|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Permanent human cell line from a patient with myeloid leukaemia.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 µg/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
For Western blot, a positive control of KG-1a human leukemia cell lysate is suggested. For paraffin sections, a positive control of placenta endothelium is suggested.
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Label-free impedance detection of low levels of circulating endothelial progenitor cells for point-of-care diagnosis.
MA1-19229 was used in ELISA to evaluate a novel microfluidic method for the detection of circulating endothelial progenitor cells
|Ng SY,Reboud J,Wang KY,Tang KC,Zhang L,Wong P,Moe KT,Shim W,Chen Y||Biosensors and bioelectronics (25:1095)||2010|