Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology.
Applications Reported: The G8.8 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.
Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of TE-71 cells, a murine thymic epithelial cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Ep-CAM is a ~40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD326; DIAR5; EGP-2; EGP314; EGP40; Ep-CAM; EPCAM; Epithelial cell adhesion molecule; Epithelial glycoprotein 314; Epithelial Specific Antigen; KS1/4; lymphocyte antigen 74; M4S1; mEGP314; MIC18; MK-1; panepithelial glycoprotein 314; Protein 289A; TACSTD1; Trop-1 protein; TROP1; Tumor-associated calcium signal transducer 1
Gene Aliases: CD326; EGP; EGP-2; Egp314; Ep-CAM; Epcam; EpCAM1; GA733-2; gp40; Ly74; Tacsd1; Tacstd1; TROP1
UniProt ID: (Mouse) Q99JW5
Entrez Gene ID: (Mouse) 17075
Molecular Function: receptor