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Western blot analysis of CD33/gp67 using a CD33/gp67 monoclonal antibody (Product # MA5-15446) against a truncated Trx-CD33 recombinant protein (1), truncated CD33 (aa48-258)-His recombinant protein (2) and truncated CD33 (aa18-259) human IgG Fc transfected CHO-K1 cell lysate (3).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of CD33 (48-258) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15446 targets CD33 in WB applications and shows reactivity with Human samples.
The MA5-15446 immunogen is purified recombinant fragment of CD33 (48-258) expressed in E. Coli.
CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3
CD33; p67; SIGLEC-3; SIGLEC3