|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Fusion protein containing E. coli Maltose Binding Protein and residues 271-396 of human BCRP.|
|Storage buffer||tissue culture supernatant with 0.7% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (FACS)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-35031 detects ABCG2 in human samples., and does not cross-react with human MDR1, MRP1, or MRP2.
This antibody can be used on formaldehyde-fixed, paraffin-embedded human tissues following pretreatment with 10mM citric acid (pH 6.0) for 20 minutes.
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.