gallery

CD326 (EpCAM) Antibody — Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82)

CD326 (EpCAM) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human breast tissue using 10 µg/mL Anti-Human CD326 (EpCAM) Purified followed by Anti-Mouse IgG Biotin, Avidin HRP, and DAB visualization (right).Nuclei are counterstained with hematoxyin.

CD326 (EpCAM) Antibody in Flow Cytometry (Flow) — Staining of A549 human lung carcinoma cells with 0.125 µg of Purified Mouse IgG1 K Isotype Control (Product # 14-4714-82) (open histogram) or 0.125 µg of Anti-Human CD326 (EpCAM) Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.25 µg/test

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 6 publications below

Immunofluorescence (IF)

See 2 publications below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1B7

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_795876

Product Specific Information

Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.

Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis, immunohistochemistry of formalin-fixed paraffin embedded or frozen tissue sections, and immunoprecipitation. The 1B7 antibody has also been found useful for immunoblotting, and recognizes a protein of approximately 40 kD under non-reducing conditions.

Applications Tested: This 1B7 antibody has been tested by flow cytometric analysis of A549 human lung carcinoma cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 1B7 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM is a ~40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; Epithelial Specific Antigen; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; M4S1; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; Tumor-associated calcium signal transducer 1

Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

UniProt ID: (Human) P16422

Entrez Gene ID: (Human) 4072

Molecular Function: receptor